Cat. No.: HY-N1445

Isoquercitrin

(Synonyms: Isoquercetin; Quercetin 3-glucoside)

8 Cited Publications

Customer Review

Based on 8 publication(s) in Google Scholar

Isoquercetin (Quercetin 3-glucoside) is a naturally occurring polyphenol that has antioxidant, anti-proliferative, and anti-inflammatory properties. Isoquercetin alleviates ethanol-induced hepatotoxicity, oxidative stress, and inflammatory responses via the Nrf2/ARE antioxidant signaling pathway. Isoquercetin regulates the expression of nitric oxide synthase 2 (NO2) via modulating the nuclear factor-κB (NF-κB) transcription regulation system. Isoquercetin has high bioavailability and low toxicity, is a promising candidate agent to prevent birth defects in diabetic pregnancies.

For research use only. We do not sell to patients.

Purity: 99.64%
CAS No.: 482-35-9
Formula: C₂₁H₂₀O₁₂
Molecular Weight:464.38

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Isoquercitrin Chemical Structure

CAS No.: 482-35-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Clinical Trial
Chemical Information
Publications (8)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Isoquercetin (Quercetin 3-glucoside) is a naturally occurring polyphenol that has antioxidant, anti-proliferative, and anti-inflammatory properties. Isoquercetin alleviates ethanol-induced hepatotoxicity, oxidative stress, and inflammatory responses via the Nrf2/ARE antioxidant signaling pathway^[1]. Isoquercetin regulates the expression of nitric oxide synthase 2 (NO2) via modulating the nuclear factor-κB (NF-κB) transcription regulation system. Isoquercetin has high bioavailability and low toxicity, is a promising candidate agent to prevent birth defects in diabetic pregnancies^[2].

IC₅₀ & Target

iNOS

All NF-κB Isoforms :

All NO Synthase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>10 μM Compound: 11	Cytotoxicity against human A549 cells after 48 hrs by SRB assay Cytotoxicity against human A549 cells after 48 hrs by SRB assay	[PMID: 28165740]
B16-4A5	IC₅₀	>100 μM Compound: 9	Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs by microplate reader analysis Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs by microplate reader analysis	[PMID: 25987378]
BMDC	IC₅₀	>50 μM Compound: 15	Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BMDC	IC₅₀	>50 μM Compound: 15	Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BMDC	IC₅₀	>50 μM Compound: 15	Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BT-549	IC₅₀	>10 μM Compound: 11	Cytotoxicity against human BT549 cells after 48 hrs by SRB assay Cytotoxicity against human BT549 cells after 48 hrs by SRB assay	[PMID: 28165740]
BV-2	IC₅₀	13.39 μM Compound: 5	Antineuroinflammatory activity in human BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction Antineuroinflammatory activity in human BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction	[PMID: 27623545]
BV-2	IC₅₀	19.8 μM Compound: 18	Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method	[PMID: 23149227]
BV-2	IC₅₀	55.59 μM Compound: 11	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay	[PMID: 28165740]
HEK293	IC₅₀	33.6 μM Compound: 15	Inhibition of TNF-alpha-induced NFkappaB activation in human HEK293 cells after 6 hrs by luciferase reporter gene assay Inhibition of TNF-alpha-induced NFkappaB activation in human HEK293 cells after 6 hrs by luciferase reporter gene assay	[PMID: 21261296]
Jurkat	IC₅₀	>100 μg/mL Compound: 10	Cytotoxicity against human Jurkat T cells after 36 hrs by MTT assay Cytotoxicity against human Jurkat T cells after 36 hrs by MTT assay	[PMID: 21546250]
KB	ED₅₀	>100 μg/mL Compound: NSC-407304	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 469554]
MDCK	CC₅₀	>400 μg/mL Compound: 5	Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect	[PMID: 15104496]
MDCK	CC₅₀	>431 μM Compound: Isoquercitrin	Cytotoxicity against MDCK cells Cytotoxicity against MDCK cells	[PMID: 25096296]
MDCK	CC₅₀	23.4 μg/mL Compound: 5	Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect	[PMID: 15104496]
MDCK	ED₅₀	1.2 μM Compound: 2, Isoquercetin	Antiviral activity against influenza A virus (A/swine/OH/511445/2007(H1N1)) Oh7 infected in MDCK cells assessed as inhibition of viral replication after 4 days by quantitative RT-PCR Antiviral activity against influenza A virus (A/swine/OH/511445/2007(H1N1)) Oh7 infected in MDCK cells assessed as inhibition of viral replication after 4 days by quantitative RT-PCR	[PMID: 22115591]
MDCK	IC₅₀	>500 μg/mL Compound: 5	Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect	[PMID: 15104496]
MDCK	IC₅₀	23.4 μg/mL Compound: 5	Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect	[PMID: 15104496]
MDCK	IC₅₀	23.4 μg/mL Compound: 5	Cytotoxicity against MDCK cells Cytotoxicity against MDCK cells	[PMID: 15104496]
MDCK	IC₅₀	5.9 μg/mL Compound: 5	Antiviral activity against RSV Long in MDCK cells assessed as inhibition of virus-induced cytopathic effect Antiviral activity against RSV Long in MDCK cells assessed as inhibition of virus-induced cytopathic effect	[PMID: 15104496]
N9	IC₅₀	75.2 μM Compound: 15	Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 28073678]
Raji	IC₅₀	570 molar ratio Compound: 14	Inhibition of TPA-induced EBV-early antigen activation in human Raji cells relative to TPA Inhibition of TPA-induced EBV-early antigen activation in human Raji cells relative to TPA	[PMID: 17190444]
RAW264.7	IC₅₀	>50 μM Compound: 17	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs by sandwich ELISA Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs by sandwich ELISA	[PMID: 24679441]
RAW264.7	IC₅₀	61.2 μM Compound: 10	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method	[PMID: 21353543]
RAW264.7	IC₅₀	76.3 μM Compound: 10	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs	[PMID: 21353543]
SK-MEL-2	IC₅₀	>10 μM Compound: 11	Cytotoxicity against human SK-MEL-2 cells after 48 hrs by SRB assay Cytotoxicity against human SK-MEL-2 cells after 48 hrs by SRB assay	[PMID: 28165740]
SK-OV-3	IC₅₀	>10 μM Compound: 11	Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay	[PMID: 28165740]

In Vitro

Isoquercetin (Quercetin 3-glucoside; 5-20 μM; 24 hours) substantially reduces ethanol-induced cytotoxicity , protects hepatic cells against ethanol-stimulated liver injury^[1]. Isoquercetin (10 μM; pre-treat 1 hour) dramatically downregulates the levels of ethanol-induced iNOS protein expression in HepG2 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

HepG2 cells
Concentration:

5 μM, 10 μM, 20 μM
Incubation Time:

24 hours
Result:

Caused significantly enhanced cell viability as positive controls.

Western Blot Analysis^[1]

Cell Line:

HepG2 cells
Concentration:

10 μM
Incubation Time:

1 hour
Result:

Decreased ethanol‐ induced iNOS protein expression.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 482-35-9
Appearance Solid
Molecular Weight 464.38
Formula C₂₁H₂₀O₁₂
Color Light yellow to yellow
SMILES

O=C1C(O[C@H]2[C@@H]([C@H]([C@@H]([C@@H](CO)O2)O)O)O)=C(C3=CC=C(O)C(O)=C3)OC4=CC(O)=CC(O)=C14
Synonyms

Isoquercetin; Quercetin 3-glucoside
Structure Classification
- Flavonoids
- Flavonols
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (8)

All Cell Imaging/StainingRT-PCRWBIn Vivo Imaging

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Journal Impact Factor

Nat Aging

Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice. [Abstract]

2024 Sep;4(9):1231-1248. PMID: 38951692

Isoquercitrin purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Sep;4(9):1231-1248. [Abstract]

Quantification of SA-β-Gal assay of UV-B-irradiated IMR90 fibroblasts, treated with the indicated compounds at 1 μM concentration Isoquercetin.
Sci Adv

In situ protein corona-camouflaged supramolecular assemblies remodel thrombotic microenvironment for improved arterial homeostasis. [Abstract]

2025 May 2;11(18):eadu6676. PMID: 40315315
Phytother Res

Isoquercitrin Promotes Angiogenesis Through the Genomic Signaling Pathway of the Estrogen Receptor-Alpha. [Abstract]

2025 Oct;39(10):4802-4820. PMID: 40908639

Isoquercitrin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Oct;39(10):4802-4820. [Abstract]

Fluorescence image of ERα nuclear translocation by 100 nM Isoquercitrin (IQ). 17β-estradiol (E2,10 nM) serves as a positive control.

Isoquercitrin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Oct;39(10):4802-4820. [Abstract]

The mRNA expression of IGFBP4 and PGR was measured using RT-qPCR in EAhy926 KRR cells treated with 100 nM Isoquercitrin (IQ) or 10 nM E2.

Isoquercitrin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Oct;39(10):4802-4820. [Abstract]

Isoquercitrin (IQ) (50 mg/kg, i.p.) suppresses the protein expression of p-NF-κB, p-c-JUN, and p-p38 by western blot.

Isoquercitrin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Oct;39(10):4802-4820. [Abstract]

The mice were treated with 100 μg/kg E2 or 50 mg/kg Isoquercitrin (IQ) (i.p.) every day, the expression level of VEGFR2 in VEGFR2-Luc female mice was monitored by bioluminescent imaging.
Cell Rep

Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation. [Abstract]

2023 Nov 10;42(11):113417. PMID: 37950872
Molecules

Roseoside Is a Bioactive Compound in Kirengeshoma koreana Nakai Extract with Potent In Vitro Antiviral Activity Against Hepatitis C Virus. [Abstract]

2024 Oct 30;29(21):5130. PMID: 39519772
J Sep Sci

Oriented Immobilization Coupled With Ligand Fishing Strategy for Rapid Discovery of Direct HMGB1 Inhibitors in Taxus wallichiana var. mairei. [Abstract]

2026 Apr;49(4):e70402. PMID: 41906428
Chem Biol Drug Des

Isoquercetin Ameliorates Osteoarthritis via Nrf2/NF-κB Axis: An In Vitro and In Vivo Study. [Abstract]

2024 Sep;104(3):e14620. PMID: 39251394
Eur Rev Med Pharmacol Sci

Mulberry leaf extracts and quercetin glycosides promote glycogen accumulation in astrocytes. [Abstract]

2025 Sep;29(9):425-433. PMID: 41059752

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (215.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1534 mL

10.7670 mL

21.5341 mL

5 mM

0.4307 mL

2.1534 mL

4.3068 mL

10 mM

0.2153 mL

1.0767 mL

2.1534 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 5 mg/mL (10.77 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 5 mg/mL (10.77 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lee S, et al. Relative protective activities of quercetin, quercetin-3-glucoside, and rutin in alcohol-induced liver injury. J Food Biochem. 2019 Aug 5:e13002. [Content Brief]

[2]. Tan C, et al. Modulation of nuclear factor-κB signaling and reduction of neural tube defects by quercetin-3-glucoside in embryos of diabetic mice. Am J Obstet Gynecol. 2018 Aug;219(2):197.e1-197.e8. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1534 mL	10.7670 mL	21.5341 mL	53.8352 mL
	5 mM	0.4307 mL	2.1534 mL	4.3068 mL	10.7670 mL
	10 mM	0.2153 mL	1.0767 mL	2.1534 mL	5.3835 mL
	15 mM	0.1436 mL	0.7178 mL	1.4356 mL	3.5890 mL
	20 mM	0.1077 mL	0.5384 mL	1.0767 mL	2.6918 mL
	25 mM	0.0861 mL	0.4307 mL	0.8614 mL	2.1534 mL
	30 mM	0.0718 mL	0.3589 mL	0.7178 mL	1.7945 mL
	40 mM	0.0538 mL	0.2692 mL	0.5384 mL	1.3459 mL
	50 mM	0.0431 mL	0.2153 mL	0.4307 mL	1.0767 mL
	60 mM	0.0359 mL	0.1795 mL	0.3589 mL	0.8973 mL
	80 mM	0.0269 mL	0.1346 mL	0.2692 mL	0.6729 mL
	100 mM	0.0215 mL	0.1077 mL	0.2153 mL	0.5384 mL

Keywords

Isoquercitrin

8 Cited Publications

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Isoquercitrin

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Publications Citing Use of MedChemExpress (MCE) Isoquercitrin

All NF-κB Isoforms

All NO Synthase Isoforms

Biological Activity

Chemical Information

Publications (8)

Solvent & Solubility