2. Autophagy
  3. Autophagy
  4. DC-LC3in-D5

DC-LC3in-D5 

Cat. No.: HY-141882 Purity: 98.51%
COA Handling Instructions

DC-LC3in-D5 acts as an autophagy inhibitor by attenuating LC3B lipidation. DC-LC3in-D5 binds with LC3B. DC-LC3in-D5 disrupts the LC3B-LBP2 interaction with an IC50 of 200 nM. DC-LC3in-D5 may contribute to anti-HCV or combination researchs in cancer through inhibiting autophagy.

For research use only. We do not sell to patients.

DC-LC3in-D5 Chemical Structure

DC-LC3in-D5 Chemical Structure

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 330 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 330 In-stock
Solid
5 mg USD 300 In-stock
10 mg USD 480 In-stock
25 mg USD 980 In-stock
50 mg USD 1500 In-stock
100 mg USD 2350 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

DC-LC3in-D5 acts as an autophagy inhibitor by attenuating LC3B lipidation. DC-LC3in-D5 binds with LC3B. DC-LC3in-D5 disrupts the LC3B-LBP2 interaction with an IC50 of 200 nM. DC-LC3in-D5 may contribute to anti-HCV or combination researchs in cancer through inhibiting autophagy[1].

In Vitro

DC-LC3in-D5 demonstrates high selectivity to LC3A/B in the proteome. DC-LC3in-D5 exhibits a potent covalent reactivity and selectivity to LC3A/B in HeLa cells[1].
Treatment of HeLa cells with DC-LC3in-D5 (3-30 μM) results in disruption of LC3B lipidation, inhibition of autophagic vesicle formation, and accumulation of p62[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HeLa cells
Concentration: 3, 10, 30 μM
Incubation Time: 16 hours
Result: Pre-treated accumulated significant more p62 than DMSO-treated control samples. Attenuated LC3-I/II lipidation in cells exposed to autophagy inducing conditions.
Molecular Weight

397.30

Appearance

Solid

Formula

C19H22Cl2N2O3
SMILES

O=C1/C(C(CC(C2=C(Cl)C(Cl)=CC=C2)C1)=O)=C/NCCN3CCOCC3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 35.71 mg/mL (89.88 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5170 mL 12.5849 mL 25.1699 mL
5 mM 0.5034 mL 2.5170 mL 5.0340 mL
10 mM 0.2517 mL 1.2585 mL 2.5170 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

DC-LC3in-D5AutophagyLC3B lipidation ,LC3B-LBP2 interactionanti-HCVcancerInhibitorinhibitorinhibit

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