CEP63 Antibody (YA8767)

Cat. No.: HY-P89083: Data Sheet User Guide for Antibodies
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CEP63 Antibody (YA8767) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CEP63.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

CEP63 Antibody (YA8767) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CEP63.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 62.8 kDa

Species Reactivity

Human

SwissProt ID

Q96MT8

Immunogen

Full length human recombinant protein of human CEP63 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500-800

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Required for normal spindle assembly (PubMed:21406398, PubMed:21983783, PubMed:26297806, PubMed:35793002). Plays a key role in mother-centriole-dependent centriole duplication; the function seems also to involve CEP152, CDK5RAP2 and WDR62 through a stepwise assembled complex at the centrosome that recruits CDK2 required for centriole duplication (PubMed:21983783, PubMed:26297806). Reported to be required for centrosomal recruitment of CEP152; however, this function has been questioned (PubMed:21983783, PubMed:26297806). Also recruits CDK1 to centrosomes (PubMed:21406398). Plays a role in DNA damage response (PubMed:21406398). Following DNA damage, such as double-strand breaks (DSBs), is removed from centrosomes; this leads to the inactivation of spindle assembly and delay in mitotic progression (PubMed:21406398). Promotes stabilization of FXR1 protein by inhibiting FXR1 ubiquitination (PubMed:35989368)

Subcellular Localization：Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite

Isoforms & Post-Translational Modification：Q96MT8 has four isomers: Q96MT8-1: 81344 Da (predicted); Q96MT8-2: 63015 Da (predicted); Q96MT8-3: 57908 Da (predicted); Q96MT8-4: 55591 Da (predicted).
Polyubiquitinated via 'Lys-48'-linked ubiquitin, leading to its degradation (PubMed:35989368)

Subunit：Interacts with CEP152 and CDK1; these interactions recruit both ligands to centrosomes (PubMed:21406398)

Synonyms

SCKL6

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/astrocyte-cell.html [Content Brief]