Cat. No.: HY-N2908

Atraric acid

(Synonyms: Methyl atrarate)

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Based on 1 Customer Validation

Atraric acid (Methyl atrarate) is a specific androgen receptor (AR) antagonist with anti-inflammatory and anticancer effects. Atraric acid represses the expression of the endogenous prostate specific antigen gene in both LNCaP and C4-2 cells. Atraric acid can also inhibit the synthesis of NO and cytokine, and suppress the MAPK-NFκB signaling pathway. Atraric acid can be used to research prostate diseases and inflammatory diseases.

For research use only. We do not sell to patients.

Purity: 99.97%
CAS No.: 4707-47-5
Formula: C₁₀H₁₂O₄
Molecular Weight:196.20

Storage:

RT, stored under nitrogen.

In solvent -80°C, 1 year , -20°C, 6 months

Atraric acid Chemical Structure

CAS No.: 4707-47-5

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Sample (0.1mg)

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(In Japan)

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

Androgen receptor, NO synthesis, MAPK-NFκB pathway^[1]^[2]

All p38 MAPK Isoforms :

All NF-κB Isoforms :

All NO Synthase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HaCaT	IC₅₀	>5 μM Compound: 2	Antiproliferative activity against human HaCaT cells Antiproliferative activity against human HaCaT cells	[PMID: 10395495]
PMNL	IC₅₀	>60 μM Compound: 2	Inhibition of LTB4 biosynthesis in A-23187-stimulated bovine PMNL cells Inhibition of LTB4 biosynthesis in A-23187-stimulated bovine PMNL cells	[PMID: 10395494]

In Vitro

Atraric acid (10 μM; CV1 cells) represses the transactivation function mediated by Dihydrotestosterone-induced human AR^[1].
Atraric acid (10 μM; PCa cells) inhibits the expression of the PSA gene in both androgen-dependent and androgen-independent PCa cells^[1].
Atraric acid (1-300 μM; 24 h) dose-dependently inhibits pro-inflammatory cytokine, nitric oxide, prostaglandin E2 in LPS-stimulated RAW264.7 cells, but does not influence the cell viability^[2].
Atraric acid (100 and 300 μM; 18 h or 4 h) downregulates the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

RAW264.7 cells
Concentration:

1-300 μM
Incubation Time:

24 h
Result:

Did not influence the cell viability.

Western Blot Analysis^[2]

Cell Line:

RAW264.7 cells
Concentration:

100 and 300 μM
Incubation Time:

18 h or 4 h
Result:

Inhibited LPS-Induced expression of iNOS and COX-2 in a dose-dependent manner.
Suppressed LPS-stimulated phosphorylation of the Nfκb signaling pathway.

In Vivo

Atraric acid (10, 30 mg/kg; i.p.; single dosage) inhibits the production of pro-inflammatory cytokines and reduces pathological damages in LPS-induced endotoxin shock mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female BALB/c mice (7 weeks old, 17-20 g; LPS-induced endotoxin shock)^[2]
Dosage:

10, 30 mg/kg
Administration:

i.p.; single dosage
Result:

Inhibited the production of pro-inflammatory cytokines.
Reduced pathological damages such as vasodilation and bleeding.

Chemical Information

CAS No. 4707-47-5
Appearance Solid
Molecular Weight 196.20
Formula C₁₀H₁₂O₄
Color White to off-white
SMILES

O=C(OC)C1=C(C)C=C(O)C(C)=C1O
Synonyms

Methyl atrarate
Structure Classification
- Phenols
Initial Source
- Plants
- Microorganisms
Stereocaulon japonicum
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, stored under nitrogen

In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (509.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.0968 mL

25.4842 mL

50.9684 mL

5 mM

1.0194 mL

5.0968 mL

10.1937 mL

10 mM

0.5097 mL

2.5484 mL

5.0968 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (12.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Papaioannou M, et al. The natural compound atraric acid is an antagonist of the human androgen receptor inhibiting cellular invasiveness and prostate cancer cell growth. J Cell Mol Med. 2009 Aug;13(8B):2210-2223. [Content Brief]

[2]. Roell D, Baniahmad A. The natural compounds atraric acid and N-butylbenzene-sulfonamide as antagonists of the human androgen receptor and inhibitors of prostate cancer cell growth. Mol Cell Endocrinol. 2011 Jan 30;332(1-2):1-8. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.0968 mL	25.4842 mL	50.9684 mL	127.4210 mL
	5 mM	1.0194 mL	5.0968 mL	10.1937 mL	25.4842 mL
	10 mM	0.5097 mL	2.5484 mL	5.0968 mL	12.7421 mL
	15 mM	0.3398 mL	1.6989 mL	3.3979 mL	8.4947 mL
	20 mM	0.2548 mL	1.2742 mL	2.5484 mL	6.3711 mL
	25 mM	0.2039 mL	1.0194 mL	2.0387 mL	5.0968 mL
	30 mM	0.1699 mL	0.8495 mL	1.6989 mL	4.2474 mL
	40 mM	0.1274 mL	0.6371 mL	1.2742 mL	3.1855 mL
	50 mM	0.1019 mL	0.5097 mL	1.0194 mL	2.5484 mL
	60 mM	0.0849 mL	0.4247 mL	0.8495 mL	2.1237 mL
	80 mM	0.0637 mL	0.3186 mL	0.6371 mL	1.5928 mL
	100 mM	0.0510 mL	0.2548 mL	0.5097 mL	1.2742 mL

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Atraric acid

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All p38 MAPK Isoforms

All NF-κB Isoforms

All NO Synthase Isoforms

Biological Activity

Chemical Information

Solvent & Solubility