1. GPCR/G Protein
  2. Adenosine Receptor
  3. 5'-N-Ethylcarboxamidoadenosine

5'-N-Ethylcarboxamidoadenosine (Synonyms: NECA)

Cat. No.: HY-103173 Purity: 99.86%
Handling Instructions

5'-N-Ethylcarboxamidoadenosine (NECA) is a nonselective adenosine receptor agonist.

For research use only. We do not sell to patients.

5'-N-Ethylcarboxamidoadenosine Chemical Structure

5'-N-Ethylcarboxamidoadenosine Chemical Structure

CAS No. : 35920-39-9

Size Price Stock Quantity
10 mM * 1  mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 78 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Publications Citing Use of MCE 5'-N-Ethylcarboxamidoadenosine

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Description

5'-N-Ethylcarboxamidoadenosine (NECA) is a nonselective adenosine receptor agonist.

IC50 & Target

Adenosine receptor[1]

In Vivo

After the administration of 5'-N-Ethylcarboxamidoadenosine (NECA), the mean number of cocaine infusions obtained per session is decreased significantly in a dose-dependent manner [5'-N-Ethylcarboxamidoadenosine (NECA): F(4,12)=14.9; P<0.001]. The administration of 5'-N-Ethylcarboxamidoadenosine (NECA) [F(4,12)=16.1; P<0.001] results in a significant increase in latencies above values obtained for vehicle treatment[1]. Daily i.p. injection of 5'-N-Ethylcarboxamidoadenosine (NECA) at 0.3 mg/kg/day for two weeks reduces malondialdehyde (MDA) levels in diabetic rats, but does not affect control rats. Daily treatment with NECA (0.3 mg/kg/day, i.p. for two weeks) reduces diabetes-induced gene expression of tumor necrosis factor (TNF)-α and interleukin (IL)-18 in diabetic rats, but does not affect control rats. Daily i.p. injection of 5'-N-Ethylcarboxamidoadenosine (NECA) at 0.3 mg/kg/day for two weeks also blocks the activation of JNK MAPK in diabetic rats, but does not affect control rats[2].

Molecular Weight

308.29

Formula

C₁₂H₁₆N₆O₄

CAS No.

35920-39-9

SMILES
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 150 mg/mL (486.55 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2437 mL 16.2185 mL 32.4370 mL
5 mM 0.6487 mL 3.2437 mL 6.4874 mL
10 mM 0.3244 mL 1.6218 mL 3.2437 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (8.11 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (8.11 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.11 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Animal Administration
[1]

Male Wistar rats are used in this experiment. These animals range in weight between 350 and 425 g. They are housed individually in hanging wire cages under a 12-h light/dark cycle. The effects of 5'-N-Ethylcarboxamidoadenosine (NECA) are tested in four rats. In the experiment, either 5'-N-Ethylcarboxamidoadenosine (NECA) (5, 7.5, 10, 20 μg/kg) or vehicle (saline) is administered intraperitoneally 15 min prior to the start of test sessions. 5'-N-Ethylcarboxamidoadenosine (NECA) is administered following a random order crossover design. In most cases, animals are tested twice with the same dose[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.86%

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Keywords:

5'-N-EthylcarboxamidoadenosineNECAAdenosine ReceptorP1 receptorInhibitorinhibitorinhibit

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5'-N-Ethylcarboxamidoadenosine
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