Cat. No.: HY-17459

Clopidogrel hydrogen sulfate

(Synonyms: (S)-(+)-Clopidogrel bisulfate; (S)-(+)-Clopidogrel hydrogen sulfate)

32 Cited Publications

Customer Review

Based on 32 publication(s) in Google Scholar

Clopidogrel hydrogen sulfate is an antiplatelet agent to prevent blood clots. Clopidogrel hydrogen sulfate inhibits CYP2B6 and CYP2C19 with IC₅₀s of 18.2 nM and 524 nM, respectively. Clopidogrel hydrogen sulfate is a potent antithrombotic agent that inhibits ADP-induced platelet aggregation．Clopidogrel hydrogen sulfate also is an orally active P2Y(12) inhibitor.

For research use only. We do not sell to patients.

Purity: 99.88%
CAS No.: 120202-66-6
Formula: C₁₆H₁₈ClNO₆S₂
Molecular Weight:419.90

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Clopidogrel hydrogen sulfate Chemical Structure

CAS No.: 120202-66-6

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (32)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Clopidogrel hydrogen sulfate is an antiplatelet agent to prevent blood clots. Clopidogrel hydrogen sulfate inhibits CYP2B6 and CYP2C19 with IC₅₀s of 18.2 nM and 524 nM, respectively^[1]. Clopidogrel hydrogen sulfate is a potent antithrombotic agent that inhibits ADP-induced platelet aggregation^[2]．Clopidogrel hydrogen sulfate also is an orally active P2Y(12) inhibitor^[5].

IC₅₀ & Target^[1]

CYP2B6

18.2 nM (IC₅₀)

P2Y12 Receptor

CYP2C19

524 nM (IC₅₀)

All Cytochrome P450 Isoforms :

All P2Y Receptor Isoforms :

In Vitro

Clopidogrel (1.5 mM;12 and 24 hours) up-regulates the expression of TRIB3 and CHOP in a concentration- and time-dependent manner, as measured by real-time PCR and Western blot analysis^[3].
Clopidogrel (1.5 mM; 24 hours) significantly induces human gastric epithelial cell (GES-1) apoptosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:

GES-1 cells
Concentration:

1.5 mM
Incubation Time:

12 and 24 hours
Result:

The mRNA expression levels of both CHOP and TRIB3 were up-regulated in a concentration- and time-dependent manner.
The protein expression levels of both CHOP and TRIB3 were up-regulated in a concentration- and time-dependent manner.

Apoptosis Analysis^[3]

Cell Line:

Gastric epithelial cell (GES-1) cells
Concentration:

1.5 mM
Incubation Time:

24 hours
Result:

Induced apoptosis of gastric epithelial cells．

In Vivo

Clopidogrel (5 mg/kg) significantly inhibits thrombus formation compared with vehicle. Acetylsalicylic acid (0.15 mg/kg) can add to the antithrombotic effect of Clopidogrel in mice; Acetylsalicylic acid (0.6 mg/kg) blunts the antithrombotic effect of Clopidogrel^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Age-matched C57BL/6J male mice weighing at least 25 g were used at 8 to 12 weeks of age^[4]
Dosage:

5 mg/kg
Administration:

Gavage treatment
Result:

Clopidogrel significantly inhibited thrombus formation compared with vehicle.
When Clopidogrel was given in combination with 0.6 mg/kg ASA, thrombi were significantly larger than those measured with Clopidogrel alone.
In contrast, when Clopidogrel was given in combination with 0.15 mg/kg ASA , thrombi were significantly smaller than those in mice treated with Clopidogrel and 0.6 mg/kg ASA, and smaller than those in mice given Clopidogrel alone.

Chemical Information

CAS No. 120202-66-6
Appearance Solid
Molecular Weight 419.90
Formula C₁₆H₁₈ClNO₆S₂
Color Off-white to light yellow
SMILES

ClC1=C([C@@H](C(OC)=O)N2CCC3=C(C=CS3)C2)C=CC=C1.O=S(O)(O)=O
Synonyms

(S)-(+)-Clopidogrel bisulfate; (S)-(+)-Clopidogrel hydrogen sulfate
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (32)

All Cell Imaging/StainingHistological Imaging/StainingRT-PCRWBIn Vivo Efficacy Study

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Journal Impact Factor

ACS Nano

Cryo-Shocked Platelet Coupled with ROS-Responsive Nanomedicine for Targeted Treatment of Thromboembolic Disease. [Abstract]

2023 Apr 11;17(7):6519-6533. PMID: 36971410
J Adv Res

Targeting MAD2B as a strategy for ischemic stroke therapy. [Abstract]

2025 Jun:72:369-385. PMID: 38972542
Neuron

More than microglial depletion: PLX5622 activates the hepatic constitutive androstane receptor to alter anesthesia and addiction. [Abstract]

2026 Apr 15;114(8):1419-1432.e7. PMID: 41734759
Theranostics

Platelets promote CRC by activating the C5a/C5aR1 axis via PSGL-1/JNK/STAT1 signaling in tumor-associated macrophages. [Abstract]

2023 Mar 27;13(6):2040-2056. PMID: 37064877
Int J Biol Sci

Clopidogrel Reduces Fibronectin Accumulation and Improves Diabetes-Induced Renal Fibrosis. [Abstract]

2019 Jan 1;15(1):239-252. PMID: 30662363

Clopidogrel hydrogen sulfate purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2019 Jan 1;15(1):239-252. [Abstract]

Representative images of kidney tissue H&E (upper panel) and Sirius red staining (lower panel) of control (Ctl) and diabetic mice (DM) with or without Clopidogrel (20 mg/kg, p.o.) treatment (Ctl + Clo & DM + Clo).

Clopidogrel hydrogen sulfate purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2019 Jan 1;15(1):239-252. [Abstract]

RT-PCR analysis of the T1D kidney tissue showed significantly elevated mRNA levels of ECM molecules, such as FN and collagen I, which were reduced by the Clopidogrel (20 mg/kg, p.o.) treatment.

Clopidogrel hydrogen sulfate purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2019 Jan 1;15(1):239-252. [Abstract]

Western blot analysis confirmed that the T1D-induced increase in the FN expression at six months was decreased by the Clopidogrel (20 mg/kg, p.o.) treatment.

Clopidogrel hydrogen sulfate purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2019 Jan 1;15(1):239-252. [Abstract]

The Clopidogrel (20 mg/kg, p.o.) treatment did not affect the body weight, however, it significantly decreased the blood glucose.
J Neuroinflammation

Hyperglycemia impairs microglia responding to retinal vasculopathy via enhanced norepinephrine-ADRB2 signaling. [Abstract]

2025 Dec 8. PMID: 41361454

Clopidogrel hydrogen sulfate purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2025 Dec 8. [Abstract]

In vehicle-treated retinas, microglial processes rapidly converged toward the injury site within 30 min. This response was markedly suppressed by Clopidogrel (10 mg/kg, i.p.) treatment.
ACS Environ Au

Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]

2025 Aug 5;5(6):573-582. PMID: 41277996
Int J Mol Med

Platelet activation stimulates macrophages to enhance ulcerative colitis through PF4/CXCR3 signaling. [Abstract]

2025 May;55(5):78. PMID: 40084691
PLoS Biol

Microglia sense fungal infections through capsular components from capillary-bound Cryptococcus neoformans via endothelial nucleotide signaling. [Abstract]

2026 Feb 6;24(2):e3003642. PMID: 41650189
J Ethnopharmacol

Mailuoning oral liquid ameliorates vasculitis in thromboangiitis obliterans rats via inactivating cGAS-STING-IRF3 and TLR4-MAPKs/NF-κB signaling pathways. [Abstract]

2025 Jan 30;337(Pt 1):118707. PMID: 39181282
Biochem Pharmacol

Tyrphostin AG538 inhibits platelet activation and thrombosis via TREM2 agonism. [Abstract]

2025 Dec;242(Pt 3):117379. PMID: 41046073
Drug Des Devel Ther

Clopidogrel-Induced Gastric Injury in Rats is Attenuated by Stable Gastric Pentadecapeptide BPC 157. [Abstract]

2020 Dec 21:14:5599-5610. PMID: 33376304
Foods

Structural Insights on Hyp-Gly-Containing Peptides as Antiplatelet Compounds through Topomer CoMFA and CoMSIA Analysis. [Abstract]

2023 Feb 10;12(4):777. PMID: 36832851
Int J Mol Sci

Rescue of Mitochondrial SIRT3 Ameliorates Ischemia-like Injury in Human Endothelial Cells. [Abstract]

2022 Aug 14;23(16):9118. PMID: 36012382
Front Pharmacol

Clopidogrel ameliorates high-fat diet-induced hepatic steatosis in mice through activation of the AMPK signaling pathway and beyond. [Abstract]

2024 Oct 23:15:1496639. PMID: 39508046
Front Pharmacol

Hsp47 Inhibitor Col003 Attenuates Collagen-Induced Platelet Activation and Cerebral Ischemic-Reperfusion Injury in Rats. [Abstract]

2022 Jan 10;12:792263. PMID: 35082674
Front Pharmacol

Berberine and Its Main Metabolite Berberrubine Inhibit Platelet Activation Through Suppressing the Class I PI3Kβ/Rasa3/Rap1 Pathway. [Abstract]

2021 Oct 8:12:734603. PMID: 34690771
ACS Omega

Purification and Characterization of Novel Collagen Peptides against Platelet Aggregation and Thrombosis from Salmo salar. [Abstract]

2020 Aug 4;5(32):19995-20003. PMID: 32832753
iScience

The mechanisms and therapeutic potential of clopidogrel in mitigating diabetic cardiomyopathy in db/db mice. [Abstract]

2024 Feb 5;27(3):109134. PMID: 38375215
Clin Chim Acta

Simultaneous quantification of acetylsalicylic acid, clopidogrel, ticagrelor and their major metabolites in human plasma by liquid chromatography-tandem mass spectrometry. [Abstract]

2025 Dec 31:120816. PMID: 41482259
J Cell Physiol

Distinct Thromboxane A₂-Dependent Pathways Regulate Arachidonic Acid-Triggered VASP Phosphorylation at Ser239 and Ser157 in Human Platelets: Real-Time Visualization Reveals Superior Antithrombotic Efficacy by Targeting Thromboxane A₂ Signaling over Cyclooxygenase Inhibition. [Abstract]

2026 Mar;241(3):e70160. PMID: 41800570
J Appl Physiol (1985)

Intravital assessment of precapillary pulmonary arterioles of type 1 diabetic mice shows oxidative damage and increased tone in response to NOS inhibition. [Abstract]

2021 Nov 1;131(5):1552-1564. PMID: 34590907
Thromb Res

WY-14643, a novel antiplatelet and antithrombotic agent targeting the GPIbα receptor. [Abstract]

2024 Jun:238:41-51. PMID: 38669962
Thromb Res

OKL-1111, A modified cyclodextrin as a potential universal reversal agent for anticoagulants. [Abstract]

2023 Jul:227:17-24. PMID: 37207560
Neuroscience

P2Y12 receptor in trigeminal ganglion contributes to CFA-induced mechanical allodynia in mice. [Abstract]

2026 Aug 17:609:156-168. PMID: 42177012
Thromb J

Integrated proteomic and metabolomic profiling of lymph after trauma-induced hypercoagulopathy and antithrombotic therapy. [Abstract]

2024 Jul 10;22(1):59. PMID: 38987792
Open Chem

2020 Jun.
J Food Biochem

A novel di-peptide Met-Glu from collagen hydrolysates inhibits platelet aggregation and thrombus formation via regulation of Gq-mediated signaling. [Abstract]

2020 Sep;44(9):e13352. PMID: 32662128
Am J Transl Res

Role of MKP-5-p38/MAPK pathway in Clopidogrel-induced gastric mucosal epithelial cells apoptosis and tight junction dysfunction. [Abstract]

2020 May 15;12(5):1741-1753. PMID: 32509173

Clopidogrel hydrogen sulfate purchased from MedChemExpress. Usage Cited in: Am J Transl Res. 2020 May 15;12(5):1741-1753. [Abstract]

Expression of MKP-5 and phosphorylated p38/MAPK in Clopidogrel-treated gastric mucosal tissues and cells. Western blot analysis show MKP-5 is decreased and negatively correlated of Clopidogrel.
J Pharm Health Care Sci

Mixture of clopidogrel bisulfate and magnesium oxide tablets reduces clopidogrel dose administered through a feeding tube. [Abstract]

2021 May 4;7(1):18. PMID: 33941283
University of Colorado Denver

2024.
Res Sq

Lymphatic platelet thrombosis limits bone repair by precluding lymphatic transporting DAMPs. [Abstract]

2023 Nov 14:rs.3.rs-3474507. PMID: 38014223

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (476.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 16.67 mg/mL (39.70 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3815 mL

11.9076 mL

23.8152 mL

5 mM

0.4763 mL

2.3815 mL

4.7630 mL

10 mM

0.2382 mL

1.1908 mL

2.3815 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 5 mg/mL (11.91 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 5 mg/mL (11.91 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (11.91 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 50 mg/mL (119.08 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (621 KB)
- English - EN (621 KB)
- Français - FR (621 KB)
- Deutsch - DE (621 KB)
- Norwegian - NO (621 KB)
- Español - ES (621 KB)
- Swedish - SV (621 KB)
- Italian - IT (621 KB)
- Korean - KR (621 KB)
- Portuguese - PT (621 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Katsunobu Hagihara, et al. Comparison of human cytochrome P450 inhibition by the thienopyridines prasugrel, Clopidogrel, and ticlopidine. Drug Metab Pharmacokinet. 2008;23(6):412-20. [Content Brief]

[2]. Jean-Marc Herbert, et al. P2Y12, a new platelet ADP receptor, target of Clopidogrel.Semin Vasc Med. 2003 May;3(2):113-22. [Content Brief]

[3]. Hai-Lu Wu, et al. Increased endoplasmic reticulum stress response is involved in Clopidogrel-induced apoptosis of gastric epithelial cells. PLoS One. 2013 Sep 13;8(9):e74381. [Content Brief]

[4]. Ran Ni, et al. Effect of Different Doses of Acetylsalicylic Acid on the Antithrombotic Activity of Clopidogrel in a Mouse Arterial Thrombosis Model.Arterioscler Thromb Vasc Biol. 2018 Oct;38(10):2338-2344. [Content Brief]

[5]. Kuszynski DS, et al. Clopidogrel treatment inhibits P2Y2-Mediated constriction in the rabbit middle cerebral artery [published online ahead of print, 2021 Oct 1]. Eur J Pharmacol. 2021;174545. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.3815 mL	11.9076 mL	23.8152 mL	59.5380 mL
	5 mM	0.4763 mL	2.3815 mL	4.7630 mL	11.9076 mL
	10 mM	0.2382 mL	1.1908 mL	2.3815 mL	5.9538 mL
	15 mM	0.1588 mL	0.7938 mL	1.5877 mL	3.9692 mL
	20 mM	0.1191 mL	0.5954 mL	1.1908 mL	2.9769 mL
	25 mM	0.0953 mL	0.4763 mL	0.9526 mL	2.3815 mL
	30 mM	0.0794 mL	0.3969 mL	0.7938 mL	1.9846 mL
DMSO	40 mM	0.0595 mL	0.2977 mL	0.5954 mL	1.4884 mL
	50 mM	0.0476 mL	0.2382 mL	0.4763 mL	1.1908 mL
	60 mM	0.0397 mL	0.1985 mL	0.3969 mL	0.9923 mL
	80 mM	0.0298 mL	0.1488 mL	0.2977 mL	0.7442 mL
	100 mM	0.0238 mL	0.1191 mL	0.2382 mL	0.5954 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Clopidogrel hydrogen sulfate

32 Cited Publications

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Clopidogrel hydrogen sulfate

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Publications Citing Use of MedChemExpress (MCE) Clopidogrel hydrogen sulfate

All Cytochrome P450 Isoforms

All P2Y Receptor Isoforms

Biological Activity

Chemical Information

Publications (32)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Clopidogrel hydrogen sulfate Related Classifications

HY-17459 Related Classifications