AF488 streptavidin
Based on 1 Customer Validation
AF488 streptavidin is a fluorescently labeled streptavidin. AF488 streptavidin is a streptavidin conjugated to Alexa Fluor 488, with 4 fluorophores per protein molecule, enabling stoichiometric fluorescent labeling of cell surface targets. AF488 streptavidin can form a complex with biotinylated E07 aptamer to stain cells expressing EGFR, and the staining is reversible after treatment with mA9 detoxifying oligonucleotides (Ex/Em = 470/520 nm).
For research use only. We do not sell to patients.
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
AF488 streptavidin (2:1 molar ratio (btE07 to AF488-SA); 20 min at room temperature) forms stable, functional complexes with biotin-tailed-E07 aptamer, enabling EGFR-targeted fluorescent cell labeling[1].
AF488 streptavidin (500 nM AF488-SA-E07; 30 min at 4 °C), as part of AF488-SA-E07 complexes, enables specific fluorescent staining of EGFR-positive A431 cells, and the AF488 signal can be reversed with mA9 antidote to allow subsequent restaining of cells with alternative SA-aptamer complexes[1].
AF488 streptavidin (15 min) produces lower total fluorescence signal when labeling EGFR on live A431 cells than the laccase-mediated CARD approach using AF488-FA[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Color Yellow to orange
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SMILES
[AF488 streptavidin]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Purity & Documentation
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Data Sheet (266 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Gray BP, et al. Aptamers as Reversible Sorting Ligands for Preparation of Cells in Their Native State. Cell Chem Biol. 2020;27(2):232-244.e7. [Content Brief]
[2]. Cisneros BT, et al. Laccase-Mediated Catalyzed Fluorescent Reporter Deposition for Live-Cell Imaging. Chembiochem. 2020;21(1-2):98-102. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
AF488 streptavidin Related Classifications
HY-D2170 Related Classifications
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