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  4. DCIP hydrate sodium

DCIP hydrate sodium  (Synonyms: Dichlorophenylindophenol hydrate sodium; DCPIP hydrate sodium; Indochlorophenol hydrate sodium)

Cat. No.: HY-D0018A Purity: 98.46%
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DCIP hydrate sodium is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

For research use only. We do not sell to patients.

DCIP hydrate sodium Chemical Structure

DCIP hydrate sodium Chemical Structure

CAS No. : 283164-79-4

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100 mg USD 25 In-stock
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Based on 1 publication(s) in Google Scholar

DCIP hydrate sodium Related Antibodies

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Description

DCIP hydrate sodium is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

IC50 & Target

Aminoimidazole carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase (ATIC)[1]
Molecular Weight

308.09

Formula

C12H8Cl2NNaO3
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

O=C1C(Cl)=C/C(C=C1Cl)=N/C2=CC=C(O[Na])C=C2.O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (324.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2458 mL 16.2290 mL 32.4580 mL
5 mM 0.6492 mL 3.2458 mL 6.4916 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (8.11 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2458 mL 16.2290 mL 32.4580 mL 81.1451 mL
5 mM 0.6492 mL 3.2458 mL 6.4916 mL 16.2290 mL
10 mM 0.3246 mL 1.6229 mL 3.2458 mL 8.1145 mL
15 mM 0.2164 mL 1.0819 mL 2.1639 mL 5.4097 mL
20 mM 0.1623 mL 0.8115 mL 1.6229 mL 4.0573 mL
25 mM 0.1298 mL 0.6492 mL 1.2983 mL 3.2458 mL
30 mM 0.1082 mL 0.5410 mL 1.0819 mL 2.7048 mL
40 mM 0.0811 mL 0.4057 mL 0.8115 mL 2.0286 mL
50 mM 0.0649 mL 0.3246 mL 0.6492 mL 1.6229 mL
60 mM 0.0541 mL 0.2705 mL 0.5410 mL 1.3524 mL
80 mM 0.0406 mL 0.2029 mL 0.4057 mL 1.0143 mL
100 mM 0.0325 mL 0.1623 mL 0.3246 mL 0.8115 mL
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DCIP hydrate sodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DCIP hydrate283164-79-4Dichlorophenylindophenol hydrateDCPIP hydrateIndochlorophenol hydrateBiochemical Assay Reagentsbiochemical assayreagentInhibitorinhibitorinhibit

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