1. PI3K/Akt/mTOR Metabolic Enzyme/Protease Anti-infection Vitamin D Related/Nuclear Receptor
  2. Drug Metabolite Akt HSV Androgen Receptor Bacterial
  3. Brassicasterol

Brassicasterol is a metabolite of Ergosterol and has cardiovascular protective effects. Brassicasterol exerts anticancer effects in prostate cancer through dual targeting of AKT and androgen receptor signaling pathways. Brassicasterol inhibits HSV-1 (IC50=1.2 μM) and Mycobacterium tuberculosis. Brassicasterol also inhibits sterol δ 24-reductase, slowing the progression of atherosclerosis. Brassicasterol is also a cerebrospinal fluid biomarker for Alzheimer's disease.

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Brassicasterol Chemical Structure

Brassicasterol Chemical Structure

CAS No. : 474-67-9

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1 mg USD 95 In-stock
5 mg USD 330 In-stock
10 mg USD 460 In-stock
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Description

Brassicasterol is a metabolite of Ergosterol and has cardiovascular protective effects. Brassicasterol exerts anticancer effects in prostate cancer through dual targeting of AKT and androgen receptor signaling pathways. Brassicasterol inhibits HSV-1 (IC50=1.2 μM) and Mycobacterium tuberculosis. Brassicasterol also inhibits sterol δ 24-reductase, slowing the progression of atherosclerosis. Brassicasterol is also a cerebrospinal fluid biomarker for Alzheimer's disease[1][2][3][4][5][6].

IC50 & Target[2]

HSV-1

1.2 μM (IC50)

In Vitro

Brassicasterol (10 μM; 24 h) downregulates dihydrotestosterone (DHT)-induced expression of androgen receptor (AR) and prostate-specific antigen (PSA) in LNCaP cells [4].
Brassicasterol (50 μM; 48 h) Arrests the cell cycle of LNCaP in the sub-G1 phase and induces cell apoptosis [4].
Brassicasterol (10 μM; 48 h) also inhibits LNCaP cell migration [4].
Brassicasterol (100 μg /mL; 48 h) In a 3D organoid model, it can inhibit AKT, and its inhibitory effect on AR-independent cancers and AR-dependent cell[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Migration Assay [4]

Cell Line: LNCaP cells
Concentration: 0, 10, and 50 μM
Incubation Time: 5 days
Result: Showed 54% cell migration inhibitory effect.
Molecular Weight

398.66

Formula

C28H46O

CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

C[C@H](C(C)C)/C=C/[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C

Structure Classification
Initial Source

Hippocampus abdominalis

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

Ethanol : 4.76 mg/mL (11.94 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5084 mL 12.5420 mL 25.0840 mL
5 mM 0.5017 mL 2.5084 mL 5.0168 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% EtOH    90% Corn Oil

    Solubility: ≥ 0.5 mg/mL (1.25 mM); Clear solution

    This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol 1 mM 2.5084 mL 12.5420 mL 25.0840 mL 62.7101 mL
5 mM 0.5017 mL 2.5084 mL 5.0168 mL 12.5420 mL
10 mM 0.2508 mL 1.2542 mL 2.5084 mL 6.2710 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Brassicasterol
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HY-113289
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