FAIM3 Antibody (YA8367)(PBS only)

Cat. No.: HY-P88683A: Data Sheet User Guide for Antibodies
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FAIM3 Antibody (YA8367) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FAIM3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

FAIM3 Antibody (YA8367) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FAIM3.

Host

Mouse

Molecular Weight

Predicted band size: 43 kDa

Species Reactivity

Human

SwissProt ID

O60667

Immunogen

Full length human recombinant protein of human FAIM3 produced in HEK293 Cells.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：High-affinity Fc receptor for immunoglobulin M (IgM), both secreted and membrane-bound IgM (PubMed:19858324, PubMed:22675200, PubMed:36949194, PubMed:37095205). Primarily regulates IgM transport and homeostasis. In lymphoid cells, enables exocytosis of membrane-bound IgM on the plasma membrane as well as endocytosis of IgM-antigen complexes toward lysosomes for degradation. In mucosal epithelium, mediates retrotranscytosis of antigen-IgM complexes across mucosal M cells toward antigen-presenting cells in mucosal lymphoid tissues (PubMed:21908732, PubMed:28230186). Triggers costimulatory signaling and mediates most of IgM effector functions involved in B cell development and primary immune response to infection. Likely limits tonic IgM BCR signaling to self-antigens for proper negative selection of autoreactive B cells in the bone marrow and for the maintenance of regulatory B cell pool in peripheral lymphoid organs. Mediates antibody responses to T cell-dependent and T cell-independent antigens and promotes induction of an efficient neutralizing IgG response. Engages in cross-talk with antigen-receptor signaling via the non-canonical NF-kappa-B, MAP kinases and calcium signaling pathways (PubMed:19858324, PubMed:22675200, PubMed:25601920, PubMed:30840890)

Subcellular Localization：Cell membrane,Early endosome membrane,Golgi apparatus, trans-Golgi network membrane,Lysosome membrane,Secreted

Expression：
Tissue_Specificity: Expressed by CD19-positive B cells and CD4-positive and CD8-positive T cell populations in primary and secondary lymphoid tissues (at protein level). Among B cell subsets, detected in a subset of bone marrow pro- and pre-B cells, in most follicular and memory B cells and in a small subset of germinal center B cells (at protein level). Expressed at lower levels in CD56-positive NK cells (at protein level) (PubMed:19858324, PubMed:21908732, PubMed:22675200, PubMed:30840890). Expressed in lymph nodes, lung, thymus and kidneys. Very weak expression detected in spleen, liver, heart, and salivary gland
Induction: Regulated by circulating IgM levels and immune cell activation signaling. Down-regulated in activated T cells, effector memory and central memory T cells, as well as in activated NK cells (PubMed:19858324, PubMed:22675200, PubMed:30840890, PubMed:9586636). Transcriptionally down-regulated upon TLR9 signaling (PubMed:21908732). Down-regulated in response to IL7 and IL15 (PubMed:30840890). Down-regulated in old age (PubMed:30840890)

Isoforms & Post-Translational Modification：O60667 has three isomers: O60667-1: 43146 Da (predicted); O60667-2: 31029 Da (predicted); O60667-3: 33828 Da (predicted).
Phosphorylated on both Tyr and Ser residues丨O-glycosylated

Subunit：Interacts (via Ig-like domain) with IGHM (via CH4/Cmu4 domain), both secreted and membrane-bound IgM; the interaction is glycan-independent and multivalent theoretically involving up to eight binding sites for the IgM pentamer

Synonyms

FAIM3; TOSO

Documentation

User Guide for Antibodies (1077 KB)