Analysis of Chemical Variations between Crude and Salt-Processed Anemarrhenae rhizoma Using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry Methods

  • Molecules. 2017 Dec 22;23(1):23. doi: 10.3390/molecules23010023.
De Ji  1  2  3 ,  Xiaonan Su  4  5  6 ,  Ziyan Huang  7  8  9 ,  Lialin Su  10  11  12 ,  Lin Li  13  14  15 ,  Tulin Lu  16  17  18
Affiliations
  • 1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 2. State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 3. Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 4. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 5. State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 6. Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 7. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 8. State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 9. Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 10. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 11. State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 12. Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 13. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 14. State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 15. Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 16. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 17. State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
  • 18. Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
PMID: 29271935 DOI: 10.3390/molecules23010023
Abstract

The present study was designed to systematically investigate the chemical profile differences between crude Anemarrhenae rhizoma (CAR) and salt-processed Anemarrhenae rhizoma (SAR). Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS), coupled with multivariate statistical analysis was used for the discrimination of chemical profiles and the identification of the differentiation of the chemical constitutions of CAR and SAR. In addition, seven main constituents of CAR and SAR were simultaneously determined by ultra-high-performance liquid chromatography-quadrupole mass spectrometry (UHPLC-MS) for analyzing the content variations. A total of 24 components were found to be the main contributors to the significant difference between CAR and SAR. The structures of the marker compounds were identified based on their chromatographic behaviors, intact precursor ions, and characteristic MS fragmentation patterns. The potential structural transformation mechanism of furostanol saponins during salt processing was explored. The results may provide a scientific foundation for deeply elucidating the processing mechanism of Anemarrhenae rhizoma.

Keywords
Anemarrhenae rhizoma; UPLC–QTOF-MS; chemical profile; furostanol saponins; salt processing.
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