[Ac-Tyr1,D-Phe2]GRF 1-29, amide (human)
[Ac-Tyr1,D-Phe2]GRF 1-29, amide (human), a growth hormone releasing factor (GRF) analogue, is a vasoactive intestinal peptide (VIP) antagonist.
For research use only. We do not sell to patients.
- CAS No.: 93965-89-0
- Formula: C157H252N44O43S
- Molecular Weight:3476.01
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
![[Ac-Tyr1,D-Phe2]GRF 1-29, amide (human) Chemical Structure](https://file.medchemexpress.com/product_pic/hy-p0211.gif "Click to View [Ac-Tyr1,D-Phe2]GRF 1-29, amide (human) Chemical Structure")
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
![[Ala2,8,9,11,19,22,24,25,27,28]-VIP](https://file.medchemexpress.com/product_pic/hy-p1158.gif)
Biological Activity
VIP[1]
[Ac-Tyr1,D-Phe2]GRF 1-29, amide (human) selectively inhibits both VIP and GRF-stimulated adenylate cyclase activities in rat pancreatic plasma membranes[1].
[Ac-Tyr1,D-Phe2]GRF 1-29, amide (human) (0.1 pM-10 nM; 3 d) induces a dose-dependent cell proliferation increase in C6 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:C6 rat glioblastoma cells
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Concentration:0.1 pM-10 nM
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Incubation Time:3 days
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Result:Induced a dose-dependent cell proliferation increase.
Chemical Information
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CAS No. 93965-89-0
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Molecular Weight 3476.01
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Formula C157H252N44O43S
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Sequence
Ac-Tyr-{d-Phe}-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-NH2
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Sequence Shortening
Ac-Y-{d-Phe}-DAIFTNSYRKVLGQLSARKLLQDIMSR-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
| Contents | Assign value | |
|---|---|---|
| Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 |
| Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 |
| Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution:
| Overall charge of peptide | Details |
|---|---|
| Negative (<0) |
1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. |
| Positive (>0) |
1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. |
| Zero (=0) |
1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
Purity & Documentation
References
[1]. Waelbroeck M, et al. Interaction of growth hormone-releasing factor (GRF) and 14 GRF analogs with vasoactive intestinal peptide (VIP) receptors of rat pancreas. Discovery of (N-Ac-Tyr1,D-Phe2)-GRF(1-29)-NH2 as a VIP antagonist. Endocrinology. 1985 Jun;116(6):2643-9. [Content Brief]
[2]. Dufes C, et al. Effects of the vasoactive intestinal peptide (VIP) and related peptides on glioblastoma cell growth in vitro. J Mol Neurosci. 2003;21(2):91-102. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
[Ac-Tyr1,D-Phe2]GRF 1-29, amide (human) Related Classifications
HY-P0211 Related Classifications
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