1. Apoptosis
  2. TNF-alpha

C 87 

Cat. No.: HY-100735 Purity: >98.0%
Data Sheet SDS Handling Instructions

C87 is a novel small-molecule TNFα inhibitor; potently inhibits TNFα-induced cytotoxicity with an IC50 of 8.73 μM.

For research use only. We do not sell to patients.
C 87 Chemical Structure

C 87 Chemical Structure

CAS No. : 332420-90-3

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO $111 In-stock
5 mg $100 In-stock
10 mg $150 In-stock
25 mg $310 In-stock
50 mg $558 In-stock
100 mg $998 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

C87 is a novel small-molecule TNFα inhibitor; potently inhibits TNFα-induced cytotoxicity with an IC50 of 8.73 μM.

IC50 & Target

IC50: 8.73 μM (TNFα-induced cytotoxicity)[1]

In Vitro

C87 directly binds to TNFα, potently inhibits TNFα-induced cytotoxicity (IC50=8.73 μM) and effectively blocks TNFα-triggered signaling activities. C87 exhibits good solubility and consistent dose-dependent functions in vitro. C87 completely blocks TNFα-induced activation of caspase-3 and caspase-8. The activity of c-Jun N-terminal kinase (JNK) is significantly reduced by C87 in L929 cells. C87 also prevents the degradation of IκBα in cells treated with TNFα. C87 potently blocks multiple signaling transduction pathways and downstream target gene activation triggered by TNFα[1].

In Vivo

C87 attenuates TNFα-induced inflammation, thereby markedly reducing injuries to the liver and improving animal survival. C87 injection delays the incidence of death and increases the survival rate by two folds compared with the vehicle control. The level of alanine transaminase and aspartate transaminase is consistently reduced in mice with C87 treatment[1].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.9883 mL 9.9417 mL 19.8835 mL
5 mM 0.3977 mL 1.9883 mL 3.9767 mL
10 mM 0.1988 mL 0.9942 mL 1.9883 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[1]

The inhibition of the cytotoxic effect of human TNFα by compounds (C87) is measured in L929 cell line. L929 cells are seeded at a density of 104 cells/well in a 96-well plate and incubated for 24 h in RPMI 1640 with 10% FBS at 37 °C. Compounds (C87) are added to the cells with 1 μg/mL actinomycin D and 1 ng/mL TNFα and then incubated at 37 °C for 20 h before analysis. Cell survival is measured using the MTT method[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

C 87 is prepared in DMSO.

BALB/c mice are used in the study. Before LPS/GalN challenge (LPS (50 μg/kg) and D-GalN (1.2g/kg)), mice are injected intraperitoneally with C87 (12.5 mg/kg), Enbrel (4 mg/kg), or vehicle, respectively, at 1, 8, and 16 h. Blood is collected with retro-orbital sampling. Activities of alanine transaminase and aspartate transaminase are detected. Liver tissues are collected and fixed in 10% formalin, and sections are stained with hematoxylin and eosin[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

502.93

Formula

C₂₄H₁₅ClN₆O₃S

CAS No.

332420-90-3

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: 9.33 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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C 87
Cat. No.:
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