Roseoside Is a Bioactive Compound in Kirengeshoma koreana Nakai Extract with Potent In Vitro Antiviral Activity Against Hepatitis C Virus

  • Molecules. 2024 Oct 30;29(21):5130. doi: 10.3390/molecules29215130.
Jun-Kyu Lee  1 ,  Ji-Wan Choi  1 ,  InWha Park  2 ,  Na-Eun Kim  1 ,  Hak Cheol Kwon  2 ,  Jaeyoung Kwon  2 ,  Yoon-Jae Song  1
Affiliations
  • 1. Department of Life Science, Gachon University, Seongnam 13120, Republic of Korea.
  • 2. Natural Product Informatics Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Gangneung 25451, Republic of Korea.
PMID: 39519772 DOI: 10.3390/molecules29215130
Abstract

Hepatitis C virus (HCV) is a pathogen that causes cirrhosis and hepatocellular carcinoma through chronic hepatitis C. This study focused on the anti-HCV activity of a 70% ethanol extract of Kirengeshoma koreana Nakai (KKE) and its bioactive chemical constituent(s). The KKE and its n-butanol (n-BuOH) fraction induced a significant reduction in HCV RNA levels without inducing cytotoxicity. A high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis revealed the presence of roseoside in the n-butanol fraction of the KKE, which inhibited HCV RNA replication in a concentration- and time-dependent manner without exerting cytotoxicity. Consistent with in silico molecular docking analysis data, roseoside targets and inhibits HCV NS5A/B replicase. Collectively, our findings demonstrate that roseoside is a chemical constituent in KKE that interferes with HCV replication by targeting NS5A/B replicase.

Keywords
Kirengeshoma koreana Nakai; antiviral; hepatitis C virus; roseoside.
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