Referencia número: HY-N0439

Asiaticoside

19 Cited Publications

Revisión del cliente

Based on 19 publication(s) in Google Scholar

Asiaticoside, a trisaccaride triterpene from Centella asiatica, suppresses TGF-β/Smad signaling through inducing Smad7 and inhibiting TGF-βRI and TGF-βRII in keloid fibroblasts; Asiaticoside shows antioxidant, anti-inflammatory, and anti-ulcer properties.

Para uso exclusivo en investigación. No vendemos a pacientes.

Pureza: 99.67%
No. CAS: 16830-15-2
Fòrmula: C₄₈H₇₈O₁₉
Peso molecular:959.12

Almacenamiento:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Asiaticoside Chemical Structure

No. CAS: 16830-15-2

Tamaño

Precio

Stock

Cantidad

Amount: USD 0.00

* Seleccione Cantidad antes de agregar artículos.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- IC₅₀ & Target
- In Vitro
- In Vivo
- Ensayo clínico
Chemical Information
Publications (19)
Solvente y solubilidad
- In Vitro
- In Vivo
Protocolo
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

IC₅₀ & Target

TGF-beta/Smad^[1]

Ver todos los productos específicos de isoformas p38 MAPK :

Ver todos los productos específicos de isoformas TGF-beta/Smad :

Smad1
Smad3

Ver todos los productos específicos de isoformas Endogenous Metabolite :

In Vitro

Asiaticoside (0, 100, 250, and 500 mg/L) dose-dependently inhibits keloid fibroblasts proliferation. Asiaticoside (100, 250, and 500 mg/L) decreases the expression of collagen protein and mRNA, reduces the expression of TGF-bRI, TGF-bRII protein, and mRNA, increases the expression of Smad7, but does not affect Smad2, Smad3, Smad4, phosphorylated Smad2, and phosphorylated Smad3 in keloid fibroblasts^[1]. Asiaticoside (12.5 and 50, and 25 and 50 μg/mL) prevents endothelial cells from hypoxia-induced inhibition of cell viability and NO production. Asiaticoside (50 μg/mL) also protects endothelial cells from hypoxia-induced apoptosis and upregulates and phosphorylation of AKT/eNOS in hypoxia-exposed HPAECs^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Asiaticoside (50 mg/kg daily) blocks the development of hypoxic pulmonary hypertension (PH), cardiovascular remodeling and endothelial cell injury in rats with pulmonary hypertension^[2]. Asiaticoside (5, 15 or 45 mg/kg, p.o.) improves the learning and memory deficit, protects hippocampi against the impairment, decreases Aβ deposits in the hippocampus, and ameliorates impaired subcellular structure in rats treated with Aβ oligomers^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ensayo clínico

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

No. CAS 16830-15-2
Appearance Solid
Peso molecular 959.12
Fòrmula C₄₈H₇₈O₁₉
Color White to off-white
SMILES

C[C@@H]1CC[C@@]2(C(O[C@@H]3O[C@H](CO[C@H]4[C@H](O)[C@@H](O)[C@H](O[C@@]5([H])[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O5)[C@@H](CO)O4)[C@@H](O)[C@H](O)[C@H]3O)=O)[C@@](C6=CC[C@@]([C@](C[C@@H](O)[C@H](O)[C@]7(CO)C)(C)[C@@]7([H])CC8)([H])[C@]8(C)[C@]6(C)CC2)([H])[C@H]1C
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
- Endogenous metabolite
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (19)

All OthersCell Proliferation/Viability AssayCell Migration/Invasion AssayFlow CytometryWB

Sort by:

Journal Impact Factor

J Exp Clin Cancer Res

HOXA10 mediates epithelial-mesenchymal transition to promote gastric cancer metastasis partly via modulation of TGFB2/Smad/METTL3 signaling axis. [Abstract]

2021 Feb 9;40(1):62. PMID: 33563300
Adv Sci (Weinh)

Targeted Extracellular Vesicles Deliver Asiaticoside to Inhibit AURKB/DRP1-Mediated Mitochondrial Fission and Attenuate Hypertrophic Scar Formation. [Abstract]

2026 Apr;13(21):e17108. PMID: 41637535
Phytomedicine

Novel AMPK/ORP8-lipophagy axis: A therapeutic target for asiaticoside-mediated cardioprotection against ischemia-reperfusion injury in hyperlipidemia. [Abstract]

2025 Sep 23:148:157317. PMID: 41027151
Phytother Res

Asiaticoside hampers epithelial-mesenchymal transition by promoting PPARG expression and suppressing P2RX7-mediated TGF-β/Smad signaling in triple-negative breast cancer. [Abstract]

2023 May;37(5):1771-1786. PMID: 36444395
Pharmaceutics

Multifunctional PDO Thread Coated with Mg(OH)2/ZnO Nanoparticles and Asiaticoside for Improved Facial Lifting. [Abstract]

2023 Aug 28;15(9):2220. PMID: 37765189
Ecotoxicol Environ Saf

Asiaticoside ameliorates pyrimethanil-induced autophagy-dependent liver injury by suppressing CRHR1. [Abstract]

2025 Dec 2:308:119482. PMID: 41338089
Biomater Sci

Asiaticoside-laden silk nanofiber hydrogels to regulate inflammation and angiogenesis for scarless skin regeneration. [Abstract]

2021 Jul 27;9(15):5227-5236. PMID: 34190240

Asiaticoside purchased from MedChemExpress. Usage Cited in: Biomater Sci. 2021 Jul 27;9(15):5227-5236. [Abstract]

AFM images of Asiaticoside-laden silk nanofiber hydrogels with different Asiaticoside concentrations (0, 0.7, 1.4, 2.8 mg/ml). Scale bars 500 nm.
Int Immunopharmacol

Asiaticoside promoted ferroptosis and suppressed immune escape in gastric cancer cells by downregulating the Wnt/β-catenin pathway. [Abstract]

2024 May 10:134:112175. PMID: 38733821
Int Immunopharmacol

Asiaticoside ameliorates osteoarthritis progression through activation of Nrf2/HO-1 and inhibition of the NF-κB pathway. [Abstract]

2022 Jul:108:108864. PMID: 35623293
Mol Pharm

Machine Learning Models Identify New Inhibitors for Human OATP1B1. [Abstract]

2022 Nov 7;19(11):4320-4332. PMID: 36269563
Drug Dev Res

Asiaticoside enhances the effect of propofol on the invasion, ferroptosis and immune escape of bladder cancer. [Abstract]

2024 Sep;85(6):e22242. PMID: 39161064
Microbiol Spectr

25-Hydroxycholesterol Mediates Cholesterol Metabolism to Restrict Porcine Deltacoronavirus Infection via Suppression of Transforming Growth Factor β1. [Abstract]

2022 Dec 21;10(6):e0219822. PMID: 36314946

Asiaticoside purchased from MedChemExpress. Usage Cited in: Microbiol Spectr. 2022 Dec 21;10(6):e0219822. [Abstract]

Asiaticoside (AS) (250, 500 µg/mL; 24 h) significantly inhibits the expression of mature TGF-β1 in LLC-PK1 cells.
Naunyn Schmiedebergs Arch Pharmacol

Asiaticoside alleviates inflammation and intestinal barrier dysfunction in Crohn's disease-like colitis in a manner associated with the RhoA/ROCK/MLC pathway. [Abstract]

2026 May 16. PMID: 42142140
Toxicol Appl Pharmacol

Asiaticoside alleviates metabolic associated fatty liver disease via PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 Jun 23:514:117925. PMID: 42336298
J Biochem Mol Toxicol

Asiaticoside protected brain injury in hypertensive intracerebral hemorrhage via activation of the PI3K/AKT pathway. [Abstract]

2024 Sep;38(9):e23843. PMID: 39253885
Iran J Basic Med Sci

Asiaticoside attenuates hyperoxia-induced lung injury in vitro and in vivo. [Abstract]

2019 Jul;22(7):797-805. PMID: 32373302
Biochem Biophys Res Commun

The role and mechanism of asiaticoside in regulating smooth muscle cell mitochondrial Drp1 during neointimal hyperplasia and atherosclerosis. [Abstract]

2025 Dec 5:795:153102. PMID: 41371186
Biomed Pharmacother

Transforming growth factor-β receptor I kinase plays a crucial role in oligodendrocyte regeneration after demyelination. [Abstract]

2025 May 1:187:118094. PMID: 40315672
Heliyon

Asiaticoside inhibits breast cancer progression and tumor angiogenesis via YAP1/VEGFA signal pathway. [Abstract]

2024 Aug 29;10(18):e37169. PMID: 39309801

Asiaticoside purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Aug 29;10(18):e37169. [Abstract]

Cell growth assay in MCF-7 and MDA-MB-231 cells treated with different doses of Asiaticoside (0, 0.1, 0.5, or 2 μM; 72 h).

Asiaticoside purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Aug 29;10(18):e37169. [Abstract]

Colony formation in MCF-7 and MDA-MB-231 cells treated with different doses of Asiaticoside (0, 0.1, 0.5, or 2 μM; 72 h).

Asiaticoside purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Aug 29;10(18):e37169. [Abstract]

Transwell migration assay (24 h) in MCF-7 and MDA-MB-231 cells treated with different doses of Asiaticoside (0, 0.1, 0.5, or 2 μM).

Asiaticoside purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Aug 29;10(18):e37169. [Abstract]

Flow cytometry analysis of apoptotic events in control and Asiaticoside (2 μM, 48 h)-treated cells.

Asiaticoside purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Aug 29;10(18):e37169. [Abstract]

The culture supernatants of control- and Asiaticoside (0.1, 0.5, 2 μM, 72 h)-treated breast cancer cells were collected for incubating HUVEC cells. CCK8 assay shows a decreased cell proliferation in Asiaticoside-conditioned medium-treated HUVECs.

Asiaticoside purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Aug 29;10(18):e37169. [Abstract]

The culture supernatants of control- and Asiaticoside (0.1, 0.5, 2 μM)-treated breast cancer cells were collected for incubating HUVEC cells. Transwell assay (24-h incubation) shows the impaired cell migration in Asiaticoside-conditioned medium-treated HUVECs.

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (104.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.0426 mL

5.2131 mL

10.4262 mL

5 mM

0.2085 mL

1.0426 mL

2.0852 mL

10 mM

0.1043 mL

0.5213 mL

1.0426 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (2.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (2.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (2.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocolo

Cell Assay^[1]

Cells are seeded at a density of 2-9 × 10⁴ cells/mL into 24-well plates for cell viability, 60-mm plates for RNA and protein analysis in DMEM containing 10% FBS (DMEM/10% FBS). After 24 h, medium is removed and cells are placed in serum-free DMEM. After 48 h, different concentrations of Asiaticoside in DMEM/10% FBS are added simultaneously to cells. Control cells are grown in DMEM/10% FBS without the addition of Asiaticoside. At different time points, the fibroblasts are harvested for analysis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[3]

Rats^[3] Sprague-Dawley rats are used to establish the Alzheimer's disease (AD) model. Asiaticoside is administered orally at 5, 15 or 45 mg/kg body weight per day for 7 days. The learning and memory function of the rats are evaluated by the MWM test 7 days after surgery. The swimming pool (180 cm in diameter) is maintained at 22-24°C, and divided into four quadrants with equal size. A hidden platform is placed in the center of one quadrant. Each of the cardinal points of these four quadrants is randomLy selected as the start location. On the pre-test day, rats are allowed to swim freely for 120 sec. During the test (days 1-4), each rat is subjected to 8 trials each day. The trial begins when a rat is placed in the pool, and ended when the rat finds the platform. The escape latency is recorded. If a rat fails to find the platform within 120 sec, the trial is terminated and the escape latency is recorded as 120 sec, and the rat is guided to the platform. On day 5, the swimming path is recorded by a video recording system, and the digital images are analyzed by the water maze software. In addition, the probe test is performed, in which the platform is removed, and the rat is allowed to swim freely in the pool for 120 sec. The swimming time in the target quadrant is recorded^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (287 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Tang B, et al. Asiaticoside suppresses collagen expression and TGF-β/Smad signaling through inducing Smad7 and inhibiting TGF-βRI and TGF-βRII in keloid fibroblasts. h Dermatol Res. 2011 Oct;303(8):563-72. [Content Brief]

[2]. Wang X, et al. Effect of asiaticoside on endothelial cells in hypoxia‑induced pulmonary hypertension. Mol Med Rep. 2018 Feb;17(2):2893-2900. [Content Brief]

[3]. Zhang Z, et al. Asiaticoside ameliorates β-amyloid-induced learning and memory deficits in rats by inhibiting mitochondrial apoptosis and reducing inflammatory factors. Exp Ther Med. 2017 Feb;13(2):413-420. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.0426 mL	5.2131 mL	10.4262 mL	26.0656 mL
	5 mM	0.2085 mL	1.0426 mL	2.0852 mL	5.2131 mL
	10 mM	0.1043 mL	0.5213 mL	1.0426 mL	2.6066 mL
	15 mM	0.0695 mL	0.3475 mL	0.6951 mL	1.7377 mL
	20 mM	0.0521 mL	0.2607 mL	0.5213 mL	1.3033 mL
	25 mM	0.0417 mL	0.2085 mL	0.4170 mL	1.0426 mL
	30 mM	0.0348 mL	0.1738 mL	0.3475 mL	0.8689 mL
	40 mM	0.0261 mL	0.1303 mL	0.2607 mL	0.6516 mL
	50 mM	0.0209 mL	0.1043 mL	0.2085 mL	0.5213 mL
	60 mM	0.0174 mL	0.0869 mL	0.1738 mL	0.4344 mL
	80 mM	0.0130 mL	0.0652 mL	0.1303 mL	0.3258 mL
	100 mM	0.0104 mL	0.0521 mL	0.1043 mL	0.2607 mL

Keywords

Asiaticoside

19 Cited Publications

100 mg Obtener un presupuesto

Subscribe to the

MedChemExpress

Newsletter

Nombre del producto:
Referencia número:
Cantidad:
MCE Japan Authorized Agent:

Nombre del producto:
No. CAS:
Species:
Pureza:
Cantidad:

Asiaticoside

Apoptosis Compound Library

Immunology/Inflammation Compound Library

MAPK Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

TGF-beta/Smad Compound Library

Wnt/Hedgehog/Notch Compound Library

Natural Product Library

Clinical Compound Library

Anti-Aging Compound Library

Drug Repurposing Compound Library

Antioxidant Compound Library

Glycoside Compound Library

Oxygen Sensing Compound Library

Anti-Cardiovascular Disease Compound Library

Terpenoids Library

Pyroptosis Compound Library

Traditional Chinese Medicine Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Anti-Lung Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Neurodegenerative Disease-related Compound Library

Angiogenesis-Related Compound Library

Transcription Factor-Targeted Library

Food-Sourced Compound Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Osteogenesis Compound Library

Heterocyclic Compound Library

Pain-Related Compound Library

Cell Death Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Drug Repurposing Compound Library Plus

Clinical Compound Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Miao Ethnicity Medicine Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Mass Spectrometry Human Metabolite Library

Cosmetic Ingredient Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Natural Product Diversity Scaffold Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Asiaticoside

Ver todos los productos específicos de isoformas p38 MAPK

Ver todos los productos específicos de isoformas TGF-beta/Smad

Ver todos los productos específicos de isoformas Endogenous Metabolite

Actividad biológica

Chemical Information

Publications (19)

Solvente y solubilidad

Protocolo

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

Asiaticoside Related Classifications

HY-N0439 Related Classifications