Cat. No.: HY-N6939

Pseudolaric Acid B

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Pseudolaric Acid B is an orally active diterpene acid. Pseudolaric Acid B has anti-fungal, anti-fertility, anti-angiogenesis and anticancer activity, and can induce tumor cell apoptosis and autophagy. In addition, Pseudolaric Acid B can inhibit the secretion of hepatitis B virus (HBV) and has immunosuppressive effect, selectively inhibiting the proliferation of T lymphocytes and the production of IL-2.

For research use only. We do not sell to patients.

Purity: 98.67%
CAS No.: 82508-31-4
Formula: C₂₃H₂₈O₈
Molecular Weight:432.46

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Pseudolaric Acid B Chemical Structure

CAS No.: 82508-31-4

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[3]

HIF-1α

All Interleukin Related Isoforms :

All HIF/HIF Prolyl-Hydroxylase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.3 μM Compound: PAB	Cytotoxicity against human A549 cells by SRB method Cytotoxicity against human A549 cells by SRB method	[PMID: 17253850]
A549	IC₅₀	0.3 μM Compound: pseudolaric acid B	Cytotoxicity against human A549 cells by SRB method Cytotoxicity against human A549 cells by SRB method	[PMID: 17323995]
A549	IC₅₀	0.3 μM Compound: pseudolaricacid B	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 18651771]
A549	IC₅₀	0.86 μg/mL Compound: PAB	Cytotoxicity against human A549 cells after 24 hrs Cytotoxicity against human A549 cells after 24 hrs	[PMID: 15387644]
A549	IC₅₀	1.6 μM Compound: Pseudolaric acid B	Cytotoxicity against human A549 cells after 72 hrs by SRB assay Cytotoxicity against human A549 cells after 72 hrs by SRB assay	[PMID: 20038159]
Bel-7402	IC₅₀	>100 μM Compound: 7	Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay	[PMID: 17291040]
Bel-7402	IC₅₀	0.704 μM Compound: 1, pseudolaric acid B, PAB	Antiproliferative activity against human BEL-7402 cells by SRB assay Antiproliferative activity against human BEL-7402 cells by SRB assay	[PMID: 18078313]
Bel-7402	IC₅₀	1.3 μM Compound: Pseudolaric acid B	Cytotoxicity against human Bel7402 cells after 72 hrs by SRB assay Cytotoxicity against human Bel7402 cells after 72 hrs by SRB assay	[PMID: 20038159]
BGC-823	IC₅₀	>100 μM Compound: 7	Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay	[PMID: 17291040]
HCT-116	IC₅₀	0.136 μM Compound: 1, pseudolaric acid B, PAB	Antiproliferative activity against human HCT116 cells by SRB assay Antiproliferative activity against human HCT116 cells by SRB assay	[PMID: 18078313]
HeLa	IC₅₀	0.497 μM Compound: 1, pseudolaric acid B, PAB	Antiproliferative activity against human Hela cells by SRB assay Antiproliferative activity against human Hela cells by SRB assay	[PMID: 18078313]
HL-60	IC₅₀	0.235 μM Compound: 1, pseudolaric acid B, PAB	Antiproliferative activity against human HL60 cells by SRB assay Antiproliferative activity against human HL60 cells by SRB assay	[PMID: 18078313]
HL-60	IC₅₀	0.59 μM Compound: 7	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 17291040]
HL-60	IC₅₀	4.2 μM Compound: Pseudolaric acid B	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 20038159]
HMEC-1	IC₅₀	0.803 μM Compound: 1, pseudolaric acid B, PAB	Antiproliferative activity against HMEC1 cells by SRB assay Antiproliferative activity against HMEC1 cells by SRB assay	[PMID: 18078313]
MDA-MB-468	IC₅₀	0.596 μM Compound: 1, pseudolaric acid B, PAB	Antiproliferative activity against human MB-MDA-468 cells by SRB assay Antiproliferative activity against human MB-MDA-468 cells by SRB assay	[PMID: 18078313]
P388	IC₅₀	0.32 μg/mL Compound: PAB	Cytotoxicity against mouse P388 cells after 24 hrs Cytotoxicity against mouse P388 cells after 24 hrs	[PMID: 15387644]
P388	IC₅₀	0.74 μM Compound: pseudolaric acid B	Cytotoxicity against mouse P388 cells by MTT method Cytotoxicity against mouse P388 cells by MTT method	[PMID: 17323995]
P388	IC₅₀	3.7 μM Compound: PAB	Cytotoxicity against murine P388 cells by MTT method Cytotoxicity against murine P388 cells by MTT method	[PMID: 17253850]

In Vitro

Pseudolaric Acid B (4 μM; 36 h to 3 days) activates autophagy in MCF‑7 human breast cancer cells to prevent cell death^[1].
Pseudolaric Acid B (4 μM; 0-36 h) inhibits the secretion of hepatitis B virus (HBV) by inducing apoptosis and cell cycle arrest^[2].
Pseudolaric Acid B (0.313-10 μM; 6-72 h) inhibits the proliferation, migration and tube formation of HUVEC, and reduces the secretion of VEGF by tumor cells, which is related to proteasoma-mediated HIF-1α protein degradation^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

MCF-7 cells
Concentration:

4 μM
Incubation Time:

36 h
Result:

Increased the expression of Beclin-1.
Promoted the conversion from LC3-I to LC3-II.

Immunofluorescence^[1]

Cell Line:

MCF-7 cells
Concentration:

4 μM
Incubation Time:

36 h
Result:

Increased the number of autophagic vacuoles.

Western Blot Analysis^[2]

Cell Line:

HepG2215 cells
Concentration:

4 μM
Incubation Time:

0, 6, 12, 24 and 36 h
Result:

Decreased the expression of procaspase-3.

In Vivo

Pseudolaric Acid B (Oral gavage; 10-20 mg/kg; 3 days) has anti-fertility ability in rats, which may be related to the maternal vascular system^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 82508-31-4
Appearance Solid
Molecular Weight 432.46
Formula C₂₃H₂₈O₈
Color White to off-white
SMILES

O=C(C1=CC[C@@]([C@@](CC1)(OC(C)=O)[C@]2([H])[C@@](C)(/C=C/C=C(C(O)=O)\C)O3)(CC2)C3=O)OC
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
- Plants
- Pinaceae
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (2)

All

Sort by:

Journal Impact Factor

ACS Infect Dis

Pseudolaric Acid B Ameliorates Fungal Keratitis Progression by Suppressing Inflammation and Reducing Fungal Load. [Abstract]

2023 Jun 9;9(6):1196-1205. PMID: 37141176
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (115.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3124 mL

11.5618 mL

23.1235 mL

5 mM

0.4625 mL

2.3124 mL

4.6247 mL

10 mM

0.2312 mL

1.1562 mL

2.3124 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 10 mg/mL (23.12 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (643 KB)
- English - EN (643 KB)
- Français - FR (643 KB)
- Deutsch - DE (643 KB)
- Norwegian - NO (643 KB)
- Español - ES (643 KB)
- Swedish - SV (643 KB)
- Italian - IT (643 KB)
- Portuguese - PT (643 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yu J, et al. Pseudolaric acid B activates autophagy in MCF-7 human breast cancer cells to prevent cell death. Oncol Lett. 2016 Mar;11(3):1731-1737. [Content Brief]

[2]. Yu J, et al. Pseudolaric acid B inhibits the secretion of hepatitis B virus. Oncol Rep. 2017 Jan;37(1):519-525. [Content Brief]

[3]. Li MH, et al. Pseudolaric acid B inhibits angiogenesis and reduces hypoxia-inducible factor 1alpha by promoting proteasome-mediated degradation. Clin Cancer Res. 2004 Dec 15;10(24):8266-74. [Content Brief]

[4]. Wang WC, et al. [Endocrine activity of pseudolaric acids A and B and their effects on sex hormones, prostaglandins, uteri, and fetuses]. Zhongguo Yao Li Xue Bao. 1991 Mar;12(2):187-90. [Content Brief]

[5]. Wei N, et al. The immunosuppressive activity of pseudolaric acid B on T lymphocytes in vitro. Phytother Res. 2013 Jul;27(7):980-5. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3124 mL	11.5618 mL	23.1235 mL	57.8088 mL
	5 mM	0.4625 mL	2.3124 mL	4.6247 mL	11.5618 mL
	10 mM	0.2312 mL	1.1562 mL	2.3124 mL	5.7809 mL
	15 mM	0.1542 mL	0.7708 mL	1.5416 mL	3.8539 mL
	20 mM	0.1156 mL	0.5781 mL	1.1562 mL	2.8904 mL
	25 mM	0.0925 mL	0.4625 mL	0.9249 mL	2.3124 mL
	30 mM	0.0771 mL	0.3854 mL	0.7708 mL	1.9270 mL
	40 mM	0.0578 mL	0.2890 mL	0.5781 mL	1.4452 mL
	50 mM	0.0462 mL	0.2312 mL	0.4625 mL	1.1562 mL
	60 mM	0.0385 mL	0.1927 mL	0.3854 mL	0.9635 mL
	80 mM	0.0289 mL	0.1445 mL	0.2890 mL	0.7226 mL
	100 mM	0.0231 mL	0.1156 mL	0.2312 mL	0.5781 mL

Keywords

Pseudolaric Acid B

2 Cited Publications

100 mg Get Quote

Subscribe to the

MedChemExpress

Newsletter

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Pseudolaric Acid B

Anti-Infection Compound Library

Apoptosis Compound Library

Epigenetics Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

Natural Product Library

Anti-Cancer Compound Library

Antiviral Compound Library

Autophagy Compound Library

Oxygen Sensing Compound Library

Anti-Cardiovascular Disease Compound Library

Antifungal Compound Library

Ferroptosis Compound Library

Terpenoids Library

Orally Active Compound Library

Glutamine Metabolism Compound Library

Traditional Chinese Medicine Active Compound Library

Anti-Breast Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Angiogenesis-Related Compound Library

Transcription Factor-Targeted Library

Anti-Cancer Natural Product Library

Antiviral Traditional Chinese Medicine Active Compound Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Metabolic Enzyme Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

Lactic Acid Metabolic Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

RNA Binding Bioactive Compound Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Pseudolaric Acid B

All Interleukin Related Isoforms

All HIF/HIF Prolyl-Hydroxylase Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Pseudolaric Acid B Related Classifications

Cat. No.: HY-N6939