Art. -Nr.: HY-B1192

Estradiol benzoate

(Synonyms: β-Estradiol 3-benzoate; 17β-Estradiol 3-benzoate)

22 Cited Publications

Kundenbewertung

Based on 22 publication(s) in Google Scholar

Estradiol benzoate (β-Estradiol 3-benzoate) is a HBx protein inhibitor and inhibits androgen and hepatitis B virus (HBV) transcription, replication. Estradiol benzoate shows antifertility effects, anti- Toxoplasma gondii activity and can improve memory behavior of Ovariectomy (Ovx) female mice.

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Reinheit: 99.90%
CAS. Nr.: 50-50-0
Formel: C₂₅H₂₈O₃
Molecular Weight:376.49

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Estradiol benzoate Chemical Structure

CAS. Nr.: 50-50-0

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (22)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

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Alle Estrogen Receptor/ERR Isoform-spezifische Produkte anzeigen :

Alle Bcl-2 Family Isoform-spezifische Produkte anzeigen :

In Vitro

Estradiol benzoate (5-75 μM, 24 h-48 h) has a downregulation effect on HBV translation, transcription, and replication in SBHX21 cells^[3].
Estradiol benzoate (0-500 μM, 72 h) has toxicity to Human foreskin fibroblasts (HFFs)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[4]

Cell Line:

HFFs
Concentration:

0-500 μM
Incubation Time:

72 h
Result:

Inhibited HFFs at the 50% toxicity of 34.11 μM.

In Vivo

Estradiol benzoate (20-100 μg/kg, Subcutaneous injection, once a day for 4-5 weeks) improves learning and memory behavior of Ovx female mice^[1].
Estradiol benzoate (0.015-15000 μg/kg, Subcutaneous injection, single dose) advances male puberty in male Sprague-Dawley (SD) rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Ovx mice^[1]
Dosage:

20, 100 or 200 μg/kg
Administration:

Subcutaneous injection (s.c.)
Result:

Significantly reversed the lowered uterine weight induced by Ovx.
Had no obvious effects on locomotor activity in Ovx mice.
Effectively reversed the changes of enlarging the width of synaptic cleft and reducing the thickness of PSD induced by Ovx.
Increased the number and density of synaptic vesicles by 4-week replacement with EB (100 μg/kg).

Chemical Information

CAS. Nr. 50-50-0
Appearance Solid
Molecular Weight 376.49
Formel C₂₅H₂₈O₃
Color White to off-white
SMILES

C[C@@]12[C@@H](O)CC[C@@]1([H])[C@]3([H])CCC4=C(C=CC(OC(C5=CC=CC=C5)=O)=C4)[C@@]3([H])CC2
Synonyms

β-Estradiol 3-benzoate; 17β-Estradiol 3-benzoate
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (22)

All In Vivo Efficacy StudyHistological Imaging/Staining

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Journal Impact Factor

ACS Nano

Clinically Validated Leptin Receptor as a Target for Liposomal Delivery of Metformin in Endometriosis Therapy. [Abstract]

2026 May 12;20(18):13542-13559. PMID: 42054534
Redox Biol

Stromal cell-derived itaconate promotes endometriosis via macrophage NRF2 and lysosomal pH modulation. [Abstract]

2026 May:92:104101. PMID: 41780193
J Nanobiotechnology

Highly specific neutrophil-mediated delivery of albumin nanoparticles to ectopic lesion for endometriosis therapy. [Abstract]

2023 Mar 8;21(1):81. PMID: 36890521

Estradiol benzoate purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Mar 8;21(1):81. [Abstract]

Estradiol benzoate (3 μg) induced endometriosis-like lesions in diverse peritoneal locations of female mice.
NPJ Biofilms Microbiomes

Parabacteroides goldsteinii and its metabolite 7-KLCA attenuate endometriosis via TGR5 to reprogram macrophages by modulating the PPARγ/GPR132 axis. [Abstract]

2026 May 22. PMID: 42173876
Phytomedicine

Cryptotanshinone alleviates immunosuppression in endometriosis by targeting MDSCs through JAK2/STAT3 pathway. [Abstract]

2024 Nov 14:136:156227. PMID: 39580997

Estradiol benzoate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Nov 14:136:156227. [Abstract]

Estradiol benzoate (0.3 μg; i.m.;every three days). H&E staining of the vehicle group revealed endometrial epithelial cells and stromal cells within the endometriotic lesions, along with glandular hyperplasia in the lamina propria.

Estradiol benzoate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Nov 14:136:156227. [Abstract]

Morphology of endometriotic lesions excised from the peritoneum of mice. Estradiol benzoate (0.3 μg; i.m.;every three days) successfully induced EMS in mice.
Anal Chem

High-Throughput Reactive Desorption Electrospray Ionization Mass Spectrometry for Targeted Derivatization and Analysis of Poorly Ionized Small Molecules. [Abstract]

2026 Jun 16;98(23):16962-16970. PMID: 42223360
J Med Chem

Rational Design of a Novel 6 H-Benzo[ c]chromen Series as Selective PI3Kα Inhibitors. [Abstract]

2024 Sep 12;67(17):15387-15410. PMID: 39159497

Estradiol benzoate purchased from MedChemExpress. Usage Cited in: J Med Chem. 2024 Sep 12;67(17):15387-15410. [Abstract]

Estradiol benzoate (17β-Estradiol) (1 μM; drinking water) was used to sustain the MCF7 xenograft model in NCG mice.
J Ethnopharmacol

An experimental study of the anti-dysmenorrhea effect of Chinese herbal medicines used in Jin Gui Yao Lue. [Abstract]

2019 Dec 5:245:112181. PMID: 31446072

Estradiol benzoate purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2019 Dec 5:245:112181. [Abstract]

ICR female mice were treated with Estradiol benzoate (1 mg/kg; i.p.; 3 d). On the fourth day, the mice were injected with oxytocin (50 U/kg) to induce dysmenorrhea.
Commun Biol

Diurnal testosterone oscillations gate drug delivery via phase separation of ZO-1 at the blood-testis barrier in mice. [Abstract]

2026 Jun 1. PMID: 42219451
Front Cell Dev Biol

Comprehensive Analysis of Quantitative Proteomics With DIA Mass Spectrometry and ceRNA Network in Intrahepatic Cholestasis of Pregnancy. [Abstract]

2022 Jul 22:10:854425. PMID: 35938169
Bioorg Chem

Dehydroepiandrosterone ameliorates primary dysmenorrhea by suppressing the SP1/Hsp90ab1/COX-2 signaling pathway. [Abstract]

2025 Feb 1:156:108235. PMID: 39914036
Molecules

Identification of Estradiol Benzoate as an Inhibitor of HBx Using Inducible Stably Transfected HepG2 Cells Expressing HiBiT Tagged HBx. [Abstract]

2022 Aug 6;27(15):5000. PMID: 35956950
Mol Med Rep

Upregulation of miR‑33b promotes endometriosis via inhibition of Wnt/β‑catenin signaling and ZEB1 expression. [Abstract]

2019 Mar;19(3):2144-2152. PMID: 30664209
iScience

Analysis of microbiota reveals the underlying mechanism of PHF11 in the development of Enterococcus-regulated endometriotic cysts. [Abstract]

2023 Oct 7;26(11):108158. PMID: 37915596
Reprod Biomed Online

Blocking sphingosine 1-phosphate receptor 1 with modulators reduces immune cells infiltration and alleviates endometriosis in mice. [Abstract]

2023 Nov;47(5):103304. PMID: 37757611
Mol Immunol

Puerarin and DHEA combination therapy alleviates primary dysmenorrhea via inhibition of the Hsp90ab1/p38/JNK pathway. [Abstract]

2026 Jun:194:94-103. PMID: 42030694
J Pharmacol Sci

Activation of σ-1 receptor mitigates estrogen withdrawal-induced anxiety/depressive-like behavior in mice via restoration of GABA/glutamate signaling and neuroplasticity in the hippocampus. [Abstract]

2024 Apr;154(4):236-245. PMID: 38485341
Am J Transl Res

Curcumin treats endometriosis in mice by the HIF signaling pathway. [Abstract]

2022 Apr 15;14(4):2184-2198. PMID: 35559378
Res Sq

2026 Jun 2.
SSRN

2023 Jun 19.
Evid Based Complement Alternat Med

Huayu Jiedu Fang Protects Ovarian Function in Mouse with Endometriosis Iron Overload by Inhibiting Ferroptosis. [Abstract]

2022 Aug 30;2022:1406820. PMID: 36082180
Trop J Pharm Res

2019; 18 (11): 2349.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (132.81 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 3.33 mg/mL (8.84 mM; Need ultrasonic)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6561 mL

13.2806 mL

26.5611 mL

5 mM

0.5312 mL

2.6561 mL

5.3122 mL

10 mM

0.2656 mL

1.3281 mL

2.6561 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Xu X, et al. Effects of estradiol benzoate on learning-memory behavior and synaptic structure in ovariectomized mice [J]. Life sciences, 2006, 79(16): 1553-1560.

[2]. Putz O, et al. Neonatal low-and high-dose exposure to estradiol benzoate in the male rat: I. Effects on the prostate gland [J]. Biology of reproduction, 2001, 65(5): 1496-1505.

[3]. He J, et al. Identification of Estradiol Benzoate as an Inhibitor of HBx Using Inducible Stably Transfected HepG2 Cells Expressing HiBiT Tagged HBx [J]. Molecules, 2022, 27(15): 5000.

[4]. Lu D, et al. Investigation of Antiparasitic Activity of Two Marine Natural Products, Estradiol Benzoate, and Octyl Gallate, on Toxoplasma gondii In Vitro [J]. Frontiers in Pharmacology, 2022, 13: 841941.

[5]. Rao M V, et al. Effect of estradiol benzoate on reproductive organs and fertility in the male rat [J]. European Journal of Obstetrics & Gynecology and Reproductive Biology, 1983, 15(3): 189-198.

[6]. Zovko M, et al. Macromolecular prodrugs XI. Synthesis and characterization of polymer-estradiol conjugate. Int J Pharm. 2004 Nov 5;285(1-2):35-41. [Content Brief]

[7]. García-Gómez E, et al. Role of sex steroid hormones in bacterial-host interactions. Biomed Res Int. 2013;2013:928290. [Content Brief]

[8]. Estradiol benzoate

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol / DMSO	1 mM	2.6561 mL	13.2806 mL	26.5611 mL	66.4028 mL
Ethanol / DMSO	5 mM	0.5312 mL	2.6561 mL	5.3122 mL	13.2806 mL
DMSO	10 mM	0.2656 mL	1.3281 mL	2.6561 mL	6.6403 mL
	15 mM	0.1771 mL	0.8854 mL	1.7707 mL	4.4269 mL
	20 mM	0.1328 mL	0.6640 mL	1.3281 mL	3.3201 mL
	25 mM	0.1062 mL	0.5312 mL	1.0624 mL	2.6561 mL
	30 mM	0.0885 mL	0.4427 mL	0.8854 mL	2.2134 mL
	40 mM	0.0664 mL	0.3320 mL	0.6640 mL	1.6601 mL
	50 mM	0.0531 mL	0.2656 mL	0.5312 mL	1.3281 mL
	60 mM	0.0443 mL	0.2213 mL	0.4427 mL	1.1067 mL
	80 mM	0.0332 mL	0.1660 mL	0.3320 mL	0.8300 mL
	100 mM	0.0266 mL	0.1328 mL	0.2656 mL	0.6640 mL

Keywords

Estradiol benzoate

22 Cited Publications

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Estradiol benzoate

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Publications Citing Use of MedChemExpress (MCE) Estradiol benzoate

Alle Estrogen Receptor/ERR Isoform-spezifische Produkte anzeigen

Alle Bcl-2 Family Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (22)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Estradiol benzoate

Anti-Infection Compound Library

Apoptosis Compound Library

FDA-Approved Drug Library

Drug Repurposing Compound Library

Lipid Compound Library

NMPA-Approved Drug Library

FDA Approved & Pharmacopeial Drug Library

Mitochondria-Targeted Compound Library

Human Metabolite Library

Nuclear Receptor Compound Library

Off-patent Drug Library

Membrane Receptor-targeted Compound Library

Multi-Target Compound Library

Mitophagy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Animal Drug Library

Publications Citing Use of MedChemExpress (MCE) Estradiol benzoate

Alle Estrogen Receptor/ERR Isoform-spezifische Produkte anzeigen

Alle Bcl-2 Family Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (22)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Estradiol benzoate Related Classifications

HY-B1192 Related Classifications