Effects of strigolactone-biosynthesis inhibitor TIS108 on Arabidopsis

  • Plant Signal Behav. 2013 May;8(5):e24193. doi: 10.4161/psb.24193.
Shinsaku Ito  1 ,  Mikihisa Umehara ,  Atsushi Hanada ,  Shinjiro Yamaguchi ,  Tadao Asami
Affiliations
  • 1. Department of Applied Biological Chemistry; The University of Tokyo; Tokyo, Japan; Department of Bioscience; Faculty of Applied Bioscience; Tokyo University of Agriculture; Tokyo, Japan.
PMID: 23511201 DOI: 10.4161/psb.24193
Abstract

TIS108 is a triazole-type strigolactone (SL)-biosynthesis inhibitor that reduces the level of 2'-epi-5-deoxystrigol (epi-5DS) in rice. Here we report the effects of TIS108 on Arabidopsis. Treatment of TIS108 increased the number of branches and repressed root hair elongation as was observed in SL-deficient mutants, and co-application of GR24, a synthetic SL analog, recovered the TIS108-induced phenotype to that of wild-type. In addition, MAX3 and MAX4 genes in the SL-biosynthesis pathway were upregulated in TIS108-treated Arabidopsis, probably due to feedback regulation caused by SL deficiency. These results indicate that TIS108 is an effective tool for regulating SL production in Arabidopsis.

Keywords
arabidopsis; biosynthesis inhibitor; plant growth regulator; plant hormone; strigolactone.
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