Phosphoenolpyruvate carboxylase, Microorganism
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Phosphoenolpyruvate carboxylase, Microorganism (PEPC) is a carbon dioxide fixing enzyme that in an irreversible manner and in the presence of Mg2+, converts phosphoenolpyruvate and bicarbonate into oxaloacetate and inorganic phosphorus. Phosphoenolpyruvate carboxylase catalyses the primary assimilation of CO(2) in Crassulacean acid metabolism plants. Phosphoenolpyruvate carboxylase plays a major role in setting the day-night pattern of metabolism in plants.
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- CAS No.: 9067-77-0
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Product Information
Phosphoenolpyruvate carboxylase (PEPC) catalyzes the fixation of carbon dioxide to phosphoenolpyruvate to produce oxaloacetate and inorganic phosphate.
The enzyme is a tetramer composed of identical subunits with a molecular weight of approximately 100,000. There are 36 cysteine residues per mole in the form of free SH groups.
Optimum pH: 8.0-9.0, pH 8.5 is generally used
Activators: Acetyl CoA and Fructose-1,6-Bisphosphate
Inhibitors: Fumaric acid and L-Malic acid
Instructions
It is recommended to dissolve in 0.11 M Tris sulfate buffer (pH 8.5) containing 0.005 M magnesium sulfate.
Literature reports that PEPC solutions are stable for at least six hours in Mg2+ buffer. In dilute solutions, the enzyme dissociates into monomers and dimers and becomes inactive unless Mg2+ or aspartate is present.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
4.1.1.31
≥5 U/mg soild
≥80 U/mg protein
One unit is defined as the amount of enzyme that will convert 1μmol of phosphoenolpyruvate to oxaloacetate per min at pH 8.0 at 30°C.
Chemical Information
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CAS No. 9067-77-0
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Appearance Solid
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Color White to off-white
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SMILES
[Phosphoenolpyruvate carboxylase]
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Synonyms
PEPC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (265 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Durall C, et, al. Oligomerization and characteristics of phosphoenolpyruvate carboxylase in Synechococcus PCC 7002. Sci Rep. 2020 Feb 27;10(1):3607. [Content Brief]
[2]. Nimmo HG. The regulation of phosphoenolpyruvate carboxylase in CAM plants. Trends Plant Sci. 2000 Feb;5(2):75-80. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Phosphoenolpyruvate carboxylase, Microorganism Related Classifications
HY-E70015 Related Classifications
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