Cat. No.: HY-N1377

Nevadensin

(Synonyms: Lysionotin)

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Nevadensin (Lysionotin), a natural flavonoid, is a selective human carboxylesterase 1 (hCE1) inhibitor with an IC₅₀ of 2.64 μM. Nevadensin is more selective for hCE1 than hCE2 (IC₅₀ of 132.8 μM). Nevadensin can induce apoptosis and DNA damage in cancer cells. Nevadensin has a variety of pharmacological effects such as anti-inflammatory, anti-tumour, anti-hypertensive, anti-tubercular, antitussive, antioxidant and anti-microbial activities.

For research use only. We do not sell to patients.

Purity: 99.93%
CAS No.: 10176-66-6
Formula: C₁₈H₁₆O₇
Molecular Weight:344.32

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Nevadensin Chemical Structure

CAS No.: 10176-66-6

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

IC50: 2.64 μM (hCE1), 132.8 μM (hCE2)^[1]

All Carboxylesterase (CES) Isoforms :

All DNA/RNA Synthesis Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	5 μM Compound: 61; Lys	Antiproliferative activity against human HCT-116 cells Antiproliferative activity against human HCT-116 cells	[PMID: 38142509]
RAW264.7	IC₅₀	5.08 μM Compound: 6	Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells pre-incubated for 2 hrs before LPS stimulation for 24 hrs by Griess assay method Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells pre-incubated for 2 hrs before LPS stimulation for 24 hrs by Griess assay method	[PMID: 25176187]
SW480	IC₅₀	5 μM Compound: 61; Lys	Antiproliferative activity against human SW480 cells Antiproliferative activity against human SW480 cells	[PMID: 38142509]

In Vitro

Nevadensin exhibits inhibition activity against Mycobacterium tuberculosis, with a MIC value of 200 μg/mL. Nevadensin exhibits antioxidant activity against the radical scavenging ability of DPPH, with the IC₅₀ value of 7.7 μg/mL^[2].
Nevadensin potently inhibits p40 protein tyrosine kinase activity with an IC₅₀ value of 50 μg/mL^[3].
Nevadensin shows DNA-intercalating properties, with IC₅₀s of 31.63 µM and 296.91 µM for minor groove binding and DNA intercalation^[3].
Nevadensin (500 μM; 1 h) in human colon carcinoma cell line HT29 cells, significantly increases the amount of topoisomerase I (TOPO I) bound to DNA, but has no stabilizing effect on the TOPO IIα/DNA complex^[3].
Nevadensin (1-500 μM; 24-48 h) in HT29 cells, causes a slight but significant reduction in cell viability of about 20% at concentrations ≥250 μM after 24 h, and a significant decrease in cell viability but still higher than 50% after 48 h^[3].
Nevadensin (1-500 μM; 24 h) in HT29 cells, increases the number of cells in the G2/M phase at concentrations ≥100 μM^[3].
Nevadensin (50-500 μM; 24 h) in HT29 cells, increases the activities of caspase-3 and caspase-9 in a concentration-dependent manner and has no effect on the activity of caspase-8^[3].
Nevadensin (12.5-50 µM; for 24 h) significantly inhibits growth of hepatocellular carcinoma (HCC) cells via inducing cell cycle arrest and apoptosis. Nevadensin regulates multiple functional signaling pathways associated with cancer including Hippo signaling. Nevadensin notably induces activation of the MST1/2-LATS1/2 kinase in HCC cells^[4].
Nevadensin (1-10 μg/mL) inhibits the release of β-hexosaminidase and histamine in bone marrow-derived mast cells^[5].
Nevadensin (2-20 μg/mL; 3 days) in BMMCs (bone marrow-derived mast cells) inhibits cell proliferation, decreases the expression of c-Kit and accelerates their apoptosis^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

HT29 cells
Concentration:

1 μM, 10 μM, 100 μM, 250 μM, 500 μM
Incubation Time:

24 h, 48 h
Result:

After 24 h, at concentrations ≥250 μM, cell viability was slightly but significantly reduced by about 20%.

Cell Cycle Analysis^[3]

Cell Line:

HT29 cells
Concentration:

1 μM, 10 μM, 100 μM, 250 μM, 500 μM
Incubation Time:

24 h
Result:

At concentrations ≥100 μM, the number of cells in the G2/M phase increased.

Western Blot Analysis^[3]

Cell Line:

HT29 cells
Concentration:

50 μM, 100 μM, 250 μM, 500 μM
Incubation Time:

24 h
Result:

Increased the levels of apoptosis-related proteins such as Cleaved Caspase 3, Cleaved-PARP, and Cleaved Caspase 9 in a concentration-dependent manner.

In Vivo

Nevadensin (1-10 mg/kg; gavage; once a day; from day 27 to day 40) in ovalbumin (OVA)-induced mouse food allergy model alleviates allergic symptoms and decreases the levels of serum specific immunoglobulin E, histamine, and mouse mast cell protease-1^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female BALB/c mice (6-8 weeks) were sensitized through intraperitoneal injection of 100 μg of ovalbumin and 2 mg of alum in 200 μL of PBS on days 0 and 14, and then orally challenged with 50 mg of ovalbumin in 200 μL of PBS every 3 days from day 28 for a total of five challenge events^[5].
Dosage:

1, 5 or 10 mg/kg
Administration:

Oral gavage, once a day, from day 27 to day 40
Result:

Significantly attenuated oral ovalbumin-challenge-induced food allergy, as assessed by improvements in hypothermia, diarrhea, and anaphylaxis score.
Also led to significantly decreased levels of OVA-specific IgE, histamine, and mMCP-1 in the serum.

Chemical Information

CAS No. 10176-66-6
Appearance Solid
Molecular Weight 344.32
Formula C₁₈H₁₆O₇
Color Light yellow to yellow
SMILES

O=C1C=C(C2=CC=C(OC)C=C2)OC3=C(OC)C(O)=C(OC)C(O)=C13
Synonyms

Lysionotin
Structure Classification
- Flavonoids
- Flavones
- Phenols
- Polyphenols
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 14.71 mg/mL (42.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9043 mL

14.5214 mL

29.0428 mL

5 mM

0.5809 mL

2.9043 mL

5.8085 mL

10 mM

0.2904 mL

1.4521 mL

2.9043 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water

Solubility: 2.5 mg/mL (7.26 mM); Suspended solution; Need ultrasonic

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ya-Qiao Wang, et al. Nevadensin is a naturally occurring selective inhibitor of human carboxylesterase 1. Int J Biol Macromol. 2018 Dec;120(Pt B):1944-1954. [Content Brief]

[2]. Suksamrarn A, et al. Antimycobacterial and antioxidant flavones from Limnophila geoffrayi. Arch Pharm Res. 2003 Oct;26(10):816-20. [Content Brief]

[3]. Müller L, et al. Topoisomerase poisoning by the flavonoid nevadensin triggers DNA damage and apoptosis in human colon carcinoma HT29 cells. Arch Toxicol. 2021 Dec;95(12):3787-3802. [Content Brief]

[4]. Shi H, et al. Activating the Hippo pathway by nevadensin overcomes Yap-drived resistance to sorafenib in hepatocellular carcinoma. Discov Oncol. 2023 May 27;14(1):83. [Content Brief]

[5]. Zhang YF , et al. Nevadensin relieves food allergic responses and passive cutaneous anaphylaxis in mice through inhibiting the expression of c-Kit receptors. Food Funct. 2020 Dec 1;11(12):10375-10385. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9043 mL	14.5214 mL	29.0428 mL	72.6069 mL
	5 mM	0.5809 mL	2.9043 mL	5.8086 mL	14.5214 mL
	10 mM	0.2904 mL	1.4521 mL	2.9043 mL	7.2607 mL
	15 mM	0.1936 mL	0.9681 mL	1.9362 mL	4.8405 mL
	20 mM	0.1452 mL	0.7261 mL	1.4521 mL	3.6303 mL
	25 mM	0.1162 mL	0.5809 mL	1.1617 mL	2.9043 mL
	30 mM	0.0968 mL	0.4840 mL	0.9681 mL	2.4202 mL
	40 mM	0.0726 mL	0.3630 mL	0.7261 mL	1.8152 mL

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Nevadensin

Anti-Infection Compound Library

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

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Mongolian Medicine Compound Library

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Mass Spectrometry Natural Product Library

All Carboxylesterase (CES) Isoforms

All DNA/RNA Synthesis Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Nevadensin Related Classifications

HY-N1377 Related Classifications