Purification and Characterization of Novel Collagen Peptides against Platelet Aggregation and Thrombosis from Salmo salar

  • ACS Omega. 2020 Aug 4;5(32):19995-20003. doi: 10.1021/acsomega.0c01340.
Yijie Yang  1 ,  Bo Wang  1 ,  Qi Tian  1 ,  Bo Li  1  2
Affiliations
  • 1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
  • 2. Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, China.
PMID: 32832753 DOI: 10.1021/acsomega.0c01340
Abstract

Collagen is a rich source of bioactive peptides and is widely distributed in the skin and bone tissue. In this study, Collagen from Salmo salar skin was hydrolyzed with Alcalase or Protamex followed by simulated digestion, YMC ODS-A C18 separation, and ESI-MS/MS analysis. A total of 19 peptides were identified and synthesized for investigation of their antiplatelet activities. Hyp-Gly-Glu-Phe-Gly (OGEFG) and Asp-Glu-Gly-Pro (DEGP) exhibited the most potent activity against ADP-induced platelet aggregation among them with IC50 values of 277.17 and 290.00 μM, respectively, and inhibited the release of β-TG and 5-HT in a dose-dependent manner significantly. Single oral administration of OGEFG and DEGP also inhibited thrombus formation in a ferric chloride-induced arterial thrombosis model at a dose of 200 μmol/kg body weight and did not prolong the bleeding time or cause an immune response in mice. Therefore, our findings indicated that Collagen peptides had a potential to be developed into an effective specific medical food in the prevention of thrombotic diseases.

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