DNA polymerase iota Antibody (YA8056)
(Synonyms: polymerase (DNA) iota, Eta2, RAD3OB, RAD30B)POLI Antibody (YA8056) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to POLI.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:1000-4000 |
Product Details
POLI Antibody (YA8056) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to POLI.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 82.8 kDa
Human recombinant protein fragment corresponding to amino acids 458-740 of human POLI produced in E.coli.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
DNA polymerase iota is an Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity[1][2][3][4][5][6][7].
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Subcellular Localization
Nucleus
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Expression
Tissue_Specificity: Ubiquitous. Highly expressed in testis -
Isoforms & Post-Translational Modification
Q9UNA4: 740 amino acids, molecular weight 83006 Da.
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Subunit
Interacts with POLH
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SwissProt ID
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Synonyms
polymerase (DNA) iota, Eta2, RAD3OB, RAD30B
Documentation
References
[1]. Tissier A, et al. Misinsertion and bypass of thymine-thymine dimers by human DNA polymerase iota. EMBO J. 2000 Oct 2;19(19):5259-66. [Content Brief]
[2]. Frank EG, et al. Altered nucleotide misinsertion fidelity associated with poliota-dependent replication at the end of a DNA template. EMBO J. 2001 Jun 1;20(11):2914-22. [Content Brief]
[3]. Haracska L, et al. A mechanism for the exclusion of low-fidelity human Y-family DNA polymerases from base excision repair. Genes Dev. 2003 Nov 15;17(22):2777-85. [Content Brief]
[4]. Washington MT, et al. Efficient and error-free replication past a minor-groove DNA adduct by the sequential action of human DNA polymerases iota and kappa. Mol Cell Biol. 2004 Jul;24(13):5687-93. [Content Brief]
[5]. Nair DT, et al. Replication by human DNA polymerase-iota occurs by Hoogsteen base-pairing. Nature. 2004 Jul 15;430(6997):377-80. [Content Brief]
[6]. Faili A, et al. Induction of somatic hypermutation in immunoglobulin genes is dependent on DNA polymerase iota. Nature. 2002 Oct 31;419(6910):944-7. [Content Brief]
[7]. Bebenek K, et al. 5'-Deoxyribose phosphate lyase activity of human DNA polymerase iota in vitro. Science. 2001 Mar 16;291(5511):2156-9. [Content Brief]
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