(±)-Taxifolin
Based on 1 Customer Validation
(±)-Taxifolin ((±)-Dihydroquercetin) is the racemate of Taxifolin (HY-N0136). Taxifolin exhibits important anti-tyrosinase activity. Taxifolin exhibits significant inhibitory activity against collagenase with an IC50 value of 193.3 μM. Taxifolin is an important natural compound with antifibrotic activity. Taxifolin is a free radical scavenger with antioxidant capacity.
For research use only. We do not sell to patients.
- Purity: 99.34%
- CAS No.: 24198-97-8
- Formula: C15H12O7
- Molecular Weight:304.25
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Immunology/Inflammation Compound Library
HY-L007
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Metabolism/Protease Compound Library
HY-L012
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Natural Product Library
HY-L021
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Anti-Cancer Compound Library
HY-L025
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Autophagy Compound Library
HY-L029
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Antioxidant Compound Library
HY-L037
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Phenols Library
HY-L057
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Flavonoids Library
HY-L068
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Angiogenesis-Related Compound Library
HY-L088
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Anti-Cancer Natural Product Library
HY-L107
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Plant-Sourced Natural Product Library
HY-L115
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Coagulation and Anti-coagulation Compound Library
HY-L136
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Metabolic Enzyme Compound Library
HY-L146
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Membrane Protein-targeted Compound Library
HY-L149
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Membrane Receptor-targeted Compound Library
HY-L150
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Autoimmune Disease Compound Library
HY-L156
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Cell Death Library
HY-L162
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Multi-Target Compound Library
HY-L176
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Radioprotector Library
HY-L178
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Bioactive Compound Library Max
HY-L181
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Natural Product Library Plus
HY-L021P
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Natural Product and Natural Product-Like Compound Library
HY-L021M
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Bioactive Compound Library
HY-L001
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Anti-Fibrosis Compound Library
HY-L185
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Anti-Brain Cancer Compound Library
HY-L188
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RO5 Drug-like Natural Product Library
HY-L200
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High-Throughput Bioactive Compound Library
HY-L205
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High-Throughput Natural Product Library
HY-L206
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Mass Spectrometry Natural Product Library
HY-L262
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| BJ | EC50 |
>10 μM
Compound: 1
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Cytotoxicity against human BJ cells assessed as viable cells after 72 hrs by calcein AM assay
Cytotoxicity against human BJ cells assessed as viable cells after 72 hrs by calcein AM assay
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[PMID: 20192247] |
| CCRF-CEM | EC50 |
>10 μM
Compound: 1
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Cytotoxicity against human CEM cells assessed as viable cells after 72 hrs by calcein AM assay
Cytotoxicity against human CEM cells assessed as viable cells after 72 hrs by calcein AM assay
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[PMID: 20192247] |
| HEK293 | EC50 |
670.66 μM
Compound: DHQ
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Cytotoxicity in HEK293 cells assessed as reduction in cell survival incubated for 48 hrs by MTT assay
Cytotoxicity in HEK293 cells assessed as reduction in cell survival incubated for 48 hrs by MTT assay
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[PMID: 30108751] |
| HeLa | EC50 |
>10 μM
Compound: 1
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Cytotoxicity against human HeLa cells assessed as viable cells after 72 hrs by calcein AM assay
Cytotoxicity against human HeLa cells assessed as viable cells after 72 hrs by calcein AM assay
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[PMID: 20192247] |
| HeLa | EC50 |
125.31 μM
Compound: DHQ
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Cytotoxicity in human HeLa cells assessed as reduction in cell survival incubated for 48 hrs by MTT assay
Cytotoxicity in human HeLa cells assessed as reduction in cell survival incubated for 48 hrs by MTT assay
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[PMID: 30108751] |
| HL-60 | IC50 |
10 ng/mL
Compound: Taxifolin
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Growth inhibition of human HL60 cells after 72 hrs by MTT assay
Growth inhibition of human HL60 cells after 72 hrs by MTT assay
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[PMID: 31398616] |
| L929 | EC50 |
200 μM
Compound: taxifolin
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Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by crystal violet staining
Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by crystal violet staining
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[PMID: 9287415] |
| L929 | EC50 |
240 μM
Compound: taxifolin
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Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay
Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay
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[PMID: 9287415] |
| MCF7 | EC50 |
>10 μM
Compound: 1
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Cytotoxicity against human MCF7 cells assessed as viable cells after 72 hrs by calcein AM assay
Cytotoxicity against human MCF7 cells assessed as viable cells after 72 hrs by calcein AM assay
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[PMID: 20192247] |
| MT4 | CC50 |
25.4 μg/mL
Compound: 14
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Cytotoxicity against human MT4 cells by MTT assay
Cytotoxicity against human MT4 cells by MTT assay
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[PMID: 10425115] |
| MT4 | IC50 |
>25.4 μg/mL
Compound: 14
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Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay
Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay
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[PMID: 10425115] |
| Neutrophil | IC50 |
15.3 μM
Compound: 5c
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Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of HOCl-induced oxidation of APF after 6 mins by fluorescence assay
Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of HOCl-induced oxidation of APF after 6 mins by fluorescence assay
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[PMID: 23871908] |
| Neutrophil | IC50 |
15.5 μM
Compound: 5c
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Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of superoxide anion radical-induced lucigenin oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay
Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of superoxide anion radical-induced lucigenin oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay
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[PMID: 23871908] |
| Neutrophil | IC50 |
41.3 μM
Compound: 5c
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Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of H2O2-induced oxidation of amplex red incubated for 5 mins prior to PMA challenge by fluorescence assay
Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of H2O2-induced oxidation of amplex red incubated for 5 mins prior to PMA challenge by fluorescence assay
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[PMID: 23871908] |
| Neutrophil | IC50 |
53.5 μM
Compound: 5c
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Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of ROS-induced luminol oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay
Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of ROS-induced luminol oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay
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[PMID: 23871908] |
| RAW264.7 | IC50 |
81.6 μM
Compound: 10
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Inhibition of LPS-induced NO production in mouse RAW264.7 cells by Griess reagent based assay
Inhibition of LPS-induced NO production in mouse RAW264.7 cells by Griess reagent based assay
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[PMID: 32118424] |
| RPMI-8226 | EC50 |
>10 μM
Compound: 1
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Cytotoxicity against human RPMI8226 cells assessed as viable cells after 72 hrs by calcein AM assay
Cytotoxicity against human RPMI8226 cells assessed as viable cells after 72 hrs by calcein AM assay
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[PMID: 20192247] |
| Sf9 | IC50 |
15.7 μM
Compound: 4
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Inhibition of N-terminal GST-His-tagged c-KIT (544 to 976 amino acids) D816V mutant (unknown origin) expressed in Sf9 insect cells using poly[Glu:Tyr] (4:1) as substrate preincubated for 20 mins followed by [33P-gamma]ATP addition and subsequent inhibitio
Inhibition of N-terminal GST-His-tagged c-KIT (544 to 976 amino acids) D816V mutant (unknown origin) expressed in Sf9 insect cells using poly[Glu:Tyr] (4:1) as substrate preincubated for 20 mins followed by [33P-gamma]ATP addition and subsequent inhibitio
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[PMID: 26807861] |
| THP-1 | EC50 |
>10 μM
Compound: 1
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Cytotoxicity against human THP1 cells after 72 hrs by erythosin B staining method
Cytotoxicity against human THP1 cells after 72 hrs by erythosin B staining method
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[PMID: 20192247] |
| U-266 | EC50 |
>10 μM
Compound: 1
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Cytotoxicity against human U266 cells assessed as viable cells after 72 hrs by calcein AM assay
Cytotoxicity against human U266 cells assessed as viable cells after 72 hrs by calcein AM assay
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[PMID: 20192247] |
Taxifolin (HY-N0136) exhibits important anti-tyrosinase activity. Taxifolin exhibits significant inhibitory activity against collagenase with an IC50 value of 193.3 μM. Taxifolin is an important natural compound with antifibrotic activity. Taxifolin is a free radical scavenger with antioxidant capacity[1][2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 24198-97-8
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Appearance Solid
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Molecular Weight 304.25
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Formula C15H12O7
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Color White to off-white
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SMILES
O=C1[C@H](O)[C@@H](C2=CC=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C13
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Synonyms
(±)-Dihydroquercetin
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (328.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.22 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.22 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. Angelis A, et al. Bio-Guided Isolation of Methanol-Soluble Metabolites of Common Spruce (Picea abies) Bark by-Products and Investigation of Their Dermo-Cosmetic Properties. Molecules. 2016 Nov 21;21(11):1586. [Content Brief]
[2]. Lei Ren, et al. Dissecting Efficacy and Metabolic Characteristic Mechanism of Taxifolin on Renal Fibrosis by Multivariate Approach and Ultra-Performance Liquid Chromatography Coupled With Mass Spectrometry-Based Metabolomics Strategy. Front Pharmacol. 2021 Jan 14;11:608511. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2868 mL | 16.4339 mL | 32.8677 mL | 82.1693 mL |
| 5 mM | 0.6574 mL | 3.2868 mL | 6.5735 mL | 16.4339 mL | |
| 10 mM | 0.3287 mL | 1.6434 mL | 3.2868 mL | 8.2169 mL | |
| 15 mM | 0.2191 mL | 1.0956 mL | 2.1912 mL | 5.4780 mL | |
| 20 mM | 0.1643 mL | 0.8217 mL | 1.6434 mL | 4.1085 mL | |
| 25 mM | 0.1315 mL | 0.6574 mL | 1.3147 mL | 3.2868 mL | |
| 30 mM | 0.1096 mL | 0.5478 mL | 1.0956 mL | 2.7390 mL | |
| 40 mM | 0.0822 mL | 0.4108 mL | 0.8217 mL | 2.0542 mL | |
| 50 mM | 0.0657 mL | 0.3287 mL | 0.6574 mL | 1.6434 mL | |
| 60 mM | 0.0548 mL | 0.2739 mL | 0.5478 mL | 1.3695 mL | |
| 80 mM | 0.0411 mL | 0.2054 mL | 0.4108 mL | 1.0271 mL | |
| 100 mM | 0.0329 mL | 0.1643 mL | 0.3287 mL | 0.8217 mL |
(±)-Taxifolin Related Classifications
HY-N0136A Related Classifications
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