1. Apoptosis
  2. TNF Receptor
  3. C 87

C 87 

Cat. No.: HY-100735 Purity: 98.07%
Handling Instructions

C 87 is a novel small-molecule TNFα inhibitor; potently inhibits TNFα-induced cytotoxicity with an IC50 of 8.73 μM.

For research use only. We do not sell to patients.

C 87 Chemical Structure

C 87 Chemical Structure

CAS No. : 332420-90-3

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Solution
10 mM * 1 mL in DMSO USD 133 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 133 In-stock
Estimated Time of Arrival: December 31
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5 mg USD 120 In-stock
Estimated Time of Arrival: December 31
10 mg USD 180 In-stock
Estimated Time of Arrival: December 31
25 mg USD 372 In-stock
Estimated Time of Arrival: December 31
50 mg USD 670 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1198 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

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Description

C 87 is a novel small-molecule TNFα inhibitor; potently inhibits TNFα-induced cytotoxicity with an IC50 of 8.73 μM.

IC50 & Target

IC50: 8.73 μM (TNFα-induced cytotoxicity)[1]

In Vitro

C 87 (C87) directly binds to TNFα, potently inhibits TNFα-induced cytotoxicity (IC50=8.73 μM) and effectively blocks TNFα-triggered signaling activities. C 87 exhibits good solubility and consistent dose-dependent functions in vitro. C 87 completely blocks TNFα-induced activation of caspase-3 and caspase-8. The activity of c-Jun N-terminal kinase (JNK) is significantly reduced by C 87 in L929 cells. C 87 also prevents the degradation of IκBα in cells treated with TNFα. C 87 potently blocks multiple signaling transduction pathways and downstream target gene activation triggered by TNFα[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

C 87 (C87) attenuates TNFα-induced inflammation, thereby markedly reducing injuries to the liver and improving animal survival. C 87 injection delays the incidence of death and increases the survival rate by two folds compared with the vehicle control. The level of alanine transaminase and aspartate transaminase is consistently reduced in mice with C 87 treatment[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

502.93

Formula

C₂₄H₁₅ClN₆O₃S

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 9.33 mg/mL (18.55 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9883 mL 9.9417 mL 19.8835 mL
5 mM 0.3977 mL 1.9883 mL 3.9767 mL
10 mM 0.1988 mL 0.9942 mL 1.9883 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

The inhibition of the cytotoxic effect of human TNFα by compounds (C 87) is measured in L929 cell line. L929 cells are seeded at a density of 104 cells/well in a 96-well plate and incubated for 24 h in RPMI 1640 with 10% FBS at 37°C. Compounds (C 87) are added to the cells with 1 μg/mL actinomycin D and 1 ng/mL TNFα and then incubated at 37 °C for 20 h before analysis. Cell survival is measured using the MTT method[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

BALB/c mice are used in the study. Before LPS/GalN challenge (LPS (50 μg/kg) and D-GalN (1.2g/kg)), mice are injected intraperitoneally with C 87 (12.5 mg/kg), Enbrel (4 mg/kg), or vehicle, respectively, at 1, 8, and 16 h. Blood is collected with retro-orbital sampling. Activities of alanine transaminase and aspartate transaminase are detected. Liver tissues are collected and fixed in 10% formalin, and sections are stained with hematoxylin and eosin[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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C 87
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HY-100735
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