Taxifolin (Synonyms: (+)-Dihydroquercetin; (+)-Taxifolin)

Name: Taxifolin
Brand: MedChemExpress
SKU: HY-N0136
Rating: 5.0 (27 reviews)

Cat. No.: HY-N0136 Purity: 99.92%: Data Sheet
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Taxifolin ((+)-Dihydroquercetin) exhibits important anti-tyrosinase activity. Taxifolin exhibits significant inhibitory activity against collagenase with an IC₅₀ value of 193.3 μM. Taxifolin is an important natural compound with antifibrotic activity. Taxifolin is a free radical scavenger with antioxidant capacity.

For research use only. We do not sell to patients.

CAS No. : 480-18-2

Size	Stock	Quantity

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 27 publication(s) in Google Scholar

Other Forms of Taxifolin:

27 Publications Citing Use of MCE Taxifolin

Bio/Physico-chemical Assay

Cell Proliferation/Viability Assay

Cell Imaging/Staining

IHC

: Taxifolin purchased from MedChemExpress. Usage Cited in: Front Immunol. 2025 Mar 17:16:1532336. [Abstract]; After infection of MDCK cells with H1N1-UI182 at an MOI of 0.01, the cells were treated with Quercetin (10 µM), Taxifolin (50 µM), Miquelianin (100 µM), and Baloxavir (10 µM) for 48 h. Nuclei were stained with DAPI, and infected cells were detected by NP-specific immunofluorescence staining (scale bar: 100 µm).

: Taxifolin purchased from MedChemExpress. Usage Cited in: Vet Sci. 2024 Mar 30;11(4):156. [Abstract]; IPEC-J2 cells were treated with Taxifolin (0, 50, 100, 150, 300, and 400 μM) for 24 h. Cell viability was determined using the CCK-8 assay.

: Taxifolin purchased from MedChemExpress. Usage Cited in: Vet Sci. 2024 Mar 30;11(4):156. [Abstract]; After pretreating the cells with 150 μM Taxifolin (0-150 μM) for 24 h, they were exposed to 0.5 μg/mL DON for another 24 h. Then, cell morphology was evaluated by capturing images of the cells. The cyan arrows indicate shrunken or fragmentated cells; the yellow arrows indicate normal or dividing cells. The magnification is 40×.

: Taxifolin purchased from MedChemExpress. Usage Cited in: Vet Sci. 2024 Mar 30;11(4):156. [Abstract]; Representative immunofluorescence images of the control, Taxifolin, DON, and Taxifolin (0-150 μM) + DON groups labeled with DAPI (blue) and anti-Ki67 antibody (red).

: Taxifolin purchased from MedChemExpress. Usage Cited in: Vet Sci. 2024 Mar 30;11(4):156. [Abstract]; Effect of Taxifolin on intracellular ROS levels. Fluorescence values were determined using a fluorescence microplate. Data are shown as mean ± SEM (n = 3). Control: control group; 150 μM TA: 150 μM Taxifolin group; DON: 0.5 μg/mL DON group; 150 μM Taxifolin + DON: 150 μM Taxifolin + 0.5 μg/mL DON group.

: Taxifolin purchased from MedChemExpress. Usage Cited in: Molecules. 2021 Mar 5;26(5):1409.; Time course of SARS-CoV-2 M^pro enzymatic activity in response to inhibitory compounds. Black, green, red, and yellow dots represent control, 100 µM Taxifolin, 95.6 µM Silybin A + Silybin B, and 100 µM GC376, respectively. Reported traces are the average of three experiments.

: Taxifolin purchased from MedChemExpress. Usage Cited in: Appl Microbiol Biotechnol. 2018 Feb;102(3):1443-1453. [Abstract]; Dihydroquercetin relieves APAP-induced necrosis and suppressed ERK/JNK stress responses. Western Blot analysis for phosphor-JNK1/2, β-Actin as a lading control.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 193.3 μM (Collagenase)^[1]
Tyrosinase^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
BJ	EC₅₀	> 10 μM Compound: 1	Cytotoxicity against human BJ cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human BJ cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
CCRF-CEM	EC₅₀	> 10 μM Compound: 1	Cytotoxicity against human CEM cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human CEM cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
H9	EC₅₀	9.8 μg/mL Compound: 3	Inhibition of HIV-1 replication in H9 (human lymphoma) cells. Inhibition of HIV-1 replication in H9 (human lymphoma) cells.	10.1016/0960-894X(96)00095-9
H9	IC₅₀	9.8 μg/mL Compound: 3	Inhibition of uninfected H9 lymphocytic cell growth Inhibition of uninfected H9 lymphocytic cell growth	10.1016/0960-894X(96)00095-9
HEK293	EC₅₀	670.66 μM Compound: DHQ	Cytotoxicity in HEK293 cells assessed as reduction in cell survival incubated for 48 hrs by MTT assay Cytotoxicity in HEK293 cells assessed as reduction in cell survival incubated for 48 hrs by MTT assay	[PMID: 30108751]
HL-60	IC₅₀	10 ng/mL Compound: Taxifolin	Growth inhibition of human HL60 cells after 72 hrs by MTT assay Growth inhibition of human HL60 cells after 72 hrs by MTT assay	[PMID: 31398616]
HT-1080	IC₅₀	> 10 μM Compound: Taxifolin	Anti-ferroptotic activity against Erastin-induced human HT-1080 cells assessed as inhibition of ferroptosis incubated for 72 hrs by CCK-8 assay Anti-ferroptotic activity against Erastin-induced human HT-1080 cells assessed as inhibition of ferroptosis incubated for 72 hrs by CCK-8 assay	[PMID: 38996382]
HUVEC	IC₅₀	12 μM Compound: 43, taxifolin	Inhibition of NADPH oxidase in Homo sapiens (human) HUVEC cells Inhibition of NADPH oxidase in Homo sapiens (human) HUVEC cells	10.1007/s00044-012-0353-y
HeLa	EC₅₀	125.31 μM Compound: DHQ	Cytotoxicity in human HeLa cells assessed as reduction in cell survival incubated for 48 hrs by MTT assay Cytotoxicity in human HeLa cells assessed as reduction in cell survival incubated for 48 hrs by MTT assay	[PMID: 30108751]
HeLa	EC₅₀	> 10 μM Compound: 1	Cytotoxicity against human HeLa cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human HeLa cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
L929	EC₅₀	200 μM Compound: taxifolin	Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by crystal violet staining Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by crystal violet staining	[PMID: 9287415]
L929	EC₅₀	240 μM Compound: taxifolin	Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay	[PMID: 9287415]
MCF7	EC₅₀	> 10 μM Compound: 1	Cytotoxicity against human MCF7 cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human MCF7 cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
MT4	CC₅₀	25.4 μg/mL Compound: 14	Cytotoxicity against human MT4 cells by MTT assay Cytotoxicity against human MT4 cells by MTT assay	[PMID: 10425115]
MT4	IC₅₀	> 25.4 μg/mL Compound: 14	Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay	[PMID: 10425115]
Neutrophil	IC₅₀	15.3 μM Compound: 5c	Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of HOCl-induced oxidation of APF after 6 mins by fluorescence assay Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of HOCl-induced oxidation of APF after 6 mins by fluorescence assay	[PMID: 23871908]
Neutrophil	IC₅₀	15.5 μM Compound: 5c	Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of superoxide anion radical-induced lucigenin oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of superoxide anion radical-induced lucigenin oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay	[PMID: 23871908]
Neutrophil	IC₅₀	41.3 μM Compound: 5c	Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of H2O2-induced oxidation of amplex red incubated for 5 mins prior to PMA challenge by fluorescence assay Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of H2O2-induced oxidation of amplex red incubated for 5 mins prior to PMA challenge by fluorescence assay	[PMID: 23871908]
Neutrophil	IC₅₀	53.5 μM Compound: 5c	Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of ROS-induced luminol oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of ROS-induced luminol oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay	[PMID: 23871908]
RAW264.7	IC₅₀	81.6 μM Compound: 10	Inhibition of LPS-induced NO production in mouse RAW264.7 cells by Griess reagent based assay Inhibition of LPS-induced NO production in mouse RAW264.7 cells by Griess reagent based assay	[PMID: 32118424]
RPMI-8226	EC₅₀	> 10 μM Compound: 1	Cytotoxicity against human RPMI8226 cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human RPMI8226 cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
Sf9	IC₅₀	15.7 μM Compound: 4	Inhibition of N-terminal GST-His-tagged c-KIT (544 to 976 amino acids) D816V mutant (unknown origin) expressed in Sf9 insect cells using poly[Glu:Tyr] (4:1) as substrate preincubated for 20 mins followed by [33P-gamma]ATP addition and subsequent inhibition for 2 hrs by radiometric assay Inhibition of N-terminal GST-His-tagged c-KIT (544 to 976 amino acids) D816V mutant (unknown origin) expressed in Sf9 insect cells using poly[Glu:Tyr] (4:1) as substrate preincubated for 20 mins followed by [33P-gamma]ATP addition and subsequent inhibition for 2 hrs by radiometric assay	[PMID: 26807861]
THP-1	EC₅₀	> 10 μM Compound: 1	Cytotoxicity against human THP1 cells after 72 hrs by erythosin B staining method Cytotoxicity against human THP1 cells after 72 hrs by erythosin B staining method	[PMID: 20192247]
U-266	EC₅₀	> 10 μM Compound: 1	Cytotoxicity against human U266 cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human U266 cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]

In Vitro

This is confirmed by the investigation of pure Taxifolin and (+)-Catechin against collagenase activity. Taxifolin exhibits significant inhibitory activity with an IC₅₀ value of 193.3 μM while (+)-Catechin is not active^[1].
Taxifolin is a ubiquitous bioactive constituent of foods and herbs. Taxifolin (dihydroquercetin) is a bioactive flavanonol commonly found in grapes, citrus fruits, onions, green tea, olive oil, wine, and many other foods, as well as several herbs (such as milk thistle, French maritime bark, Douglas fir bark, and Smilacis Glabrae Rhizoma)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

304.25

Formula

C₁₅H₁₂O₇

CAS No.

480-18-2

Appearance

Solid

Color

Off-white to yellow

SMILES

O=C1[C@H](O)[C@@H](C2=CC=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C13

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (328.68 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2868 mL	16.4339 mL	32.8677 mL
5 mM	0.6574 mL	3.2868 mL	6.5735 mL
10 mM	0.3287 mL	1.6434 mL	3.2868 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL (9.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.75 mg/mL (9.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.75 mg/mL (9.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (249 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. Angelis A, et al. Bio-Guided Isolation of Methanol-Soluble Metabolites of Common Spruce (Picea abies) Bark by-Products and Investigation of Their Dermo-Cosmetic Properties. Molecules. 2016 Nov 21;21(11):1586. [Content Brief]

[2]. Lei Ren, et al. Dissecting Efficacy and Metabolic Characteristic Mechanism of Taxifolin on Renal Fibrosis by Multivariate Approach and Ultra-Performance Liquid Chromatography Coupled With Mass Spectrometry-Based Metabolomics Strategy. Front Pharmacol. 2021 Jan 14;11:608511. [Content Brief]

[3]. Yang P, et al. Detection of 191 Taxifolin Metabolites and Their Distribution in Rats Using HPLC-ESI-IT-TOF-MS(n). Molecules. 2016 Sep 13;21(9). pii: E1209. [Content Brief]

Animal Administration
^[3]

Rats^[3]
Twelve male Sprague-Dawley rats (weighing 180-220 g) are used. The rats are randomly divided into two groups (six rats per group), a drug group and a blank group. Taxifolin is suspended in 0.5% CMC-Na solution and orally administered to the drug group at a dose of 200 mg/kg body weight, while blank group rats are orally administered 0.5% CMC-Na solution at the same volume. All rats are dosed once a day (at 9:00 a.m.) for 3 days.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Angelis A, et al. Bio-Guided Isolation of Methanol-Soluble Metabolites of Common Spruce (Picea abies) Bark by-Products and Investigation of Their Dermo-Cosmetic Properties. Molecules. 2016 Nov 21;21(11):1586. [Content Brief]

[2]. Lei Ren, et al. Dissecting Efficacy and Metabolic Characteristic Mechanism of Taxifolin on Renal Fibrosis by Multivariate Approach and Ultra-Performance Liquid Chromatography Coupled With Mass Spectrometry-Based Metabolomics Strategy. Front Pharmacol. 2021 Jan 14;11:608511. [Content Brief]

[3]. Yang P, et al. Detection of 191 Taxifolin Metabolites and Their Distribution in Rats Using HPLC-ESI-IT-TOF-MS(n). Molecules. 2016 Sep 13;21(9). pii: E1209. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2868 mL	16.4339 mL	32.8677 mL	82.1693 mL
	5 mM	0.6574 mL	3.2868 mL	6.5735 mL	16.4339 mL
	10 mM	0.3287 mL	1.6434 mL	3.2868 mL	8.2169 mL
	15 mM	0.2191 mL	1.0956 mL	2.1912 mL	5.4780 mL
	20 mM	0.1643 mL	0.8217 mL	1.6434 mL	4.1085 mL
	25 mM	0.1315 mL	0.6574 mL	1.3147 mL	3.2868 mL
	30 mM	0.1096 mL	0.5478 mL	1.0956 mL	2.7390 mL
	40 mM	0.0822 mL	0.4108 mL	0.8217 mL	2.0542 mL
	50 mM	0.0657 mL	0.3287 mL	0.6574 mL	1.6434 mL
	60 mM	0.0548 mL	0.2739 mL	0.5478 mL	1.3695 mL
	80 mM	0.0411 mL	0.2054 mL	0.4108 mL	1.0271 mL
	100 mM	0.0329 mL	0.1643 mL	0.3287 mL	0.8217 mL

Taxifolin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Taxifolin480-18-2(+)-Dihydroquercetin (+)-TaxifolinTNF ReceptorAutophagyTyrosinaseTumor Necrosis Factor ReceptorTNFRantifibroticantioxidantInhibitorinhibitorinhibit

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Taxifolin (Synonyms: (+)-Dihydroquercetin; (+)-Taxifolin)

Customer Review

Other Forms of Taxifolin:

Taxifolin Related Antibodies

27 Publications Citing Use of MCE Taxifolin

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View All TNF Receptor Isoform Specific Products:

Biological Activity

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References

Customer Review

Taxifolin Related Antibodies

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Complete Stock Solution Preparation Table

Taxifolin Related Classifications

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