Art. -Nr.: HY-N0363

(+)-Columbianetin

(Synonyms: (S)-Columbianetin)

2 Cited Publications

Kundenbewertung

Based on 2 publication(s) in Google Scholar

(+)-Columbianetin ((S)-Columbianetin) acts as an inhibitor of JNK/ERK. (+)-Columbianetin inhibits UVA-induced phosphorylation of JNK and ERK, reduces the production of MMP-1, reverses UVA-induced Collagen (HY-NP003) degradation, and alleviates UVA-mediated inhibition of Smad2/3 phosphorylation and translocation. (+)-Columbianetin regulates the AP-1 and ASK1-MAPK signaling pathways, inhibits the production of ROS and blocks sub-G₁ cell cycle arrest. (+)-Columbianetin is applicable to research related to skin aging.

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Reinheit: 99.04%
CAS. Nr.: 3804-70-4
Formel: C₁₄H₁₄O₄
Molecular Weight:246.26

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

(+)-Columbianetin Chemical Structure

CAS. Nr.: 3804-70-4

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Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle ERK Isoform-spezifische Produkte anzeigen :

Alle JNK Isoform-spezifische Produkte anzeigen :

Alle TGF-beta/Smad Isoform-spezifische Produkte anzeigen :

Smad1
Smad3

Alle p38 MAPK Isoform-spezifische Produkte anzeigen :

In Vitro

(+)-Columbianetin (1-25 μM; 48 h) exhibits dose-dependent cytotoxicity in human dermal fibroblasts with an IC₅₀ of 93.89 μM, and maintains >90% cell viability at concentrations up to 10 μM after 48 h incubation^[1].
(+)-Columbianetin (1-10 μM) dose-dependently suppresses UVA (10 J/cm²)-induced reactive nitrogen species generation in human dermal fibroblasts^[1].
(+)-Columbianetin (1-10 μM; 24 h) dose-dependently reverses UVA (10 J/cm²)-induced changes in human dermal fibroblasts, reducing MMP-1 release to 19,094 pg/mL and increasing type Iα1 pro-collagen release to 1,955 pg/mL at 10 μM after 24 h incubation^[1].
(+)-Columbianetin (1-10 μM; 24 h) dose-dependently reverses UVA (10 J/cm²)-induced changes in human dermal fibroblasts, suppressing MMP-1 gene expression and increasing type I pro-collagen gene expression after 24 h incubation^[1].
(+)-Columbianetin (1-10 μM; 48 h) dose-dependently reverses UVA (10 J/cm²)-induced changes in human dermal fibroblasts, suppressing MMP-1 protein expression and increasing type I pro-collagen and collagen protein expression after 48 h incubation^[1].
(+)-Columbianetin (10 μM; 24 h) suppresses UVA (10 J/cm²)-induced phosphorylation of JNK and ERK, but not p38, in human dermal fibroblasts after 24 h incubation^[1].
(+)-Columbianetin (10 μM; 24 h) reverses UVA (10 J/cm²)-induced changes in the TGFβ pathway in human dermal fibroblasts, increasing p-Smad2/3 and Smad4 protein levels and decreasing Smad7 protein levels after 24 h incubation^[1].
(+)-Columbianetin (10 μM; 24 h) increases nuclear levels of p-Smad2/3 and Smad4, reversing UVA (10 J/cm²)-induced reductions in human dermal fibroblasts after 24 h incubation^[1].
(+)-Columbianetin (1-20 μM; 24 h) dose-dependently increases the viability of UVB-exposed HaCaT cells, with near-full viability restoration at 20 μM after 24 h of treatment^[2].
(+)-Columbianetin (1-20 μM; 24 h) dose-dependently reduces UVB-induced cell injury in HaCaT cells, with LDH release suppressed to ~20% of positive control levels at 20 μM after 24 h of treatment^[2].
(+)-Columbianetin (1-20 μM; 2 h) dose-dependently scavenges H₂O₂-induced intracellular ROS in HaCaT cells, with ROS generation reduced to ~72% of H₂O₂-only levels at 20 μM after 2 h of treatment^[2].
(+)-Columbianetin (1-20 μM; 24 h) dose-dependently scavenges UVB-induced intracellular ROS in HaCaT cells, with ROS generation reduced to ~72% of UVB-only levels at 20 μM after 24 h of treatment^[2].
(+)-Columbianetin (1-20 μM; 24 h) dose-dependently reduces UVB-induced sub-G1 phase cell cycle arrest in HaCaT cells, with sub-G1 phase cell percentage lowered to 10.62% at 20 μM after 24 h of treatment^[2].
(+)-Columbianetin (1-20 μM; 24 h) dose-dependently regulates UVB-altered gene expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 in HaCaT cells after 24 h of treatment^[2].
(+)-Columbianetin (1-20 μM; 24 h) dose-dependently regulates UVB-altered protein expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 in HaCaT cells after 24 h of treatment^[2].
(+)-Columbianetin (1-20 μM; 24 h) dose-dependently inhibits UVB-induced phosphorylation of p38, JNK, and ERK MAPKs in HaCaT cells, with the greatest inhibition observed at 20 μM after 24 h of treatment^[2].
(+)-Columbianetin (1-20 μM; 24 h) dose-dependently inhibits UVB-induced phosphorylation of ASK1 in HaCaT cells, with p-ASK1 levels reduced to ~80% of UVB-only levels at 20 μM after 24 h of treatment^[2].
(+)-Columbianetin (1-20 μM; 24 h) dose-dependently inhibits UVB-induced activation of the AP-1 component c-fos in HaCaT cells, with c-fos levels reduced to ~80% of UVB-only levels at 20 μM after 24 h of treatment^[2].
(+)-Columbianetin (1-20 μM; 24 h) dose-dependently inhibits UVB-induced phosphorylation of p53 and upregulation of Bax in HaCaT cells, with the greatest inhibition observed at 20 μM after 24 h of treatment^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[1]

Cell Line:

UVA-irradiated human dermal fibroblasts (HDFs)
Concentration:

1 μM; 5 μM; 10 μM
Incubation Time:

24 h
Result:

Reduced UVA-increased MMP-1 levels from 20,849 pg/mL to 19,094 pg/mL at 10 μM.
Increased UVA-decreased type Iα1 pro-collagen levels from 1,753 pg/mL to 1,955 pg/mL at 10 μM.

Real Time qPCR^[1]

Cell Line:

UVA-irradiated human dermal fibroblasts (HDFs)
Concentration:

1 μM; 5 μM; 10 μM
Incubation Time:

24 h
Result:

Dose-dependently suppressed UVA-increased MMP-1 mRNA expression.
Dose-dependently increased UVA-decreased type I pro-collagen mRNA expression.

Western Blot Analysis^[1]

Cell Line:

UVA-irradiated human dermal fibroblasts (HDFs)
Concentration:

1 μM; 5 μM; 10 μM
Incubation Time:

48 h
Result:

Dose-dependently suppressed UVA-increased MMP-1 protein expression.
Dose-dependently increased UVA-decreased type I pro-collagen and collagen protein expression.

Western Blot Analysis^[1]

Cell Line:

UVA-irradiated human dermal fibroblasts (HDFs)
Concentration:

10 μM
Incubation Time:

24 h
Result:

Suppressed UVA-induced phosphorylation of JNK and ERK.
Did not significantly affect UVA-induced phosphorylation of p38.\nIncreased UVA-decreased p-Smad2/3 and Smad4 protein levels.
Decreased UVA-increased Smad7 protein levels.\nIncreased nuclear levels of p-Smad2/3 and Smad4, reversing UVA-induced reductions.

RT-PCR^[2]

Cell Line:

UVB-exposed human keratinocyte (HaCaT) cells
Concentration:

1 μM; 5 μM; 20 μM
Incubation Time:

24 h
Result:

Reduced UVB-induced upregulation of MMP-2 and MMP-9 gene expression in a dose-dependent manner.
Inhibited UVB-induced downregulation of TIMP-1 and TIMP-2 gene expression in a dose-dependent manner.

Chemical Information

CAS. Nr. 3804-70-4
Appearance Solid
Molecular Weight 246.26
Formel C₁₄H₁₄O₄
Color Off-white to light yellow
SMILES

O=C1C=CC2=CC=C(O[C@H](C(C)(O)C)C3)C3=C2O1
Synonyms

(S)-Columbianetin
Structure Classification
- Phenylpropanoids
- Coumarins
Initial Source
- Plants
- Umbelliferae
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (2)

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Food Front

2025 Nov 20.
Front Oncol

Network pharmacology combined with molecular docking and experimental validation of the mechanism of action of columbianetin acetate in the treatment of ovarian cancer. [Abstract]

2025 Feb 25:15:1515976. PMID: 40071097

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (406.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.0607 mL

20.3037 mL

40.6075 mL

5 mM

0.8121 mL

4.0607 mL

8.1215 mL

10 mM

0.4061 mL

2.0304 mL

4.0607 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (305 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Jung Hwan Oh, et al. Antiphotoaging Effect of (2′S)-Columbianetin from Corydalis heterocarpa in UVA-Irradiated Human Dermal Fibroblasts. Appl. Sci. 2020, 10(7), 2568.

[2]. Ahn BN, et al. Protective effect of (2'S)-columbianetin from Corydalis heterocarpa on UVB-induced keratinocyte damage. J Photochem Photobiol B. 2012;109:20-27. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.0607 mL	20.3037 mL	40.6075 mL	101.5187 mL
	5 mM	0.8121 mL	4.0607 mL	8.1215 mL	20.3037 mL
	10 mM	0.4061 mL	2.0304 mL	4.0607 mL	10.1519 mL
	15 mM	0.2707 mL	1.3536 mL	2.7072 mL	6.7679 mL
	20 mM	0.2030 mL	1.0152 mL	2.0304 mL	5.0759 mL
	25 mM	0.1624 mL	0.8121 mL	1.6243 mL	4.0607 mL
	30 mM	0.1354 mL	0.6768 mL	1.3536 mL	3.3840 mL
	40 mM	0.1015 mL	0.5076 mL	1.0152 mL	2.5380 mL
	50 mM	0.0812 mL	0.4061 mL	0.8121 mL	2.0304 mL
	60 mM	0.0677 mL	0.3384 mL	0.6768 mL	1.6920 mL
	80 mM	0.0508 mL	0.2538 mL	0.5076 mL	1.2690 mL
	100 mM	0.0406 mL	0.2030 mL	0.4061 mL	1.0152 mL

(+)-Columbianetin

2 Cited Publications

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(+)-Columbianetin

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Publications Citing Use of MedChemExpress (MCE) (+)-Columbianetin

Alle ERK Isoform-spezifische Produkte anzeigen

Alle JNK Isoform-spezifische Produkte anzeigen

Alle TGF-beta/Smad Isoform-spezifische Produkte anzeigen

Alle p38 MAPK Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

(+)-Columbianetin Related Classifications

HY-N0363 Related Classifications