1. Autophagy
  2. Autophagy

CA-5f 

Cat. No.: HY-112698 Purity: 99.12%
Handling Instructions

CA-5f is a potent late-stage macroautophagy/autophagy inhibitor via inhibiting autophagosome-lysosome fusion. CA-5f increases LC3B-II (a marker to monitor autophagy) and SQSTM1 protein both in A549 cells and HUVECs. Anti-tumor activity.

For research use only. We do not sell to patients.

CA-5f Chemical Structure

CA-5f Chemical Structure

CAS No. : 1370032-19-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 121 In-stock
Estimated Time of Arrival: December 31
5 mg USD 110 In-stock
Estimated Time of Arrival: December 31
10 mg USD 180 In-stock
Estimated Time of Arrival: December 31
25 mg USD 380 In-stock
Estimated Time of Arrival: December 31
50 mg USD 680 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1100 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Description

CA-5f is a potent late-stage macroautophagy/autophagy inhibitor via inhibiting autophagosome-lysosome fusion. CA-5f increases LC3B-II (a marker to monitor autophagy) and SQSTM1 protein both in A549 cells and HUVECs. Anti-tumor activity[1].

IC50 & Target

Macroautophagy/autophagy[1]

In Vitro

CA-5f (0-40 μM, 6 hour) concentration- and time-dependently elevates the level of LC3B-II (a marker to monitor autophagy) and SQSTM1 protein both in A549 cells and HUVECs[1].
CA-5f (20 μM, 6 hours) inhibits the degradation of autophagosomes when treated alone or in combination Bafilomycin A1 (100 nM) or Chloroquine (30 μM) in A549 cells and HUVECs[1].
CA-5f (20 μM) neither impairs the hydrolytic function nor the quantity of lysosomes[1].
CA-5f (20 μM, 96 hours) inhibits the growth of A549 cells, and less cytotoxic to normal HUVECs[1].

Cell Viability Assay[1]

Cell Line: A549, HUVECs
Concentration: 20 μM
Incubation Time: 96 hours
Result: Exhibited more cytotoxicity against A549 cells compared with normal HUVECs.

Western Blot Analysis[1]

Cell Line: A549 cells and HUVECs
Concentration: 0-40 μM
Incubation Time: 6 hours
Result: Elevated LC3B-II (a marker to monitor autophagy) and SQSTM1 protein levels in a concentration- and time-dependent manner.
In Vivo

CA-5f (40 mg/kg, i.p., every 2 days for up to 30 days) is well tolerated, and potently inhibits the growth of tumor in nude mice bearing A549 lung cancer cells[1].
CA-5f (40 mg/kg, i.p.) suppresses autophagic flux and induces apoptosis in nude mice bearing A549 lung cancer cells[1].

Animal Model: Nude mice bearing A549 lung cancer cells[1]
Dosage: 40 mg/kg
Administration: Injected via caudal vein, every 2 days for up to 30 days
Result: Significantly suppressed tumor volume and weight in mice, increased the number of apoptotic cells in mice.
Solvent & Solubility
In Vitro: 

DMSO : 77.5 mg/mL (199.51 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5743 mL 12.8713 mL 25.7427 mL
5 mM 0.5149 mL 2.5743 mL 5.1485 mL
10 mM 0.2574 mL 1.2871 mL 2.5743 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (5.35 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.08 mg/mL (5.35 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (5.35 mM); Clear solution

References
Molecular Weight

388.46

Formula

C₂₄H₂₄N₂O₃

CAS No.

1370032-19-1

SMILES

O=C(/C(CN(C)C/1)=C/C2=CNC3=C2C=CC=C3)C1=C\C4=CC=C(OC)C(OC)=C4

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
CA-5f
Cat. No.:
HY-112698
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CA-5f

Cat. No.: HY-112698