Didemnin B induces cell death by apoptosis: the fastest induction of apoptosis ever described

  • Biochem Biophys Res Commun. 1995 Oct 24;215(3):1130-6. doi: 10.1006/bbrc.1995.2580.
D R Grubb  1 ,  E J Wolvetang ,  A Lawen
Affiliations
  • 1. Department of Biochemistry and Molecular Biology, Monash University, Clayton (Melbourne) Vic, Australia.
PMID: 7488040 DOI: 10.1006/bbrc.1995.2580
Abstract

Didemnin B, a cyclic N-methylated peptolide induces Apoptosis in human HL-60 cells. When incubated with 1 microM didemnin B, unsynchronized HL-60 cultures undergo Apoptosis to 100% within 140 minutes. Apoptosis has been assessed by the typical apoptotic morphology, the presence of double-stranded DNA fragments within the cytosol and the generation of DNA ladders. None of these characteristics of Apoptosis are seen when HL-60 cells are pretreated with 1mM Zn2+ immediately before treatment with didemnin B.

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