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Cat. No.: HY-D3603

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Benzylguanine-CY5

Benzylguanine-CY5 is a quenched fluorescent dye conjugate based on benzylguanine (BG). Benzylguanine-CY5 possesses the activity of specifically covalently labeling SNAP-tag fusion proteins. Benzylguanine-CY5 exhibits intramolecular fluorescence quenching in the unbound state; upon binding to SNAP-tag, the quenching guanine moiety is cleaved, and its fluorescence intensity increases. Benzylguanine-CY5 can be used for background-free specific fluorescent labeling and imaging studies of SNAP-tag fusion proteins in living cells.

For research use only. We do not sell to patients.

CAS No.: 928817-42-9
Formula: C₄₆H₅₂N₈O₈S₂
Molecular Weight:909.08

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Please store the product under the recommended conditions in the Certificate of Analysis.

Benzylguanine-CY5 Chemical Structure

CAS No.: 928817-42-9

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
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Biological Activity

Description

In Vitro

Benzylguanine-CY5 (1 μM; 30 min) labels E. coli cells expressing SNAP-tag but causes strong background fluorescence, with a relative fluorescence intensity of 780^[1].
Benzylguanine-CY5 (10 nM; 5 h) increases the fluorescence emission intensity in purified SNAP-tag-containing protein, with a fluorescence efficiency ratio of 1.50^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 928817-42-9
Molecular Weight 909.08
Formula C₄₆H₅₂N₈O₈S₂
SMILES

O=C(CCCCCN1C2=CC=C(S(=O)(O)=O)C=C2C(C)(C)/C1=C\C=C\C=C\C3=[N+](CC)C4=C(C3(C)C)C=C(S(=O)([O-])=O)C=C4)NCC5=CC=C(COC6=NC(N)=NC7=C6NC=N7)C=C5
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Stöhr K, et al. Quenched substrates for live-cell labeling of SNAP-tagged fusion proteins with improved fluorescent background. Analytical chemistry. 2010 Oct 01;82(19):8186-93.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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