NVP-2
Based on 41 publication(s) in Google Scholar
NVP-2 is a potent and selective ATP-competitive cyclin dependent kinase 9 (CDK9) probe, inhibits CDK9/CycT activity with an IC50 of 0.514 nM. NVP-2 displays inhibitory effcts on CDK1/CycB, CDK2/CycA and CDK16/CycY kinases with IC50 values of 0.584 μM, 0.706 μM, and 0.605 μM, respectively. NVP-2 induces cell apoptosis.
For research use only. We do not sell to patients.
- Purity: 99.05%
- CAS No.: 1263373-43-8
- Formula: C27H37ClN6O2
- Molecular Weight:513.07
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
Publications Citing Use of MedChemExpress (MCE) NVP-2
More- Nature. 2026 Jan;649(8098):1032-1041. [Abstract]
- Nature. 2024 Apr;628(8007):408-415. [Abstract]
- Cancer Cell. 2019 May 13;35(5):752-766.e9. [Abstract]
- Cell. 2018 Sep 20;175(1):171-185.e25. [Abstract]
- Mol Cell. 2025 May 15;85(10):1952-1967.e8. [Abstract]
- Mol Cell. 2025 Apr 3;85(7):1280-1295.e9. [Abstract]
- Cancer Res. 2024 Jan 2;84(1):17-25. [Abstract]
- Mol Cell. 2021 Nov 4;81(21):4413-4424.e5. [Abstract]
- Mol Cell. 2020 Apr 16;78(2):261-274.e5. [Abstract]
- Cancer Res. 2019 Jul 1;79(13):3479-3491. [Abstract]
- Nat Commun. 2026 Feb 12;17(1):1214. [Abstract]
- Nat Commun. 2024 Aug 17;15(1):7089. [Abstract]
- Nat Commun. 2021 Nov 16;12(1):6607. [Abstract]
- Nat Commun. 2019 Oct 18;10(1):4741. [Abstract]
- Nucleic Acids Res. 2023 Apr 11;51(6):e32. [Abstract]
- Sci Adv. 2025 Jan 24;11(4):eadt5885. [Abstract]
- J Biomed Sci. 2022 Feb 14;29(1):13. [Abstract]
- Proc Natl Acad Sci U S A. 2022 Feb 1;119(5):e2120687119. [Abstract]
- Neoplasia. 2019 Jul;21(7):713-720. [Abstract]
- Cell Chem Biol. 2021 Feb 18;28(2):134-147.e14. [Abstract]
- Cell Rep. 2024 Oct 8;43(10):114792. [Abstract]
- Cell Rep. 2021 Mar 16;34(11):108870. [Abstract]
- J Med Chem. 2024 Sep 12;67(17):15220-15245. [Abstract]
- J Med Chem. 2021 Oct 14;64(19):14647-14663. [Abstract]
- Sci Signal. 2019 Jul 16;12(590). pii: eaav7259. [Abstract]
- Mol Cancer Res. 2020 Oct;18(10):1512-1521. [Abstract]
- Cancer Biol Ther. 2025 Dec;26(1):2450859. [Abstract]
- Mol Oncol. 2023 Dec;17(12):2507-2525. [Abstract]
- J Cell Mol Med. 2026 Apr;30(7):e71101. [Abstract]
- iScience. 2025 Nov 4;28(12):113925. [Abstract]
- RNA Biol. 2021 Nov 12;18(sup2):722-729. [Abstract]
- J Cancer Res Clin Oncol. 2023 Jul;149(8):5255-5263. [Abstract]
- Biochem Biophys Res Commun. 2023 Sep 3:671:75-79. [Abstract]
- bioRxiv. 2025 Oct 8:2025.10.08.681243. [Abstract]
- bioRxiv. 2025 March 19.
- Patent. US20240425555A1.
- University of California, Irvine. 2024.
- bioRxiv. 2024 Jul 26:2024.07.25.605008. [Abstract]
- bioRxiv. 2024 July 08.
- bioRxiv. 2023 Mar 23.
- Universidade de Lisboa. 2021 Dec 21.
-
WB
-
WB
-
WB
Biological Activity
|
CDK9 0.5 nM (IC50) |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
5.78 μM
Compound: NVP-2; 5
|
Antiproliferative activity against human A549 cells incubated for 72 hrs by celltiter-glo luminescent assay
Antiproliferative activity against human A549 cells incubated for 72 hrs by celltiter-glo luminescent assay
|
[PMID: 39178382] |
| CESS | IC50 |
0.01 μM
Compound: NVP-2; 5
|
Antiproliferative activity against human CESS cells incubated for 72 hrs by celltiter-glo luminescent assay
Antiproliferative activity against human CESS cells incubated for 72 hrs by celltiter-glo luminescent assay
|
[PMID: 39178382] |
| HT-29 | IC50 |
0.02 μM
Compound: NVP-2; 5
|
Antiproliferative activity against human HT-29 cells incubated for 72 hrs by celltiter-glo luminescent assay
Antiproliferative activity against human HT-29 cells incubated for 72 hrs by celltiter-glo luminescent assay
|
[PMID: 39178382] |
| Jurkat | GI50 |
<0.16 μM
Compound: NVP-2
|
Antiproliferative activity against human Jurkat cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK8 assay
Antiproliferative activity against human Jurkat cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK8 assay
|
[PMID: 32129996] |
| KG-1 | IC50 |
0.02 μM
Compound: NVP-2; 5
|
Antiproliferative activity against human KG-1 cells incubated for 72 hrs by celltiter-glo luminescent assay
Antiproliferative activity against human KG-1 cells incubated for 72 hrs by celltiter-glo luminescent assay
|
[PMID: 39178382] |
| L02 | GI50 |
28.33 μM
Compound: NVP-2
|
Cytotoxicity against human L02 cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK8 assay
Cytotoxicity against human L02 cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK8 assay
|
[PMID: 32129996] |
| MCF7 | GI50 |
43.36 μM
Compound: NVP-2
|
Antiproliferative activity against human MCF7 cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK8 assay
Antiproliferative activity against human MCF7 cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK8 assay
|
[PMID: 32129996] |
| MDA-MB-231 | GI50 |
<0.16 μM
Compound: NVP-2
|
Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK8 assay
Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK8 assay
|
[PMID: 32129996] |
| MV4-11 | IC50 |
0.003 μM
Compound: NVP-2; 5
|
Antiproliferative activity against human MV4-11 cells incubated for 72 hrs by celltiter-glo luminescent assay
Antiproliferative activity against human MV4-11 cells incubated for 72 hrs by celltiter-glo luminescent assay
|
[PMID: 39178382] |
| PC-9 | IC50 |
0.03 μM
Compound: NVP-2; 5
|
Antiproliferative activity against human PC-9 cells incubated for 72 hrs by celltiter-glo luminescent assay
Antiproliferative activity against human PC-9 cells incubated for 72 hrs by celltiter-glo luminescent assay
|
[PMID: 39178382] |
| Rec1 | IC50 |
0.03 μM
Compound: NVP-2; 5
|
Antiproliferative activity against human REC1 cells incubated for 72 hrs by celltiter-glo luminescent assay
Antiproliferative activity against human REC1 cells incubated for 72 hrs by celltiter-glo luminescent assay
|
[PMID: 39178382] |
| Sf9 | IC50 |
>10 μM
Compound: NVP-2
|
Inhibition of recombinant human full-length N-terminal His-tagged CDK6/cyclinD3 expressed in baculovirus infected Sf9 insect cells using histone H1 as substrate measured after 60 mins by ADP-glo assay
Inhibition of recombinant human full-length N-terminal His-tagged CDK6/cyclinD3 expressed in baculovirus infected Sf9 insect cells using histone H1 as substrate measured after 60 mins by ADP-glo assay
|
[PMID: 32129996] |
| Sf9 | IC50 |
0.002 μM
Compound: NVP-2
|
Inhibition of recombinant human full-length N-terminal GST-tagged CDK9/cyclinK expressed in baculovirus infected Sf9 insect cells using PDKtide as substrate measured after 120 mins by ADP-glo assay
Inhibition of recombinant human full-length N-terminal GST-tagged CDK9/cyclinK expressed in baculovirus infected Sf9 insect cells using PDKtide as substrate measured after 120 mins by ADP-glo assay
|
[PMID: 32129996] |
| Sf9 | IC50 |
3.46 μM
Compound: NVP-2
|
Inhibition of recombinant human full-length N-terminal GST-tagged CDK2/cyclinA2 expressed in baculovirus infected Sf9 insect cells using histone H1 as substrate measured after 10 mins by ADP-glo assay
Inhibition of recombinant human full-length N-terminal GST-tagged CDK2/cyclinA2 expressed in baculovirus infected Sf9 insect cells using histone H1 as substrate measured after 10 mins by ADP-glo assay
|
[PMID: 32129996] |
NVP-2 has an anti-proliferation of leukemia cells, inhibits KOPT-K1, Jurkat, P12-ICHIKAWA, DU.528, MOLT 16, HSB-2, PF-382, SKW-3, SUP-T11, DND-41 and HPB-ALL cells with IC50 values of 0.1688 μM, 0.1233 μM,0.5736 μM,0.1575 μM, 0.1620 μM,0.1585 μM, 0.1808 μM, 0.2589 μM, 0.0918 μM and 0.3023 μM, respectively[1]. NVP-2 (250 nM-1 μM; 6 hours) engages CDK9 in wildtype and CRBN / MOLT4 cells at all concentrations, while CDK2 and CDK7 are unaffected[1]. NVP-2 (0-10 nM; 72 hours) exhibits CRBN-dependent anti-proliferative and pro-apoptotic effects in MOLT4 cells, displays an IC50 value of 9 nM[1]. NVP-2 (250 nM; 24 hours) induces cell apoptosis in MOLT4 cells, upregulates caspase-3 and γH2A.X expression. However, while the compound washout significantly reduces the degree of apoptosis induced by NVP-2[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Leukemia cell lines
-
Concentration:0.1233 µM-0.5736 µM
-
Incubation Time:72 hours
-
Result:Inhibited Leukemia cell lines viabity.
-
Cell Line:Wildtype and CRBN−/− MOLT4 cells
-
Concentration:1 μM; 500 nM; 250 nM
-
Incubation Time:6 hours
-
Result:Degrade CDK9 sub-stoichiometrically at all concentrations.
-
Cell Line:Wildtype and CRBN−/− MOLT4 cells
-
Concentration:250 nM
-
Incubation Time:24 hours
-
Result:Induced cell apoptosis in cells and wash outed the compound relieved NVP-2-induced cell apoptosis.
-
Cell Line:MOLT4 cells
-
Concentration:0-10 nM
-
Incubation Time:72 hours
-
Result:Exhibited anti-proliferative effects in MOLT4 cells.
Chemical Information
-
CAS No. 1263373-43-8
-
Appearance Solid
-
Molecular Weight 513.07
-
Formula C27H37ClN6O2
-
Color White to yellow
-
SMILES
ClC1=CN=C(N[C@H]2CC[C@H](N[C@H](C)COC)CC2)C=C1C3=CC=CC(NCC4(C#N)CCOCC4)=N3
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (41)
-
Journal Impact Factor
-
Most Recent
-
Nature
2026 Jan;649(8098):1032-1041. PMID: 41299171 -
Nature
2024 Apr;628(8007):408-415. PMID: 38480883 -
Cancer Cell
BCL2 Amplicon Loss and Transcriptional Remodeling Drives ABT-199 Resistance in B Cell Lymphoma Models. [Abstract]2019 May 13;35(5):752-766.e9. PMID: 31085176 -
Cell
Small Molecules Co-targeting CKIα and the Transcriptional Kinases CDK7/9 Control AML in Preclinical Models. [Abstract]2018 Sep 20;175(1):171-185.e25. PMID: 30146162
NVP-2 purchased from MedChemExpress. Usage Cited in: Cell. 2018 Sep 20;175(1):171-185.e25. [Abstract]
WB analysis of MV4-11 cells treated with BTX161 (6 hr), iCDK9 (4 hr), or THZ1 (4 hr) at the indicated concentrations in different combinations as indicated. PP2Ac is a loading control.
-
Mol Cell
PAF1C-mediated activation of CDK12/13 kinase activity is critical for CTD phosphorylation and transcript elongation. [Abstract]2025 May 15;85(10):1952-1967.e8. PMID: 40315851 -
Mol Cell
Transcription-coupled AID deamination damage depends on ELOF1-associated RNA polymerase II. [Abstract]2025 Apr 3;85(7):1280-1295.e9. PMID: 40049162 -
Cancer Res
Targeting transcriptional regulation with a CDK9 inhibitor suppresses growth of endocrine- and palbociclib-resistant ER+ breast cancers. [Abstract]2024 Jan 2;84(1):17-25. PMID: 37801608 -
Mol Cell
2021 Nov 4;81(21):4413-4424.e5. PMID: 34480849 -
Mol Cell
2020 Apr 16;78(2):261-274.e5. PMID: 32155413 -
Cancer Res
2019 Jul 1;79(13):3479-3491. PMID: 31064851 -
Nat Commun
Human iPSC-based Modeling of Pulmonary Fibrosis Reveals p300/CBP Inhibition Suppresses Alveolar Transitional Cell State. [Abstract]2026 Feb 12;17(1):1214. PMID: 41680175 -
Nat Commun
Coordination of transcription-coupled repair and repair-independent release of lesion-stalled RNA polymerase II. [Abstract]2024 Aug 17;15(1):7089. PMID: 39154022 -
Nat Commun
Abemaciclib is a potent inhibitor of DYRK1A and HIP kinases involved in transcriptional regulation. [Abstract]2021 Nov 16;12(1):6607. PMID: 34785661 -
Nat Commun
2019 Oct 18;10(1):4741. PMID: 31628323 -
Nucleic Acids Res
2023 Apr 11;51(6):e32. PMID: 36715337 -
Sci Adv
2025 Jan 24;11(4):eadt5885. PMID: 39854452 -
J Biomed Sci
O-GlcNAc transferase couples MRE11 to transcriptionally active chromatin to suppress DNA damage. [Abstract]2022 Feb 14;29(1):13. PMID: 35164752 -
Proc Natl Acad Sci U S A
Function-guided proximity mapping unveils electrophilic-metabolite sensing by proteins not present in their canonical locales. [Abstract]2022 Feb 1;119(5):e2120687119. PMID: 35082156 -
Neoplasia
CDK9 Inhibition Induces a Metabolic Switch that Renders Prostate Cancer Cells Dependent on Fatty Acid Oxidation. [Abstract]2019 Jul;21(7):713-720. PMID: 31151054
NVP-2 purchased from MedChemExpress. Usage Cited in: Neoplasia. 2019 Jul;21(7):713-720. [Abstract]
LNCaP cells were treated with DMSO or increasing dose of NVP2 for 24 hours and samples are analyzed using western blotting. Densitometry is used to evaluate the abundance of each protein.
-
Cell Chem Biol
Modulating Androgen Receptor-Driven Transcription in Prostate Cancer with Selective CDK9 Inhibitors. [Abstract]2021 Feb 18;28(2):134-147.e14. PMID: 33086052 -
Cell Rep
ICP22-defined condensates mediate RNAPII deubiquitylation by UL36 and promote HSV-1 transcription. [Abstract]2024 Oct 8;43(10):114792. PMID: 39383039 -
Cell Rep
Transcriptional programming drives Ibrutinib-resistance evolution in mantle cell lymphoma. [Abstract]2021 Mar 16;34(11):108870. PMID: 33730585
NVP-2 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2021 Mar 16;34(11):108870. [Abstract]
NVP-2 treatment suppresses RNA polymerase II (RNAPII) CTD pSer2, pAKT, MYC, and MCL-1 levels and augments PARP cleavage in primary MCL patient samples (Pt0448, Pt1888) in a dose-dependent fashion.
-
J Med Chem
Discovery of Novel 2-Aminopyridine-Based and 2-Aminopyrimidine-Based Derivatives as Potent CDK/HDAC Dual Inhibitors for the Treatment of Refractory Solid Tumors and Hematological Malignancies. [Abstract]2024 Sep 12;67(17):15220-15245. PMID: 39178382 -
J Med Chem
Rational Design and Development of Novel CDK9 Inhibitors for the Treatment of Acute Myeloid Leukemia. [Abstract]2021 Oct 14;64(19):14647-14663. PMID: 34477384 -
Sci Signal
Application of a MYC degradation screen identifies sensitivity to CDK9 inhibitors in KRAS-mutant pancreatic cancer. [Abstract]2019 Jul 16;12(590). pii: eaav7259. PMID: 31311847 -
Mol Cancer Res
2020 Oct;18(10):1512-1521. PMID: 32611550 -
Cancer Biol Ther
NVP-2, in combination with Orlistat, represents a promising therapeutic strategy for acute myeloid leukemia. [Abstract]2025 Dec;26(1):2450859. PMID: 39800696 -
Mol Oncol
Dinaciclib synergizes with BH3 mimetics targeting BCL-2 and BCL-XL in multiple myeloma cell lines partially-dependent on MCL-1 and in plasma cells from patients. [Abstract]2023 Dec;17(12):2507-2525. PMID: 37704591 -
J Cell Mol Med
2026 Apr;30(7):e71101. PMID: 41896195 -
iScience
2025 Nov 4;28(12):113925. PMID: 41321622 -
RNA Biol
2021 Nov 12;18(sup2):722-729. PMID: 34592899 -
J Cancer Res Clin Oncol
2023 Jul;149(8):5255-5263. PMID: 36401094 -
Biochem Biophys Res Commun
Brd4-dependent CDK9 expression induction upon sustained pharmacological inhibition of P-TEFb kinase activity. [Abstract]2023 Sep 3:671:75-79. PMID: 37295357 -
bioRxiv
2025 Oct 8:2025.10.08.681243. PMID: 41279442 -
-
-
-
bioRxiv
Resistance of estrogen receptor function to BET bromodomain inhibition is mediated by transcriptional coactivator cooperativity. [Abstract]2024 Jul 26:2024.07.25.605008. PMID: 39211208 -
-
-
Solvent & Solubility
DMSO : ≥ 100 mg/mL (194.91 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.87 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.87 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (277 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9491 mL | 9.7453 mL | 19.4905 mL | 48.7263 mL |
| 5 mM | 0.3898 mL | 1.9491 mL | 3.8981 mL | 9.7453 mL | |
| 10 mM | 0.1949 mL | 0.9745 mL | 1.9491 mL | 4.8726 mL | |
| 15 mM | 0.1299 mL | 0.6497 mL | 1.2994 mL | 3.2484 mL | |
| 20 mM | 0.0975 mL | 0.4873 mL | 0.9745 mL | 2.4363 mL | |
| 25 mM | 0.0780 mL | 0.3898 mL | 0.7796 mL | 1.9491 mL | |
| 30 mM | 0.0650 mL | 0.3248 mL | 0.6497 mL | 1.6242 mL | |
| 40 mM | 0.0487 mL | 0.2436 mL | 0.4873 mL | 1.2182 mL | |
| 50 mM | 0.0390 mL | 0.1949 mL | 0.3898 mL | 0.9745 mL | |
| 60 mM | 0.0325 mL | 0.1624 mL | 0.3248 mL | 0.8121 mL | |
| 80 mM | 0.0244 mL | 0.1218 mL | 0.2436 mL | 0.6091 mL | |
| 100 mM | 0.0195 mL | 0.0975 mL | 0.1949 mL | 0.4873 mL |