Methscopolamine bromide
Based on 1 publication(s) in Google Scholar
Methscopolamine bromide ((-)-Scopolamine methyl bromide; Hyoscine methyl bromide) is a peripherally acting muscarinic receptor (mAChR) antagonist that cannot cross the blood-brain barrier. Methscopolamine bromide blocks the muscarinic negative feedback regulation of acetylcholine release from striatal cholinergic terminals, thereby increasing acetylcholine release in the striatum of freely moving rats. Methscopolamine bromide does not induce motor excitation in freely moving rats, nor does it alter the duration of ethanol-induced loss of righting reflex in mice. Methscopolamine bromide fails to antagonize the arecoline-mediated reduction in the duration of ethanol-induced loss of righting reflex in mice.
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- 純度: 99.53%
- CAS 番号: 155-41-9
- 分子式: C18H24BrNO4
- 分子量:398.29
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保管条件:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 Methscopolamine bromide
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生物活性
Methscopolamine bromide (5-10 mg/kg; i.p.; single administration) maximally increases acetylcholine release in the striatum of freely moving rats by 380% and 430%, respectively, without altering their spontaneous motor activity[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:ICR mice (male, 18 to 22 g)[1]
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Dosage:0.25 mg/kg; 1.0 mg/kg
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Administration:s.c.; single dose
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Result:Did not significantly alter the duration of ethanol-induced loss of the righting reflex (LORR) compared to saline-treated controls when administered alone.
Did not reverse the arecoline-induced shortening of ethanol-induced LORR duration (no significant difference compared to the saline + arecoline group) when administered before arecoline.
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Animal Model:Wistar rats (male, 280-350 g)[2]
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Dosage:5 mg/kg; 10 mg/kg
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Administration:i.p.; single injection
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Result:Caused a maximal 380% increase in striatal acetylcholine release relative to baseline levels at 2 hours post-injection, with release remaining above baseline for over 4 hours.
Caused a maximal 430% increase in striatal acetylcholine release relative to baseline levels at 2 hours post-injection, with release remaining above baseline for over 4 hours.
Did not cause a change in spontaneous motor activity relative to controls.
化学情報
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CAS 番号 155-41-9
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性状 Solid
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分子量 398.29
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分子式 C18H24BrNO4
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Color White to off-white
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SMILES
C[N+]1([C@@H]2[C@H](O3)[C@H]3[C@H]1C[C@@H](OC([C@@H](C4=CC=CC=C4)CO)=O)C2)C.[Br-]
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別名
(-)-Scopolamine methyl bromide; Hyoscine methyl bromide
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Oncol Lett
Effects of pharmacological treatments on hippocampal NCAM1 and ERK2 expression in epileptic rats with cognitive dysfunction. [Abstract]2016 Sep;12(3):1783-1791. PMID: 27588125
溶剤 & 溶解度
DMSO : 50 mg/mL (125.54 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (125.54 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
純度とドキュメンテーション
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データシート (279 KB)
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SDS (537 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Sun YP, et al. Systemic administration of arecoline reduces ethanol-induced sleeping through activation of central muscarinic receptor in mice. Alcohol Clin Exp Res. 2010;34(1):150-157. [Content Brief]
[2]. Watanabe H, et al. Effect of anticholinergic drugs on striatal acetylcholine release and motor activity in freely moving rats studied by brain microdialysis. Jpn J Pharmacol. 1989;51(1):75-82. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.5107 mL | 12.5537 mL | 25.1073 mL | 62.7683 mL |
| 5 mM | 0.5021 mL | 2.5107 mL | 5.0215 mL | 12.5537 mL | |
| 10 mM | 0.2511 mL | 1.2554 mL | 2.5107 mL | 6.2768 mL | |
| 15 mM | 0.1674 mL | 0.8369 mL | 1.6738 mL | 4.1846 mL | |
| 20 mM | 0.1255 mL | 0.6277 mL | 1.2554 mL | 3.1384 mL | |
| 25 mM | 0.1004 mL | 0.5021 mL | 1.0043 mL | 2.5107 mL | |
| 30 mM | 0.0837 mL | 0.4185 mL | 0.8369 mL | 2.0923 mL | |
| 40 mM | 0.0628 mL | 0.3138 mL | 0.6277 mL | 1.5692 mL | |
| 50 mM | 0.0502 mL | 0.2511 mL | 0.5021 mL | 1.2554 mL | |
| 60 mM | 0.0418 mL | 0.2092 mL | 0.4185 mL | 1.0461 mL | |
| 80 mM | 0.0314 mL | 0.1569 mL | 0.3138 mL | 0.7846 mL | |
| 100 mM | 0.0251 mL | 0.1255 mL | 0.2511 mL | 0.6277 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Methscopolamine bromide 関連分類
HY-B0344 関連分類
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