2. Immunology/Inflammation Vitamin D Related/Nuclear Receptor NF-κB Metabolic Enzyme/Protease
  3. Glucocorticoid Receptor Reactive Oxygen Species NO Synthase
  4. Beclometasone dipropionate

Beclometasone dipropionate  (Synonyms: Beclomethasone dipropionate )

Cat. No.: HY-13571A Purity: 99.89%
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Beclometasone dipropionate, the proagent of Beclometasone, is an orally active and potent glucocorticoid recepter agonist. Beclometasone dipropionate acts via a glucocorticoid receptor and suppresses inflammation and hyperproliferation. Beclometasone dipropionate can be used for asthma .

For research use only. We do not sell to patients.

Beclometasone dipropionate Chemical Structure

Beclometasone dipropionate Chemical Structure

CAS No. : 5534-09-8

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10 mM * 1 mL in DMSO
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Based on 3 publication(s) in Google Scholar

Other Forms of Beclometasone dipropionate:

Beclometasone dipropionate Related Antibodies

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Description

Beclometasone dipropionate, the proagent of Beclometasone, is an orally active and potent glucocorticoid recepter agonist. Beclometasone dipropionate acts via a glucocorticoid receptor and suppresses inflammation and hyperproliferation. Beclometasone dipropionate can be used for asthma [1][2].

IC50 & Target

iNOS

 

In Vitro

Beclometasone dipropionate (1-100 nM; 20 min) inhibits STAT-1 expression and reduces the levels of iNOS, ROS and NT generated by rhIL-17A in 16HBE cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Beclometasone dipropionate Related Antibodies

Western Blot Analysis[2]

Cell Line: 16HBE cells
Concentration: 1, 10 and 100 nM
Incubation Time: 20 min
Result: Reduced the levels of iNOS, ROS and NT generated by rhIL-17A.
In Vivo

Beclometasone dipropionate (150 µg/kg; nebulization; male BALB/c mice) relieves asthma and decreases total cell number and relative eosinophil number[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Ten-week-old male Balb/c mice[2].
Dosage: 5 mg/kg (100 μg/ml for 60 min).
Administration: Orally at 24 h and 1 h before the LPS aerosol.
Result: Significantly (P < 0.05) inhibited the decrease of IL-10 level in BAL fluid induced by LPS exposure.
Markedly reduced the release of both MMP-2 and MMP-9.
Animal Model: Male BALB/c mice with asthma[1]
Dosage: 150 µg/kg
Administration: Nebulization
Result: Decreased total cell number and relative eosinophil number in BALF.
Clinical Trial
Molecular Weight

521.04

Formula

C28H37ClO7
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@](C[C@H](C)[C@]2(OC(CC)=O)C(COC(CC)=O)=O)([H])[C@]3([H])CCC4=CC(C=C[C@]4(C)[C@@]3(Cl)[C@@H](O)C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (191.92 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9192 mL 9.5962 mL 19.1924 mL
5 mM 0.3838 mL 1.9192 mL 3.8385 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.80 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (4.80 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.89%

COA (255 KB) LCMS (263 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9192 mL 9.5962 mL 19.1924 mL 47.9810 mL
5 mM 0.3838 mL 1.9192 mL 3.8385 mL 9.5962 mL
10 mM 0.1919 mL 0.9596 mL 1.9192 mL 4.7981 mL
15 mM 0.1279 mL 0.6397 mL 1.2795 mL 3.1987 mL
20 mM 0.0960 mL 0.4798 mL 0.9596 mL 2.3990 mL
25 mM 0.0768 mL 0.3838 mL 0.7677 mL 1.9192 mL
30 mM 0.0640 mL 0.3199 mL 0.6397 mL 1.5994 mL
40 mM 0.0480 mL 0.2399 mL 0.4798 mL 1.1995 mL
50 mM 0.0384 mL 0.1919 mL 0.3838 mL 0.9596 mL
60 mM 0.0320 mL 0.1599 mL 0.3199 mL 0.7997 mL
80 mM 0.0240 mL 0.1200 mL 0.2399 mL 0.5998 mL
100 mM 0.0192 mL 0.0960 mL 0.1919 mL 0.4798 mL
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Beclometasone dipropionate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Beclometasone5534-09-8Beclomethasone dipropionate Glucocorticoid ReceptorReactive Oxygen SpeciesNO SynthaseNitric oxide synthasesNOSSTAT-1iNOS16HBE cellsasthmainflammationInhibitorinhibitorinhibit

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