Vitamin D3 (Synonyms: Cholecalciferol; Colecalciferol)

Name: Vitamin D3
Brand: MedChemExpress
SKU: HY-15398
Rating: 5.0 (22 reviews)

Cat. No.: HY-15398 Purity: 99.94%: Data Sheet
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Vitamin D3 (Cholecalciferol; Colecalciferol) is a naturally occuring form of vitamin D. Vitamin D3 induces cell differentiation and prevents proliferation of cancer cells.

For research use only. We do not sell to patients.

CAS No. : 67-97-0

Size	Stock	Quantity
100 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 22 publication(s) in Google Scholar

Other Forms of Vitamin D3:

22 Publications Citing Use of MCE Vitamin D3

In Vivo Efficacy Study

IHC

RT-PCR

: Vitamin D3 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Nov 9;81(1):448. [Abstract]; Levels of calcium content and ALP activity in aortic tissues from Vitamin D3 (VitD3; 5×10⁵ IU/kg; subcutaneous injection; three consecutive days)-overloaded mice.

: Vitamin D3 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Nov 9;81(1):448. [Abstract]; SIRT1, HIF-1α, RUNX2, BMP2, α-SMA, and SM22α protein levels in the aortic tissues from Vitamin D3 (VitD3; 5×10⁵ IU/kg; subcutaneous injection; three consecutive days)-overloaded mice were analyzed via Western immunoblotting.

: Vitamin D3 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Nov 9;81(1):448. [Abstract]; RUNX2, BMP2, α-SMA, and SM22α mRNA levels were analyzed in aortic tissues from Vitamin D3 (VitD3; 5×10⁵ IU/kg; subcutaneous injection; three consecutive days)-overloaded mice.

: Vitamin D3 purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jan 1;14(1):436-450. [Abstract]; Representative in vivo images of MHCC-97H-luc-tumor-bearing-BALB/c-nude mice with Vitamin D3 treatment (1500 IU/kg dissolved in absolute ethanol to the daily drinking water; continuously for 2 months) and quantified valuesare shown as relative luciferase activity.

: Vitamin D3 purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jan 1;14(1):436-450. [Abstract]; Representative images of CD31-PAS double staining, HE staining, IHC staining of VDBP, VE-cadherin, E-cadherin, MMP2 and MMP9 and PLA assays in liver tissues of MHCC-97H-luc-tumor-bearing-BALB/c-nude mice with Vitamin D3 treatment (1500 IU/kg dissolved in absolute ethanol to the daily drinking water; continuously for 2 months) .

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Description

Vitamin D3 (Cholecalciferol; Colecalciferol) is a naturally occuring form of vitamin D. Vitamin D3 induces cell differentiation and prevents proliferation of cancer cells.

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
C3H 10T1/2	IC₅₀	2.9 μM Compound: 3, VD3	Inhibition of Hedgehog signaling in mouse C3H10T1/2 cells assessed as downregulation of Ptch mRNA expression after 24 hrs by RT-PCR analysis Inhibition of Hedgehog signaling in mouse C3H10T1/2 cells assessed as downregulation of Ptch mRNA expression after 24 hrs by RT-PCR analysis	[PMID: 24900716]
C3H 10T1/2	IC₅₀	2.9 μM Compound: 3, VD3, Vitamin D3	Inhibition of hedgehog signaling pathway-mediated PTCH mRNA expression in mouse C3H10T1/2 cells after 24 hrs by RT-PCR analysis Inhibition of hedgehog signaling pathway-mediated PTCH mRNA expression in mouse C3H10T1/2 cells after 24 hrs by RT-PCR analysis	[PMID: 24730984]
C3H 10T1/2	IC₅₀	4.1 μM Compound: 2, VD3, vitamin D3	Inhibition of hedgehog signaling in mouse C3H10T1/2 cells assessed as reduction in Gli1 mRNA level after 24 hrs by quantitative PCR analysis Inhibition of hedgehog signaling in mouse C3H10T1/2 cells assessed as reduction in Gli1 mRNA level after 24 hrs by quantitative PCR analysis	[PMID: 25676864]
C3H 10T1/2	IC₅₀	4.1 μM Compound: 3, VD3	Inhibition of Hedgehog signaling in mouse C3H10T1/2 cells assessed as downregulation of Gli1 mRNA expression after 24 hrs by RT-PCR analysis Inhibition of Hedgehog signaling in mouse C3H10T1/2 cells assessed as downregulation of Gli1 mRNA expression after 24 hrs by RT-PCR analysis	[PMID: 24900716]
C3H 10T1/2	IC₅₀	4.1 μM Compound: 3, VD3, Vitamin D3	Inhibition of hedgehog signaling pathway-mediated GLI1 mRNA expression in mouse C3H10T1/2 cells after 24 hrs by RT-PCR analysis Inhibition of hedgehog signaling pathway-mediated GLI1 mRNA expression in mouse C3H10T1/2 cells after 24 hrs by RT-PCR analysis	[PMID: 24730984]
Daoy	IC₅₀	> 10 μM Compound: 3, VD3	Inhibition of Hedgehog signaling in human DaOY cells assessed as downregulation of Gli1 mRNA expression after 48 hrs by RT-PCR analysis Inhibition of Hedgehog signaling in human DaOY cells assessed as downregulation of Gli1 mRNA expression after 48 hrs by RT-PCR analysis	[PMID: 24900716]
Daoy	IC₅₀	> 10 μM Compound: 3, VD3, Vitamin D3	Inhibition of hedgehog signaling pathway-mediated GLI1 mRNA expression in human DaOY cells after 24 hrs by RT-PCR analysis Inhibition of hedgehog signaling pathway-mediated GLI1 mRNA expression in human DaOY cells after 24 hrs by RT-PCR analysis	[PMID: 24730984]
Daoy	IC₅₀	> 10 μM Compound: 3, VD3, Vitamin D3	Inhibition of hedgehog signaling pathway-mediated PTCH mRNA expression in human DaOY cells after 24 hrs by RT-PCR analysis Inhibition of hedgehog signaling pathway-mediated PTCH mRNA expression in human DaOY cells after 24 hrs by RT-PCR analysis	[PMID: 24730984]
HEK293	EC₅₀	0.21 nM Compound: VitD3	Agonist activity at human VDR expressed in HEK293 cells by luciferase reporter gene assay Agonist activity at human VDR expressed in HEK293 cells by luciferase reporter gene assay	[PMID: 25305688]
HT-29	GI₅₀	53.8 μM Compound: 2, VD3	Growth inhibition of human HT-29 cells after 72 hrs by MTS-PMS assay Growth inhibition of human HT-29 cells after 72 hrs by MTS-PMS assay	[PMID: 23972439]
HT-29	GI₅₀	68.5 μM Compound: VD3	Growth inhibition of human HT-29 cells overexpressing VDR gene after 72 hrs by MTS assay Growth inhibition of human HT-29 cells overexpressing VDR gene after 72 hrs by MTS assay	[PMID: 22687748]
Medulloblastoma cell	GI₅₀	5.8 μM Compound: 1; VD3	Antiproliferative activity against medulloblastoma cells derived from conditional patched knockout mouse after 48 hrs by [3H]-thymidine incorporation assay Antiproliferative activity against medulloblastoma cells derived from conditional patched knockout mouse after 48 hrs by [3H]-thymidine incorporation assay	[PMID: 30471551]
U-87MG ATCC	GI₅₀	23.2 μM Compound: VD3	Growth inhibition of human U87MG cells after 72 hrs by MTS assay Growth inhibition of human U87MG cells after 72 hrs by MTS assay	[PMID: 22687748]
U-87MG ATCC	IC₅₀	29.7 μM Compound: VD3	Antiproliferative activity against human U87MG cells after 72 hrs by MTS assay Antiproliferative activity against human U87MG cells after 72 hrs by MTS assay	[PMID: 22226657]

In Vitro

Vitamin D3 is an inactive vitamin D molecule in vivo. Vitamin D3 undergoes two hydroxylation processes to activate it. Vitamin D3 is first hydroxylated in the liver to form the circulating prohormone 25-hydroxy vitamin D3 [25(OH)D3] by the enzyme 25-hydroxylase (CYP27A1) and probably also by other enzymes (e.g., CYP2R1)^[1].
The second hydroxylation occurs in the kidneys via the enzyme 1-alpha-hydroxylase, yielding 1,25- dihydroxycholecalciferol (calcitriol), which is the biologically active form of vitamin D^[1].
Vitamin D3 (2-10 μM; 24-48 hours) exhibits anti-proliferative effects in a dose- and time-dependent manner. Maximal reduction of viability post-treatment of 62% (IK), 52% (RL-95-2), and 55% (Hec-1A) occurs by 72 h of treatment with 10 μM Vitamin D3. but 24-hour exposure lacks significant reduction in viable cells^[2].
Cholecalciferol (10 μM; 24-48 hours) shows marked increases in nuclear VDR staining and produces local VDR activation in IK cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	EC cell lines from EEC, Ishikawa 3-H-12(IK), RL-95/2, and HEC-1-A cells
Concentration:	2-10 μM
Incubation Time:	24-72 hours
Result:	Reduced viability in response to VD3 in a dose- and time-dependent manner. Indicated that the conversion of VD3 to 25(OH)D is an essential step for the reduced cell viability effect.

Cell Viability Assay^[2]

Cell Line:	EC cell lines from EEC, Ishikawa 3-H-12(IK) cells
Concentration:	10 μM
Incubation Time:	24-48 hours
Result:	Improved nuclear VDR content in IK cells.

In Vivo

Cholecalciferol (oral gavage; 5 mg/kg; 7 days) potentiates the CCl4 toxicity only in the liver, as indicated by plasma levels of ALT and AST, biochemical markers of hepatic damage. It significantly increases renal calcium levels in mice, but renal calcium content does not differ significantly between mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male ddY mice on CCl4 toxicity^[3]
Dosage:	5 mg/kg
Administration:	Oral gavage; 5 mg/kg; 7 days
Result:	Potentiated CCl4-induced hepatotoxicity and enhanced mouse mortality, without increasing renal toxicity and generation of liver fibrosis.

Clinical Trial

Molecular Weight

384.64

Formula

C₂₇H₄₄O

CAS No.

67-97-0

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCCC(C)C)([H])/C(CCC1)=C/C=C3C(CC[C@@H](C\3)O)=C

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (26.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5998 mL	12.9992 mL	25.9983 mL
5 mM	0.5200 mL	2.5998 mL	5.1997 mL
10 mM	0.2600 mL	1.2999 mL	2.5998 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (5.41 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (2.60 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1 mg/mL (2.60 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (277 KB) SDS (584 KB)

COA (247 KB) HNMR (291 KB) RP-HPLC (184 KB)

Handling Instructions (2659 KB)

References

[1]. Nazik Al-Hashimi, et al. Cholecalciferol

[2]. Laura Bergadà, et al. Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of cell growth in normal endometrium and endometrial carcinoma. Lab Invest. 2014 Jun;94(6):608-22 [Content Brief]

[3]. Hiroki Yoshioka, et al. Vitamin D3-induced hypercalcemia increases carbon tetrachloride-induced hepatotoxicity through elevated oxidative stress in mice. PLoS One. 2017 Apr 27;12(4):e0176524. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5998 mL	12.9992 mL	25.9983 mL	64.9958 mL
	5 mM	0.5200 mL	2.5998 mL	5.1997 mL	12.9992 mL
	10 mM	0.2600 mL	1.2999 mL	2.5998 mL	6.4996 mL
	15 mM	0.1733 mL	0.8666 mL	1.7332 mL	4.3331 mL
	20 mM	0.1300 mL	0.6500 mL	1.2999 mL	3.2498 mL
	25 mM	0.1040 mL	0.5200 mL	1.0399 mL	2.5998 mL

Vitamin D3 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Vitamin D3 (Synonyms: Cholecalciferol; Colecalciferol)

Customer Review

Other Forms of Vitamin D3:

Vitamin D3 Related Antibodies

22 Publications Citing Use of MCE Vitamin D3

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•Related Screening Libraries:

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View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Vitamin D3 Related Antibodies

Molarity Calculator

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Complete Stock Solution Preparation Table

Vitamin D3 Related Classifications

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