Biotinylated Stealth magnetoliposomes

  • Chem Phys Lipids. 2002 Dec;120(1-2):75-85. doi: 10.1016/s0009-3084(02)00105-6.
Michael Hodenius  1 ,  Marcel De Cuyper ,  Linda Desender ,  Detlef Müller-Schulte ,  Alois Steigel ,  Heiko Lueken
Affiliations
  • 1. Rheinisch-Westfälische Technische Hochschule Aachen, Prof. Pirlet-Strasse 1, D-52074, Aachen, Germany.
PMID: 12426077 DOI: 10.1016/s0009-3084(02)00105-6
Abstract

Dimyristoylphosphatidylethanolamine (DC(14:0)PE) and the dioleoyl analogue (DC(18:1cis)PE) were mixed with alpha-biotinylamido-omega-N-succinimidoxycarbonyl-poly(ethylene glycol) (NHS-PEG-biotin) and quantitatively converted to alpha-biotinylamido-omega-(dimyristoylphosphatidylethanolamino-carbonyl)polyethylene glycol (DC(14:0)PE-PEG-biotin) and the dioleoyl analogue DC(18:1cis)PE-PEG-biotin, respectively. As shown by thin-layer chromatography and 1H NMR spectroscopy, PEGylation of both phosphatidylethanolamine types went to completion if the reaction was performed in organic solvent in the presence of triethylamine. The resulting derivatives were successfully incorporated into both classical phospholipid vesicles and a phospholipid bilayer surrounding nanometer-sized magnetite cores. In the latter case, the so-called activated Stealth(1) magnetoliposomes were produced which very efficiently immobilized streptavidinylated Alkaline Phosphatase.

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