Isoquercitrin (Synonyms: Isoquercetin; Quercetin 3-glucoside)

Name: Isoquercitrin
Brand: MedChemExpress
SKU: HY-N1445
Rating: 5.0 (7 reviews)

Cat. No.: HY-N1445 Purity: 99.64%: Data Sheet
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Isoquercetin (Quercetin 3-glucoside) is a naturally occurring polyphenol that has antioxidant, anti-proliferative, and anti-inflammatory properties. Isoquercetin alleviates ethanol-induced hepatotoxicity, oxidative stress, and inflammatory responses via the Nrf2/ARE antioxidant signaling pathway. Isoquercetin regulates the expression of nitric oxide synthase 2 (NO2) via modulating the nuclear factor-κB (NF-κB) transcription regulation system. Isoquercetin has high bioavailability and low toxicity, is a promising candidate agent to prevent birth defects in diabetic pregnancies.

For research use only. We do not sell to patients.

CAS No. : 482-35-9

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10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Isoquercitrin:

Isoquercitrin (Standard) In-stock

7 Publications Citing Use of MCE Isoquercitrin

•Eur Rev Med Pharmacol Sci. 2025 Sep;29(9):425-433. [Abstract]

Cell Imaging/Staining

RT-PCR

In Vivo Imaging

: Isoquercitrin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Oct;39(10):4802-4820. [Abstract]; Fluorescence image of ERα nuclear translocation by 100 nM Isoquercitrin (IQ). 17β-estradiol (E2,10 nM) serves as a positive control.

: Isoquercitrin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Oct;39(10):4802-4820. [Abstract]; The mRNA expression of IGFBP4 and PGR was measured using RT-qPCR in EAhy926 KRR cells treated with 100 nM Isoquercitrin (IQ) or 10 nM E2.

: Isoquercitrin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Oct;39(10):4802-4820. [Abstract]; Isoquercitrin (IQ) (50 mg/kg, i.p.) suppresses the protein expression of p-NF-κB, p-c-JUN, and p-p38 by western blot.

: Isoquercitrin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Oct;39(10):4802-4820. [Abstract]; The mice were treated with 100 μg/kg E2 or 50 mg/kg Isoquercitrin (IQ) (i.p.) every day, the expression level of VEGFR2 in VEGFR2-Luc female mice was monitored by bioluminescent imaging.

: Isoquercitrin purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Sep;4(9):1231-1248.; Quantification of SA-β-Gal assay of UV-B-irradiated IMR90 fibroblasts, treated with the indicated compounds at 1 μM concentration Isoquercetin.

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nNOS iNOS eNOS

Biological Activity
Purity & Documentation
References
Customer Review

Description

Isoquercetin (Quercetin 3-glucoside) is a naturally occurring polyphenol that has antioxidant, anti-proliferative, and anti-inflammatory properties. Isoquercetin alleviates ethanol-induced hepatotoxicity, oxidative stress, and inflammatory responses via the Nrf2/ARE antioxidant signaling pathway^[1]. Isoquercetin regulates the expression of nitric oxide synthase 2 (NO2) via modulating the nuclear factor-κB (NF-κB) transcription regulation system. Isoquercetin has high bioavailability and low toxicity, is a promising candidate agent to prevent birth defects in diabetic pregnancies^[2].

IC₅₀ & Target

iNOS

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 10 μM Compound: 11	Cytotoxicity against human A549 cells after 48 hrs by SRB assay Cytotoxicity against human A549 cells after 48 hrs by SRB assay	[PMID: 28165740]
B16-4A5	IC₅₀	> 100 μM Compound: 9	Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs by microplate reader analysis Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs by microplate reader analysis	[PMID: 25987378]
BMDC	IC₅₀	> 50 μM Compound: 15	Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BMDC	IC₅₀	> 50 μM Compound: 15	Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BMDC	IC₅₀	> 50 μM Compound: 15	Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BT-549	IC₅₀	> 10 μM Compound: 11	Cytotoxicity against human BT549 cells after 48 hrs by SRB assay Cytotoxicity against human BT549 cells after 48 hrs by SRB assay	[PMID: 28165740]
BV-2	IC₅₀	13.39 μM Compound: 5	Antineuroinflammatory activity in human BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction Antineuroinflammatory activity in human BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction	[PMID: 27623545]
BV-2	IC₅₀	19.8 μM Compound: 18	Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method	[PMID: 23149227]
BV-2	IC₅₀	55.59 μM Compound: 11	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay	[PMID: 28165740]
HEK293	IC₅₀	33.6 μM Compound: 15	Inhibition of TNF-alpha-induced NFkappaB activation in human HEK293 cells after 6 hrs by luciferase reporter gene assay Inhibition of TNF-alpha-induced NFkappaB activation in human HEK293 cells after 6 hrs by luciferase reporter gene assay	[PMID: 21261296]
Jurkat	IC₅₀	> 100 μg/mL Compound: 10	Cytotoxicity against human Jurkat T cells after 36 hrs by MTT assay Cytotoxicity against human Jurkat T cells after 36 hrs by MTT assay	[PMID: 21546250]
KB	ED₅₀	> 100 μg/mL Compound: NSC-407304	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 469554]
MDCK	CC₅₀	23.4 μg/mL Compound: 5	Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect	[PMID: 15104496]
MDCK	CC₅₀	> 400 μg/mL Compound: 5	Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect	[PMID: 15104496]
MDCK	CC₅₀	> 431 μM Compound: Isoquercitrin	Cytotoxicity against MDCK cells Cytotoxicity against MDCK cells	[PMID: 25096296]
MDCK	ED₅₀	1.2 μM Compound: 2, Isoquercetin	Antiviral activity against influenza A virus (A/swine/OH/511445/2007(H1N1)) Oh7 infected in MDCK cells assessed as inhibition of viral replication after 4 days by quantitative RT-PCR Antiviral activity against influenza A virus (A/swine/OH/511445/2007(H1N1)) Oh7 infected in MDCK cells assessed as inhibition of viral replication after 4 days by quantitative RT-PCR	[PMID: 22115591]
MDCK	IC₅₀	23.4 μg/mL Compound: 5	Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect	[PMID: 15104496]
MDCK	IC₅₀	23.4 μg/mL Compound: 5	Cytotoxicity against MDCK cells Cytotoxicity against MDCK cells	[PMID: 15104496]
MDCK	IC₅₀	5.9 μg/mL Compound: 5	Antiviral activity against RSV Long in MDCK cells assessed as inhibition of virus-induced cytopathic effect Antiviral activity against RSV Long in MDCK cells assessed as inhibition of virus-induced cytopathic effect	[PMID: 15104496]
MDCK	IC₅₀	> 500 μg/mL Compound: 5	Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect	[PMID: 15104496]
N9	IC₅₀	75.2 μM Compound: 15	Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 28073678]
RAW264.7	IC₅₀	61.2 μM Compound: 10	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method	[PMID: 21353543]
RAW264.7	IC₅₀	76.3 μM Compound: 10	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs	[PMID: 21353543]
RAW264.7	IC₅₀	> 50 μM Compound: 17	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs by sandwich ELISA Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs by sandwich ELISA	[PMID: 24679441]
Raji	IC₅₀	570 molar ratio Compound: 14	Inhibition of TPA-induced EBV-early antigen activation in human Raji cells relative to TPA Inhibition of TPA-induced EBV-early antigen activation in human Raji cells relative to TPA	[PMID: 17190444]
SK-MEL-2	IC₅₀	> 10 μM Compound: 11	Cytotoxicity against human SK-MEL-2 cells after 48 hrs by SRB assay Cytotoxicity against human SK-MEL-2 cells after 48 hrs by SRB assay	[PMID: 28165740]
SK-OV-3	IC₅₀	> 10 μM Compound: 11	Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay	[PMID: 28165740]

In Vitro

Isoquercetin (Quercetin 3-glucoside; 5-20 μM; 24 hours) substantially reduces ethanol-induced cytotoxicity , protects hepatic cells against ethanol-stimulated liver injury^[1]. Isoquercetin (10 μM; pre-treat 1 hour) dramatically downregulates the levels of ethanol-induced iNOS protein expression in HepG2 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HepG2 cells
Concentration:	5 μM, 10 μM, 20 μM
Incubation Time:	24 hours
Result:	Caused significantly enhanced cell viability as positive controls.

Western Blot Analysis^[1]

Cell Line:	HepG2 cells
Concentration:	10 μM
Incubation Time:	1 hour
Result:	Decreased ethanol‐ induced iNOS protein expression.

Clinical Trial

Molecular Weight

464.38

Formula

C₂₁H₂₀O₁₂

CAS No.

482-35-9

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C(O[C@H]2[C@@H]([C@H]([C@@H]([C@@H](CO)O2)O)O)O)=C(C3=CC=C(O)C(O)=C3)OC4=CC(O)=CC(O)=C14

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (215.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1534 mL	10.7670 mL	21.5341 mL
5 mM	0.4307 mL	2.1534 mL	4.3068 mL
10 mM	0.2153 mL	1.0767 mL	2.1534 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (10.77 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (10.77 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.64%

Select Batch:

Data Sheet (281 KB) SDS (393 KB)

COA (257 KB) HNMR (80 KB) RP-HPLC (248 KB)

Handling Instructions (2659 KB)

References

[1]. Lee S, et al. Relative protective activities of quercetin, quercetin-3-glucoside, and rutin in alcohol-induced liver injury. J Food Biochem. 2019 Aug 5:e13002. [Content Brief]

[2]. Tan C, et al. Modulation of nuclear factor-κB signaling and reduction of neural tube defects by quercetin-3-glucoside in embryos of diabetic mice. Am J Obstet Gynecol. 2018 Aug;219(2):197.e1-197.e8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1534 mL	10.7670 mL	21.5341 mL	53.8352 mL
	5 mM	0.4307 mL	2.1534 mL	4.3068 mL	10.7670 mL
	10 mM	0.2153 mL	1.0767 mL	2.1534 mL	5.3835 mL
	15 mM	0.1436 mL	0.7178 mL	1.4356 mL	3.5890 mL
	20 mM	0.1077 mL	0.5384 mL	1.0767 mL	2.6918 mL
	25 mM	0.0861 mL	0.4307 mL	0.8614 mL	2.1534 mL
	30 mM	0.0718 mL	0.3589 mL	0.7178 mL	1.7945 mL
	40 mM	0.0538 mL	0.2692 mL	0.5384 mL	1.3459 mL
	50 mM	0.0431 mL	0.2153 mL	0.4307 mL	1.0767 mL
	60 mM	0.0359 mL	0.1795 mL	0.3589 mL	0.8973 mL
	80 mM	0.0269 mL	0.1346 mL	0.2692 mL	0.6729 mL
	100 mM	0.0215 mL	0.1077 mL	0.2153 mL	0.5384 mL

Isoquercitrin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Isoquercitrin482-35-9Isoquercetin Quercetin 3-glucosideNF-κBNO SynthaseNuclear factor-κBNuclear factor-kappaBNitric oxide synthasesNOSInhibitorinhibitorinhibit

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Isoquercitrin (Synonyms: Isoquercetin; Quercetin 3-glucoside)

Customer Review

Other Forms of Isoquercitrin:

Isoquercitrin Related Antibodies

7 Publications Citing Use of MCE Isoquercitrin

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Biological Activity

Purity & Documentation

References

Customer Review

Isoquercitrin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Isoquercitrin Related Classifications

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