2. Apoptosis
  3. Apoptosis
  4. Eriocitrin

Eriocitrin  (Synonyms: Eriodictyol 7-rutinoside; Eriodictyol 7-O-rutinoside)

Cat. No.: HY-N0636 Purity: 99.84%
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SDS
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Eriocitrin is a flavonoid isolated from lemons that is a powerful antioxidant. Eriocitrin inhibits the proliferation of liver cancer cells by arresting the cell cycle in the S phase by upregulating p53, cyclin A, cyclin D3 and CDK6. Eriocitrin triggers apoptosis by activating intrinsic signaling pathways involving mitochondria.

For research use only. We do not sell to patients.

CAS No. : 13463-28-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Eriocitrin:

Eriocitrin Related Antibodies

Top Publications Citing Use of Products

    Eriocitrin purchased from MedChemExpress. Usage Cited in: BMC Cancer. 2025 Jan 13;25(1):76.  [Abstract]

    ATP assay analysis of the cell viability of HCT116 after Eriocitrin (6.25–600 μM; 24 h) treatment. Eriocitrin significantly inhibited the tumorigenic properties of CRC cells, as evidenced by a notable reduction in HCT116 cell proliferation.

    Eriocitrin purchased from MedChemExpress. Usage Cited in: BMC Cancer. 2025 Jan 13;25(1):76.  [Abstract]

    Colony formation assay analysis of the proliferation ability of HCT116 cells after Eriocitrin (50-200 μM; 24 h) treatment.

    Eriocitrin purchased from MedChemExpress. Usage Cited in: BMC Cancer. 2025 Jan 13;25(1):76.  [Abstract]

    Transwell assays analysis of the migration and invasion abilities of HCT116 cells after Eriocitrin (50-200 μM; 24 h) treatment. The results revealed that the invasion and migration abilities of HCT116 cells were attenuated by Eriocitrin intervention in a significant dose-dependent manner.

    Eriocitrin purchased from MedChemExpress. Usage Cited in: BMC Cancer. 2025 Jan 13;25(1):76.  [Abstract]

    Detection of CDK1 activity in HCT116 cells after Eriocitrin (50-200 μM; 24 h) treatment. The results showed that Eriocitrin inhibited the activity of CDK1 in a dose-dependent manner.

    Eriocitrin purchased from MedChemExpress. Usage Cited in: BMC Cancer. 2025 Jan 13;25(1):76.  [Abstract]

    Validation of the specific binding of Eriocitrin (0.00001-1 M; 24 h) to CDK1 by ITDR assay. The results illustrated that as the concentration of Eriocitrin increased, the thermal stability of CDK1 progressively enhanced.

    Eriocitrin purchased from MedChemExpress. Usage Cited in: BMC Cancer. 2025 Jan 13;25(1):76.  [Abstract]

    qRT-PCR analysis of the expression of CDK1 in HCT116 cells after Eriocitrin (50-200 μM; 24 h) treatment. The results showed that mRNA expression of CDK1 changed with the increase of Eriocitrin concentration.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Eriocitrin is a flavonoid isolated from lemons that is a powerful antioxidant. Eriocitrin inhibits the proliferation of liver cancer cells by arresting the cell cycle in the S phase by upregulating p53, cyclin A, cyclin D3 and CDK6. Eriocitrin triggers apoptosis by activating intrinsic signaling pathways involving mitochondria[1][1][2].

    In Vitro

    Eriocitrin (25-75 μM; 24 h) arrests the cell cycle in the S phase, (5-10 μM; 24 h) and inhibits proliferation and focus formation of liver cancer cell lines HepG2 and Huh7[1].
    Eriocitrin (25-75 μM; 12 h) upregulates p53, cyclin A, cyclin D3, and CDK6 levels in HepG2 cells[1].
    Eriocitrin (25-75 μM; 48 h) triggers apoptosis by activating mitochondria-related intrinsic signaling pathways[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Eriocitrin Related Antibodies
    In Vivo

    Eriocitrin (10-60 mg/kg; ip; once daily, from the day of modeling until the end of the experiment) attenuates acute kidney injury (AKI) induced by ischemia reperfusion (IR) in rats, and reduces renal tissue cell apoptosis, turns its kidney inflammatory response and oxidative stress[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    596.53

    Formula

    C27H32O15
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(C[C@@H](C1=CC(O)=C(O)C=C1)OC2=CC(O[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3CO[C@H](O[C@@H](C)[C@H](O)[C@H]4O)[C@@H]4O)=C5)C2=C5O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (167.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Methanol : 31.25 mg/mL (52.39 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6764 mL 8.3818 mL 16.7636 mL
    5 mM 0.3353 mL 1.6764 mL 3.3527 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (3.49 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (3.49 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.84%

    SDS (393 KB)

    COA (254 KB) HNMR (303 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Methanol / DMSO 1 mM 1.6764 mL 8.3818 mL 16.7636 mL 41.9090 mL
    5 mM 0.3353 mL 1.6764 mL 3.3527 mL 8.3818 mL
    10 mM 0.1676 mL 0.8382 mL 1.6764 mL 4.1909 mL
    15 mM 0.1118 mL 0.5588 mL 1.1176 mL 2.7939 mL
    20 mM 0.0838 mL 0.4191 mL 0.8382 mL 2.0955 mL
    25 mM 0.0671 mL 0.3353 mL 0.6705 mL 1.6764 mL
    30 mM 0.0559 mL 0.2794 mL 0.5588 mL 1.3970 mL
    40 mM 0.0419 mL 0.2095 mL 0.4191 mL 1.0477 mL
    50 mM 0.0335 mL 0.1676 mL 0.3353 mL 0.8382 mL
    DMSO 60 mM 0.0279 mL 0.1397 mL 0.2794 mL 0.6985 mL
    80 mM 0.0210 mL 0.1048 mL 0.2095 mL 0.5239 mL
    100 mM 0.0168 mL 0.0838 mL 0.1676 mL 0.4191 mL
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    Eriocitrin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Eriocitrin13463-28-0Eriodictyol 7-rutinoside Eriodictyol 7-O-rutinosideApoptosisCell cycle arrestHepatocellular carcinomaInhibitorinhibitorinhibit

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