1. Apoptosis
    Immunology/Inflammation
    Metabolic Enzyme/Protease
    NF-κB
  2. TNF Receptor
    Interleukin Related
    Toll-like Receptor (TLR)
    Reactive Oxygen Species
  3. NecroX-7

NecroX-7 

Cat. No.: HY-124750
Handling Instructions

NecroX-7 is a potent free radical scavenger and a HMGB1 (high-mobility group box 1) inhibitor. NecroX-7 can be used as an antidote to acetaminophen toxicity. NecroX-7 exerts a protective effect by preventing the release of HMGB1 in ischemia/reperfusion injury. NecroX-7 inhibits the HMGB1-induced release of TNF and IL-6, as well as the expression of TLR-4 and receptor for advanced glycation end products. NecroX-7 can be used graft-versus-host disease (GVHD) research.

For research use only. We do not sell to patients.

NecroX-7 Chemical Structure

NecroX-7 Chemical Structure

CAS No. : 1120332-55-9

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Description

NecroX-7 is a potent free radical scavenger and a HMGB1 (high-mobility group box 1) inhibitor. NecroX-7 can be used as an antidote to acetaminophen toxicity. NecroX-7 exerts a protective effect by preventing the release of HMGB1 in ischemia/reperfusion injury. NecroX-7 inhibits the HMGB1-induced release of TNF and IL-6, as well as the expression of TLR-4 and receptor for advanced glycation end products. NecroX-7 can be used graft-versus-host disease (GVHD) research[1].

IC50 & Target

IL-6

 

TLR4

 

In Vitro

NecroX-7 (0-40 μM, 3-4 d) suppresses activated or proliferating T cells without causing apoptosis[1].
NecroX-7 (0-40 μM) markedly reduces HMGB1 levels in a dose-dependent manner[1].
NecroX-7 inhibits formation of mitochondria-specific ROS/reactive nitrogen species in H9C2 cells and hepatocytes after induction by tert-butyl hydroperoxide or doxorubicin[1].
NecroX-7 increased regulatory T cell numbers, which may be associated with regulation of differentiation signals independent of HMGB1[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: CD4 T cells
Concentration: 0, 0.625, 1.25, 2.5, 5, 10, 20, and 40 μM
Incubation Time: 3-4 d
Result: Showed a marked reduction in splenocyte proliferation, in a dose-dependent manner. Modulated alloreactive T cell responses.
In Vivo

NecroX-7 (0-0.3 mg/kg, IV, once injection at 2-d intervals, for 2 weeks) significantly attenuates GVHD-related mortality and inhibits severe tissue damage[1].
NecroX-7 protects mice against lethal GVHD by reciprocal regulation of regulatory T/Th1 cells, attenuating systemic HMGB1 accumulation and inhibiting HMGB1-mediated inflammatory response[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female BALB/c and C57BL/6 mice (Eight-week-old, with GVHD)[1]
Dosage: 0.03, 0.1, and 0.3 mg/kg
Administration: IV, once injection at 2-d intervals, for 2 weeks
Result: Observed statistically significant prolonged survival at doses ≥0.1 mg/kg: 30–60% of mice in these treatment groups survived for >50 d. Significantly improved clinical signs and prolonged survival, and the mice showed a reduction in clinical manifestations of acute GVHD, including weight loss, hunched posture, diarrhea, and ruffled fur.
Molecular Weight

439.57

Formula

C24H29N3O3S

CAS No.
SMILES

O=S1(CCN(CC1)CC2=CC3=C(C(NC4CCOCC4)=C2)NC(C5=CC=CC=C5)=C3)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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NecroX-7
Cat. No.:
HY-124750
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