HDAC8-IN-15
HDAC8-IN-15 is a selective HDAC8 inhibitor with an IC50 of 0.40 μM. HDAC8-IN-15 increases the acetylation level of the HDAC8 substrate SMC3 without altering the total protein level of SMC3. HDAC8-IN-15 reduces cancer cell viability, inhibits colony formation, slows cell migration, induces apoptosis, and causes cell cycle arrest at the SubG1 phase. HDAC8-IN-15 can be used in studies related to neuroblastoma.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- 화학식: C26H27Cl3N6O
- 분자량:545.89
-
보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- 순도&문서
- References
-
Help & FAQs
Biological Activity
|
HDAC8 0.4 μM (IC50) |
HDAC8-IN-15 (Compound 9m) exerts a stronger proliferation-inhibitory effect on IMR32 neuroblastoma cells (IC50 = 9.92 μM) than on HCT-116 colon cancer cells, MCF7 breast cancer cells, and normal HEK293 cells[1].
HDAC8-IN-15 (1-5 μM; 9-12 days) potently inhibits colony formation of IMR32 neuroblastoma cells[1].
HDAC8-IN-15 (1-5 μM; 0-48 h) inhibits the migration of IMR32 neuroblastoma cells, and this effect is verified by reduced wound healing[1].
HDAC8-IN-15 (1-5 μM; 48 h) dose-dependently induces apoptosis in IMR32 neuroblastoma cells[1].
HDAC8-IN-15 (1-5 μM; 48 h) induces G1 phase cell cycle arrest in IMR32 neuroblastoma cells[1].
HDAC8-IN-15 (1-5 μM; 24 h) increases the acetylation level of SMC3 in IMR32 neuroblastoma cells, which is consistent with the inhibitory effect of HDAC8[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:IMR32 neuroblastoma cells
-
Concentration:1 μM, 5 μM
-
Incubation Time:0, 12 and 48 h
-
Result:Significantly reduced the percentage of wound closure over 48 h compared to untreated controls, indicating inhibited cell migration.
-
Cell Line:IMR32 neuroblastoma cells
-
Concentration:1 μM, 5 μM
-
Incubation Time:48 h
-
Result:Induced early apoptosis in ~5% of IMR32 cells and late apoptosis/necrosis in ~2% of cells at 1 μM.
Induced early apoptosis in ~11% of cells and late apoptosis/necrosis in ~5% of cells at 5 μM, compared to minimal apoptosis in untreated controls.
-
Cell Line:IMR32 neuroblastoma cells
-
Concentration:1 μM, 5 μM
-
Incubation Time:48 h
-
Result:Increased the sub-G1 cell population to ~11.2% (from ~2.62% in controls) at 1 μM.
Increased the sub-G1 population to ~20.2% at 5 μM, indicating induction of cell cycle arrest at the sub-G1 phase.
-
Cell Line:IMR32 neuroblastoma cells
-
Concentration:1 μM, 5 μM
-
Incubation Time:24 h
-
Result:Significantly increased Ac-SMC3 protein levels relative to untreated controls, while total SMC3 and β-actin levels remained unchanged.
Chemical Information
-
분자량 545.89
-
화학식 C26H27Cl3N6O
-
SMILES
O=C(N1CCN(C2=CC3=C(N4C(C=C3)=NN=C4C5CC5)C=C2)CC1)[C@H](N)CC6=CC=C(Cl)C=C6Cl.Cl
-
선적
Room temperature in continental US; may vary elsewhere.
-
보관
Please store the product under the recommended conditions in the Certificate of Analysis.
순도&문서
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
HDAC8-IN-15 Related Classifications
HY-181979 Related Classifications
Powered by Bioz