1. Vitamin D Related
    Metabolic Enzyme/Protease
  2. VD/VDR
    Endogenous Metabolite
  3. Vitamin D3

Vitamin D3 (Synonyms: Cholecalciferol; Colecalciferol)

Cat. No.: HY-15398 Purity: 99.94%
Handling Instructions

Vitamin D3 (Cholecalciferol; Colecalciferol) is a naturally occuring form of vitamin D. Vitamin D3 induces cell differentiation and prevents proliferation of cancer cells.

For research use only. We do not sell to patients.

Vitamin D3 Chemical Structure

Vitamin D3 Chemical Structure

CAS No. : 67-97-0

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Based on 1 publication(s) in Google Scholar

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Description

Vitamin D3 (Cholecalciferol; Colecalciferol) is a naturally occuring form of vitamin D. Vitamin D3 induces cell differentiation and prevents proliferation of cancer cells.

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Vitamin D3 is an inactive vitamin D molecule in vivo. Vitamin D3 undergoes two hydroxylation processes to activate it. Vitamin D3 is first hydroxylated in the liver to form the circulating prohormone 25-hydroxy vitamin D3 [25(OH)D3] by the enzyme 25-hydroxylase (CYP27A1) and probably also by other enzymes (e.g., CYP2R1)[1].
The second hydroxylation occurs in the kidneys via the enzyme 1-alpha-hydroxylase, yielding 1,25- dihydroxycholecalciferol (calcitriol), which is the biologically active form of vitamin D[1].
Vitamin D3 (2-10 μM; 24-48 hours) exhibits anti-proliferative effects in a dose- and time- dependent manner. Maximal reduction of viability post treatment of 62% (IK), 52% (RL-95-2), and 55% (Hec-1A) occurrs by 72 h of treatment with 10 μM Vitamin D3. but 24-hour exposure lacks significant reduction in viable cells[2].
Cholecalciferol (10 μM; 24-48 hours) shows marked increases in nuclear VDR staining and produces local VDR activation in IK cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: EC cell lines from EEC, Ishikawa 3-H-12(IK), RL-95/2, and HEC-1-A cells
Concentration: 2-10 μM
Incubation Time: 24-72 hours
Result: Reduced viability in response to VD3 in a dose- and time-dependent manner.
Indicated that the conversion of VD3 to 25(OH)D is an essential step for the reduced cell viability effect.

Cell Viability Assay[2]

Cell Line: EC cell lines from EEC, Ishikawa 3-H-12(IK) cells
Concentration: 10 μM
Incubation Time: 24-48 hours
Result: Improved nuclear VDR content in IK cells.
In Vivo

Cholecalciferol (oral gavage; 5 mg/kg; 7 days) potentiates the CCl4 toxicity only in the liver, as indicated by plasma levels of ALT and AST, biochemical markers of hepatic damage. It significantly increases renal calcium levels in mice, but renal calcium content does not differ significantly between mice[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male ddY mice on CCl4 toxicity[3]
Dosage: 5 mg/kg
Administration: Oral gavage; 5 mg/kg; 7 days
Result: Potentiated CCl4-induced hepatotoxicity and enhanced mouse mortality, without increasing renal toxicity and generation of liver fibrosis.
Clinical Trial
Molecular Weight

384.64

Formula

C₂₇H₄₄O

CAS No.

67-97-0

SMILES

C[[email protected]@]12[[email protected]](CC[[email protected]]2([H])[[email protected]](C)CCCC(C)C)([H])/C(CCC1)=C/C=C3C(CC[[email protected]](O)C\3)=C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (259.98 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5998 mL 12.9992 mL 25.9983 mL
5 mM 0.5200 mL 2.5998 mL 5.1997 mL
10 mM 0.2600 mL 1.2999 mL 2.5998 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (5.41 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.08 mg/mL (5.41 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (5.41 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: 99.94%

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Keywords:

Vitamin D3Cholecalciferol ColecalciferolVitamin D 3Vitamin D-3VD/VDREndogenous MetaboliteVitamin DVitamin D receptorHypertensionType I DiabeteCardiovascular DiseaseCancermetastaticfatalcalciumabsorbInhibitorinhibitorinhibit

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