Ethyl cinnamate

Name: Ethyl cinnamate
Brand: MedChemExpress
SKU: HY-Y0121
Rating: 4.5 (0 reviews)

Cat. No.: HY-Y0121 Purity: 99.80%: Data Sheet
COA

SDS
Handling Instructions
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Ethyl cinnamate, an orally active chemical constituent of the rhizome of Kaempferia galanga, exhibits anti-cancer, nematocidal, sedative and vasorelaxant activities. Ethyl cinnamate is a fragrance ingredient used as a food flavor and additive for cosmetic products. Ethyl cinnamate is also an excellent clearing reagent for mammalian tissues. Ethyl cinnamate suppresses tumor growth through anti-angiogenesis by attenuating VEGFR2 signal pathway in colorectal cancer. Ethyl cinnamate inhibits the tonic contractions induced by high K+ and phenylephrine (PE) with respective IC₅₀ values of 0.30 mM and 0.38 mM in rat aorta.

For research use only. We do not sell to patients.

Ethyl cinnamate Chemical Structure

CAS No. : 103-36-6

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
100 g	In-stock	Estimated Time of Arrival: December 31
250 g	In-stock	Estimated Time of Arrival: December 31
500 g	In-stock	Estimated Time of Arrival: December 31
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Other Forms of Ethyl cinnamate:

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VEGFR1/Flt-1 VEGFR2/KDR/Flk-1 VEGFR3/Flt-4

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	31.59 μM Compound: 7	Cytotoxicity against human A375 cells by MTT assay Cytotoxicity against human A375 cells by MTT assay	[PMID: 19782567]
A549	IC₅₀	50.35 μM Compound: 7	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 19782567]
HEp-2	ED₅₀	>50 μg/mL Compound: Ethyl cinnamate	Cytotoxicity against human Hep2 cells assessed a growth inhibition by rapid microtiter technique Cytotoxicity against human Hep2 cells assessed a growth inhibition by rapid microtiter technique	[PMID: 691008]
HL-60	IC₅₀	35.69 μM Compound: 7	Cytotoxicity against human HL60 cells by MTT assay Cytotoxicity against human HL60 cells by MTT assay	[PMID: 19782567]
HUVEC	IC₅₀	95 μg/mL Compound: 51	Antiinflammatory activity against TNF-alpha-induced ICAM1 protein expression in HUVEC pretreated for 2 hrs before TNFalpha challenge measured after 16 hrs by whole cell ELISA Antiinflammatory activity against TNF-alpha-induced ICAM1 protein expression in HUVEC pretreated for 2 hrs before TNFalpha challenge measured after 16 hrs by whole cell ELISA	[PMID: 21955679]
THP-1	IC₅₀	41.23 μM Compound: 7	Cytotoxicity against human THP1 cells by MTT assay Cytotoxicity against human THP1 cells by MTT assay	[PMID: 19782567]

In Vitro

Ethyl cinnamate (0-80 μM, 24 h and 48 h) inhibits VEGF-induced viability, motility, tube formation of Human umbilical vein endothelial cells (HUVECs) in a VEGFR2-dependent manner and induces HUVECs apoptosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[3]

Cell Line:	HUVECs
Concentration:	0-80 μM
Incubation Time:	24 h
Result:	Significantly inhibited HUVECs proliferation enhanced by vascular endothelial growth factor (VEGF).

Apoptosis Analysis^[3]

Cell Line:	HUVECs
Concentration:	0-80 μM
Incubation Time:	48 h
Result:	Increased apoptosis rate of cells and decreased the expression levels of Bcl-2/Bax with an IC₅₀ value of 31.79 μM in HUVECs.

In Vivo

Ethyl cinnamate (1-4 μM, incubation, a single dose) inhibits blood vessel formation in zebrafish embryos^[3].
Ethyl cinnamate (15, 30, 60 mg/kg, p.o., daily for 21 days) suppresses tumor growth and angiogenesis of HT29 nude mouse colon cancer xenograft model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Zebrafish embryos^[3]
Dosage:	1-4 μM
Administration:	incubation, a single dose for 24 h and 48 h
Result:	Significantly decreased the number of intact intersegmental vessels (ISVs) and inhibited the growth of SIVs in Zebrafish embryos.

Animal Model:	HT29 nude mouse colon cancer xenograft model^[3]
Dosage:	15, 30, 60 mg/kg
Administration:	p.o., daily for 21 days
Result:	Significantly inhibited the tumor growth and inhibited the expression levels of p-VEGFR2, p-Akt, p-ERK1/2, CD34, VEGF and Bcl-2/Bax in a dose-dependent manner in HT29 nude mouse colon cancer xenograft model.

Molecular Weight

176.22

Formula

C₁₁H₁₂O₂

CAS No.

103-36-6

Appearance

Liquid (Density: 1.049 g/cm³)

Color

Colorless to light yellow

SMILES

O=C(OCC)/C=C/C1=CC=CC=C1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (567.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (567.47 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.6747 mL	28.3736 mL	56.7472 mL
5 mM	1.1349 mL	5.6747 mL	11.3494 mL
10 mM	0.5675 mL	2.8374 mL	5.6747 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (14.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (276 KB) SDS (396 KB)

COA (244 KB) HNMR (130 KB) RP-HPLC (232 KB)

Handling Instructions (2659 KB)

References

[1]. S P Bhatia, et al. Fragrance material review on ethyl cinnamate. Food Chem Toxicol. 2007;45 Suppl 1:S90-4. [Content Brief]

[2]. Anika Klingberg, et al. Fully Automated Evaluation of Total Glomerular Number and Capillary Tuft Size in Nephritic Kidneys Using Lightsheet Microscopy. J Am Soc Nephrol. 2017 Feb;28(2):452-459. [Content Brief]

[3]. Wang S, et al. Ethyl cinnamate suppresses tumor growth through anti-angiogenesis by attenuating VEGFR2 signal pathway in colorectal cancer. J Ethnopharmacol. 2024 May 23;326:117913. [Content Brief]

[4]. Othman R, et al. Vasorelaxant effects of ethyl cinnamate isolated from Kaempferia galanga on smooth muscles of the rat aorta. Planta Med. 2002 Jul;68(7):655-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	5.6747 mL	28.3736 mL	56.7472 mL	141.8681 mL
	5 mM	1.1349 mL	5.6747 mL	11.3494 mL	28.3736 mL
	10 mM	0.5675 mL	2.8374 mL	5.6747 mL	14.1868 mL
	15 mM	0.3783 mL	1.8916 mL	3.7831 mL	9.4579 mL
	20 mM	0.2837 mL	1.4187 mL	2.8374 mL	7.0934 mL
	25 mM	0.2270 mL	1.1349 mL	2.2699 mL	5.6747 mL
	30 mM	0.1892 mL	0.9458 mL	1.8916 mL	4.7289 mL
	40 mM	0.1419 mL	0.7093 mL	1.4187 mL	3.5467 mL
	50 mM	0.1135 mL	0.5675 mL	1.1349 mL	2.8374 mL
	60 mM	0.0946 mL	0.4729 mL	0.9458 mL	2.3645 mL
	80 mM	0.0709 mL	0.3547 mL	0.7093 mL	1.7734 mL
	100 mM	0.0567 mL	0.2837 mL	0.5675 mL	1.4187 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Ethyl cinnamate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Ethyl cinnamate

Customer Review

Other Forms of Ethyl cinnamate:

Ethyl cinnamate Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

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View All Parasite Isoform Specific Products:

View All VEGFR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Ethyl cinnamate Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Ethyl cinnamate Related Classifications

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