CyGbPF 

CyGbPF is a granzyme B-specific near-infrared fluorescent probe. CyGbPF can be cleaved by granzyme B to remove the peptide cage group, restoring near-infrared fluorescence. CyGbPF passively accumulates in mouse tumors, and its activated fluorescence correlates with granzyme B expression, CD8⁺ cytotoxic T lymphocyte populations, and CD4⁺ helper T lymphocyte populations in tumor tissues. CyGbPF is efficiently cleared by the kidneys, enabling the assessment of immune activation via optical urine analysis. CyGbPF allows real-time non-invasive evaluation of cancer immunotherapeutic efficacy in living animals. CyGbPF can be used in research on cancers such as breast cancer. Excitation wavelength/emission wavelength: approximately 658 nm/approximately 717 nm^[1].

CyGbPF (2-250 μM; 5-90 min) is specifically cleaved by mouse granzyme B in cell-free assays, exhibiting a 24-fold near-infrared fluorescence enhancement^[1].
CyGbPF (5 μM; 0.5-2.0 h; 1.60-200 μg/mL) is rapidly activated by endogenous granzyme B in CD8⁺ T cells, with a time-dependent fluorescence enhancement amplitude approximately 35-fold higher than that in 4T1 cells and approximately 15-fold higher than that in RAW264.7 cells; moreover, it exhibits only extremely low cytotoxicity at concentrations up to 200 μg/mL across all three cell lines^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CyGbPF (10 μM/kg; intravenous injection; single administration) generates localized, activation-dependent NIRF signals in breast cancer mice, which correlate with the level of immune activation; non-invasive tracking of graded immune activation is achievable via tumor NIRF imaging and optical urine analysis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3367.84

C₁₆₃H₂₇₃IN₈O₅₆

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Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. He S, et al. Near-Infrared Fluorescent Macromolecular Reporters for Real-Time Imaging and Urinalysis of Cancer Immunotherapy. J Am Chem Soc. 2020 Apr 15;142(15):7075-7082.  [Content Brief]