CHX10 Antibody (YA9745)
CHX10 Antibody (YA9745) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to CHX10.
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Host:
Rabbit
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Isotype:
IgG
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Application:
IHC-P, IHC-F
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Reactivity :
Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclin950.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-F
IHC-F: Immunohistochemistry-Frozen
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:200-500 | 1:1000-4000 |
Product Details
CHX10 Antibody (YA9745) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to CHX10.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityMouse, Rat
Recombinant protein within mouse CHX10 (aa 1-361).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclin950.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CHX10 acts as a transcriptional regulator through binding to DNA at the consensus sequence 5'-[TC]TAATT[AG][AG]-3' upstream of gene promoters. Plays a significant role in the specification and morphogenesis of the sensory retina. May play a role in specification of V2a interneurons during spinal cord development. Mediates differentiation of V2a interneurons by repression of motor neuron gene transcription, via competitively binding to response elements that are activated by the ISL1-LHX3 complex, such as VSX1 and MNX1. Acts as a positive transcriptional regulator of NXNL1; regulation is significantly increased in synergy with VSX1. Acts as a negative transcriptional regulator of MITF. Represses SAG transcription by competitive inhibition of ISL1-LHX3 response elements (By similarity). Binds to the photoreceptor conserved element-1 (PCE-1) in the promoter of rod photoreceptor arrestin SAG and acts as a transcriptional repressor. Involved in the development of retinal ganglion cells (RGCs) which leads to release of SHH by RGCs, promoting Hedgehog signaling and subsequent proliferation of retinal progenitor cells. Participates in the development of the cells of the inner nuclear layer, by promoting postnatal differentiation of bipolar cells with a comparable inhibition of rod cell differentiation. May play a role in the maintenance of neural retina identity during development by regulation of canonical Wnt genes and CTNNB1 localization, suggesting a role in the regulation of canonical Wnt signaling (By similarity)[1][2][3][4][5][6][7][8][9].
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Subcellular Localization
Nucleus
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Expression
Tissue_Specificity: Expressed in the retina (at protein level). Expressed throughout the anterior optic vesicle and all neuroblasts of the optic cup (at protein level). In the mature and postnatal retina is restricted to the inner nuclear layer, in which its expression decreases from the outer to the inner margin (at protein level). Also detected in regions of the developing thalamus, hindbrain, and ventral spinal cord. -
Isoforms & Post-Translational Modification
CHX10 has an amino acid length of 361, molecular weight is 39436 Da.
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Subunit
Interacts with MITF
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SwissProt ID
Documentation
References
[1]. Clark AM, et al. Negative regulation of Vsx1 by its paralog Chx10/Vsx2 is conserved in the vertebrate retina. Brain Res. 2008 Feb 4;1192:99-113. [Content Brief]
[2]. Lee S, et al. A regulatory network to segregate the identity of neuronal subtypes. Dev Cell. 2008 Jun;14(6):877-89. [Content Brief]
[3]. Burmeister M, et al. Ocular retardation mouse caused by Chx10 homeobox null allele: impaired retinal progenitor proliferation and bipolar cell differentiation. Nat Genet. 1996 Apr;12(4):376-84. [Content Brief]
[4]. Clovis YM, et al. Chx10 Consolidates V2a Interneuron Identity through Two Distinct Gene Repression Modes. Cell Rep. 2016 Aug 9;16(6):1642-1652. [Content Brief]
[5]. Reichman S, et al. The homeobox gene CHX10/VSX2 regulates RdCVF promoter activity in the inner retina. Hum Mol Genet. 2010 Jan 15;19(2):250-61. [Content Brief]
[6]. Zou C, et al. Vsx2 controls eye organogenesis and retinal progenitor identity via homeodomain and non-homeodomain residues required for high affinity DNA binding. PLoS Genet. 2012 Sep;8(9):e1002924. [Content Brief]
[7]. Dorval KM, et al. CHX10 targets a subset of photoreceptor genes. J Biol Chem. 2006 Jan 13;281(2):744-51. [Content Brief]
[8]. Sigulinsky CL, et al. Vsx2/Chx10 ensures the correct timing and magnitude of Hedgehog signaling in the mouse retina. Dev Biol. 2008 May 15;317(2):560-75. [Content Brief]
[9]. Livne-Bar I, et al. Chx10 is required to block photoreceptor differentiation but is dispensable for progenitor proliferation in the postnatal retina. Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):4988-93. [Content Brief]
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