1. GPCR/G Protein
  2. Prostaglandin Receptor
  3. MF498

MF498 is a selective and orally active E prostanoid Receptor 4 (EP4) antagonist with a Ki value of 0.7 nM. MF498 can be used in the research of inflammation, such as rheumatoid and osteoarthritis.

For research use only. We do not sell to patients.

MF498 Chemical Structure

MF498 Chemical Structure

CAS No. : 915191-42-3

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 367 In-stock
Solution
10 mM * 1 mL in DMSO USD 367 In-stock
Solid
1 mg USD 62 In-stock
5 mg USD 276 In-stock
10 mg USD 504 In-stock
50 mg   Get quote  
100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE MF498

WB

    MF498 purchased from MedChemExpress. Usage Cited in: J Virol. 2018 Sep 26;92(20):e01018-18.  [Abstract]

    A549 cells are added with an EP2 or an EP4 inhibitor. After 48 h, cells are subjected to WB with an anti-Axl antibody.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    MF498 is a selective and orally active E prostanoid Receptor 4 (EP4) antagonist with a Ki value of 0.7 nM. MF498 can be used in the research of inflammation, such as rheumatoid and osteoarthritis[1][2].

    IC50 & Target[1]

    EP4

    0.7 nM (Ki)

    In Vitro

    MF498 (0.01 nM-10 μM, 30 min) inhibits PGE2-stimulated cAMP accumulation with IC50 values of 1.7 and 17 nM in the absence and presence of 10% serum, respectively[1].
    MF498 (0.01 and 0.1 μM, 30 min) inhibits FBS and PDGF-BB-induced VSMC proliferation in rat VSMCs[2].
    MF498 (0.1 μM, 30 min) reduced PDGF-induced cyclin D1 and PCNA expression in rat VSMCs[2].
    MF498 (0.1 μM, 30 min) inhibits the PDGF-BB-induced migration[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[2]

    Cell Line: Rat VSMCs
    Concentration: 0.01 nM-10 μM
    Incubation Time: 30 min before FBS treating.
    Result: Inhibited FBS and PDGF-BB-induced VSMC proliferation.
    In Vivo

    MF498 (0-20 mg/kg, p.o.) inhibits paw swelling in rats with adjuvant-induced arthritis (AIA)[1].
    MF498 (0-30 mg/kg, p.o.) attenuates the EP4 agonist-(L-902688)-induced hyperalgesia in guinea pigs[1].
    MF498 (20 mg/kg, p.o.) shows 100% bioavailability, resulting in plasma concentration of 1.2 μM at 6 h[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Rats with adjuvant-induced arthritis (AIA)[1]
    Dosage: 0, 0.008, 0.04, 0.2, 2, 20 mg/kg
    Administration: Oral administration (p.o.)
    Result: Showed anti-inflammatory effect in both the primary and secondary paw.
    Molecular Weight

    603.69

    Formula

    C32H33N3O7S

    CAS No.
    Appearance

    Solid

    Color

    Pink to yellow

    SMILES

    O=C1N(C2=C(C)C=C(CS(NC(CC3=CC=CC=C3OC)=O)(=O)=O)C=C2)CC4=C(OCC)C5=NC=CC=C5C(OCC)=C41

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 31 mg/mL (51.35 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6565 mL 8.2824 mL 16.5648 mL
    5 mM 0.3313 mL 1.6565 mL 3.3130 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.14 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (4.14 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.26%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.6565 mL 8.2824 mL 16.5648 mL 41.4120 mL
    5 mM 0.3313 mL 1.6565 mL 3.3130 mL 8.2824 mL
    10 mM 0.1656 mL 0.8282 mL 1.6565 mL 4.1412 mL
    15 mM 0.1104 mL 0.5522 mL 1.1043 mL 2.7608 mL
    20 mM 0.0828 mL 0.4141 mL 0.8282 mL 2.0706 mL
    25 mM 0.0663 mL 0.3313 mL 0.6626 mL 1.6565 mL
    30 mM 0.0552 mL 0.2761 mL 0.5522 mL 1.3804 mL
    40 mM 0.0414 mL 0.2071 mL 0.4141 mL 1.0353 mL
    50 mM 0.0331 mL 0.1656 mL 0.3313 mL 0.8282 mL
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    MF498 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    MF498
    Cat. No.:
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