[D-Phe2,D-Ala6]-LH-RH
[D-Phe2,D-Ala6]-LH-RH is a potent LH-RH antagonist. [D-Phe2,D-Ala6]-LH-RH shows anti-LH/FSH-RH and antiovulatory activities.
For research use only. We do not sell to patients.
- CAS No.: 54784-44-0
- Formula: C59H79N15O13
- Molecular Weight:1206.35
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
![[D-Phe2,D-Ala6]-LH-RH Chemical Structure](https://file.medchemexpress.com/product_pic/hy-p3664.gif "Click to View [D-Phe2,D-Ala6]-LH-RH Chemical Structure")
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
[D-Phe2,D-Ala6]-LH-RH (1 mg/rat, SC) shows marked antiovulatory and contraceptive activity[2]. [D-Phe2,D-Ala6]-LH-RH (0.625, 1.25, 2.5 mg/kg; s.c.; single dose; sacrificed 30 minutes) results a dose-dependent increase in stalk-median eminence (SME)-LHRH activity and decreases plasma LHRH activity as well in rats with hypophysectomized (hypox) at 25 days old[3].
[D-Phe2,D-Ala6]-LH-RH (25 mg/kg; s.c.; single dsoe) is effective in blocking the ovulation normally induced by LRH alone[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Adult female Sprague-Dawley rats (200-250 g)[2]
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Dosage:1 mg/rat
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Administration:SC injections, from 12:00 to 14:30 hr on the day of proestrus
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Result:Showed marked antiovulatory and contraceptive activity, with 96% inhibition of ovulation.
Chemical Information
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CAS No. 54784-44-0
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Molecular Weight 1206.35
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Formula C59H79N15O13
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Synonyms
Wy 18185
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Sequence Shortening
{Glp}-{d-Phe}-WSY-{d-Ala}-LRPG-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
| Contents | Assign value | |
|---|---|---|
| Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 |
| Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 |
| Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution:
| Overall charge of peptide | Details |
|---|---|
| Negative (<0) |
1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. |
| Positive (>0) |
1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. |
| Zero (=0) |
1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
Purity & Documentation
References
[1]. Vilchez-Martinez JA, et al. Comparison of the anti-LH/FSH-RH and anti-ovulatory activities of (D-Phe2, D-Leu6)-LH-RH and (D-Phe2, D-Ala6)-LH-RH. Endocr Res Commun. 1976;3(3-4):231-41. [Content Brief]
[2]. Beattie CW, et al. Luteinizing hormone-releasing hormone. Antiovulatory activity of analogs substituted in positions 2 and 6. J Med Chem. 1975 Dec;18(12):1247-50. [Content Brief]
[3]. Corbin A, et al. Effect of luteinizing hormone releasing hormone (LHRH) and an LHRH antagonist of hypothalamic and plasma LHRH of hypophysectomized rats. Endocrinology. 1976 Jan;98(1):247-50. [Content Brief]
[4]. Corbin A, et al. Ihibition of the pre-ovulatory proestrous gonadotropin surge, ovulation and pregnancy with a peptide analogue of luteinizing hormone releasing hormone. Endocr Res Commun. 1975;2(1):1-23. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
[D-Phe2,D-Ala6]-LH-RH Related Classifications
HY-P3664 Related Classifications
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