Estrone (Synonyms: E1; Oestrone)

Name: Estrone
Brand: MedChemExpress
SKU: HY-B0234
Rating: 5.0 (5 reviews)

Cat. No.: HY-B0234 Purity: 99.96%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Estrone (E1) is a natural estrogenic hormone. Estrone is the main representative of the endogenous estrogens and is produced by several tissues, especially adipose tissue. Estrone is the result of the process of aromatization of androstenedione that occurs in fat cells.

For research use only. We do not sell to patients.

CAS No. : 53-16-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Estrone:

Estrone O-sulfamate In-stock
Estrone-¹³C₃ In-stock
Estrone-d₂ In-stock
Estrone-d₄ In-stock
Estrone-d₂-1 In-stock
Estrone (Standard) In-stock
Estrone-¹³C₂ Get quote

5 Publications Citing Use of MCE Estrone

RT-PCR

Cell Imaging/Staining

: Estrone purchased from MedChemExpress. Usage Cited in: Physiol Rep. 2024 Sep;12(17):e70047. [Abstract]; IMR-90 and MLg cells were treated with 100 nM E1 (Estrone) or E2 for 6 h. TIMP-1 mRNA expression was measured using qPCR. The results showed that both E1 (Estrone) and E2 could induce TIMP-1 expression in IMR-90 cells at the mRNA level.

: Estrone purchased from MedChemExpress. Usage Cited in: Physiol Rep. 2024 Sep;12(17):e70047. [Abstract]; MLg cells were treated with or without 100 nM E1 (Estrone) for 24 h as indicated. MLg cells were immunostained for TIMP-1 (green). The nuclei were stained with DAPI. The results showed that TIMP-1 protein was induced in MLg fibroblasts 24 h after treatment with E1 (Estrone). Scale bar = 100 μm. ns, p > 0.05.

: Estrone purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2020 Dec;24(23):13775-13788. [Abstract]; The mRNA and protein levels of CLMP from male primary fibroblasts in the estrogen (Estrone (300 pg/mL; 48 h)) treatment and normal group.

: Estrone purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2020 Dec;24(23):13775-13788. [Abstract]; The mRNA and protein levels of CLMP from female primary fibroblasts in the estrogen ((Estrone (300 pg/mL; 48 h)) ) treatment and normal group.

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Human Endogenous Metabolite Microbial Metabolite

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	600 nM Compound: E1	Inhibition of 17beta-HSD1 expressed in HEK 293 cells assessed as conversion of [14C]estrone to [14C]estradiol Inhibition of 17beta-HSD1 expressed in HEK 293 cells assessed as conversion of [14C]estrone to [14C]estradiol	[PMID: 18372081]
HEK293	IC₅₀	810 nM Compound: E1	Inhibition of 17beta-HSD1 expressed in HEK 293 cells assessed as conversion of [14C]estrone to [14C]estradiol using NADH Inhibition of 17beta-HSD1 expressed in HEK 293 cells assessed as conversion of [14C]estrone to [14C]estradiol using NADH	[PMID: 18372081]
HeLa	EC₅₀	0.7 nM Compound: estrone	Activation of estrogen response element in HeLa cells stably transfected with human Estrogen receptor alpha. Activation of estrogen response element in HeLa cells stably transfected with human Estrogen receptor alpha.	[PMID: 11906280]
HeLa	EC₅₀	166 nM Compound: estrone	Inhibition of ER beta-mediated transactivation of ERE in HeLa cell luciferase assay at 10 uM Inhibition of ER beta-mediated transactivation of ERE in HeLa cell luciferase assay at 10 uM	[PMID: 16610787]
HeLa	EC₅₀	2.1 nM Compound: estrone	Activation of estrogen response element in HeLa cells stably transfected with human Estrogen receptor beta. Activation of estrogen response element in HeLa cells stably transfected with human Estrogen receptor beta.	[PMID: 11906280]
HeLa	EC₅₀	26 nM Compound: estrone	Inhibition of ER beta-mediated transactivation of ERE in HeLa cell luciferase assay Inhibition of ER beta-mediated transactivation of ERE in HeLa cell luciferase assay	[PMID: 16610787]
K562	IC₅₀	3 μM Compound: Estrone	TP_TRANSPORTER: drug resistance(Mitoxantrone) in BCRP-expressing K562 cells TP_TRANSPORTER: drug resistance(Mitoxantrone) in BCRP-expressing K562 cells	[PMID: 12533678]
K562	IC₅₀	> 10 μM Compound: Estrone	TP_TRANSPORTER: drug resistance(SN-38) in BCRP-expressing K562 cells TP_TRANSPORTER: drug resistance(SN-38) in BCRP-expressing K562 cells	[PMID: 12533678]
MCF7	EC₅₀	7.72 x 10^-9 M Compound: 35	Growth response in cultures of MCF-7 (human breast cancer cell line) cells Growth response in cultures of MCF-7 (human breast cancer cell line) cells	[PMID: 9357533]
Sf9	IC₅₀	96 nM Compound: 1, E1	Displacement of [3H]17beta-estradiol from human ERalpha expressed in SF9 cells Displacement of [3H]17beta-estradiol from human ERalpha expressed in SF9 cells	[PMID: 19836949]
T47D	IC₅₀	0.33 μM Compound: E1	Inhibition of 17-beta HSD1 in T47D cells Inhibition of 17-beta HSD1 in T47D cells	[PMID: 16480268]
T47D	IC₅₀	218 nM Compound: E1, estrone	Inhibition of 17beta-HSD1 in human T47D cells assessed as inhibition of transformation of [14C]-estrone into [14C]estrogen Inhibition of 17beta-HSD1 in human T47D cells assessed as inhibition of transformation of [14C]-estrone into [14C]estrogen	[PMID: 18035543]

In Vitro

Estrone is the main endogenous estrogen in postmenopausal women^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

270.37

Formula

C₁₈H₂₂O₂

CAS No.

53-16-7

Appearance

Solid

Color

White to off-white

SMILES

C[C@]1([C@](CC2)([H])[C@]3([H])CCC4=C(C=CC(O)=C4)[C@@]3([H])CC1)C2=O

Structure Classification

Steroids

Initial Source

Microorganisms

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (92.47 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6986 mL	18.4932 mL	36.9864 mL
5 mM	0.7397 mL	3.6986 mL	7.3973 mL
10 mM	0.3699 mL	1.8493 mL	3.6986 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (9.25 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (275 KB) SDS (418 KB)

COA (251 KB) HNMR (251 KB) RP-HPLC (248 KB) MS (67 KB)

Handling Instructions (2659 KB)

References

[1]. Caupos E, et al. Photodegradation of estrone enhanced by dissolved organic matter under simulated sunlight. Water Res. 2011;45(11):3341-3350. [Content Brief]

[2]. de Padua Mansur A, et al. Long-term prospective study of the influence of estrone levels on events in postmenopausal women with or at high risk for coronary artery disease. ScientificWorldJournal. 2012;2012:363595. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.6986 mL	18.4932 mL	36.9864 mL	92.4659 mL
	5 mM	0.7397 mL	3.6986 mL	7.3973 mL	18.4932 mL
	10 mM	0.3699 mL	1.8493 mL	3.6986 mL	9.2466 mL
	15 mM	0.2466 mL	1.2329 mL	2.4658 mL	6.1644 mL
	20 mM	0.1849 mL	0.9247 mL	1.8493 mL	4.6233 mL
	25 mM	0.1479 mL	0.7397 mL	1.4795 mL	3.6986 mL
	30 mM	0.1233 mL	0.6164 mL	1.2329 mL	3.0822 mL
	40 mM	0.0925 mL	0.4623 mL	0.9247 mL	2.3116 mL
	50 mM	0.0740 mL	0.3699 mL	0.7397 mL	1.8493 mL
	60 mM	0.0616 mL	0.3082 mL	0.6164 mL	1.5411 mL
	80 mM	0.0462 mL	0.2312 mL	0.4623 mL	1.1558 mL

Estrone Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Estrone53-16-7E1 OestroneE 1E-1Estrogen Receptor/ERREndogenous MetaboliteestrogenichormonepostmenopausalInhibitorinhibitorinhibit

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Estrone (Synonyms: E1; Oestrone)

Customer Review

Other Forms of Estrone:

Estrone Related Antibodies

5 Publications Citing Use of MCE Estrone

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Estrogen Receptor/ERR Isoform Specific Products:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Estrone Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Estrone Related Classifications

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