1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Ligases (EC 6)
  4. AARS1 Protein, Mouse (sf9, His, solution)

The AARS1 protein facilitates the two-step process of attaching alanine to tRNA(Ala): first, alanine is activated by ATP to form Ala-AMP, and then it is transferred to the acceptor end of tRNA(Ala). AARS1 also corrects incorrectly charged tRNA(Ala) through its editing domain. AARS1 Protein, Mouse (sf9, His, solution) is the recombinant mouse-derived AARS1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

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  • Biologische Aktivität

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  • Beschreibung

Beschreibung

The AARS1 protein facilitates the two-step process of attaching alanine to tRNA(Ala): first, alanine is activated by ATP to form Ala-AMP, and then it is transferred to the acceptor end of tRNA(Ala). AARS1 also corrects incorrectly charged tRNA(Ala) through its editing domain. AARS1 Protein, Mouse (sf9, His, solution) is the recombinant mouse-derived AARS1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

AARS1, a pivotal enzyme in protein synthesis, plays a crucial role in the attachment of alanine to tRNA(Ala) through a two-step process. Initially, alanine undergoes activation by ATP to form Ala-AMP, and subsequently, it is transferred to the acceptor end of tRNA(Ala). Beyond its primary aminoacylation function, AARS1 boasts an editing domain that enables the correction of incorrectly charged tRNA(Ala), underscoring its role in maintaining the fidelity of the translation process. This dual functionality highlights AARS1's significance in ensuring the accuracy and integrity of the protein synthesis machinery.

Biologische Aktivität

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Mouse

Source

Sf9 insect cells

Tag

C-8*His

Accession

Q8BGQ7 (M1-N968)

Gene ID

234734

Molecular Construction
N-term
AARS1 (M1-N968)
Accession # Q8BGQ7
8*His
C-term
Protein Length

Full Length

Synonyms
Alanine--tRNA ligase, cytoplasmic; Alanyl-tRNA synthetase; AlaRS; Sti; Aars
AA Sequence

MDATLTAREIRERFINFFRRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPVERLYVTYFGGDEAAGLEPDLECRQIWQNLGLDEARILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNRESDGVLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFMPYFEAIQKGTGARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYSHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPEMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDCKTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMNGFEEERRLAQLKSQGKGAGDEDLIMLDIYAIEELRAKGLEATDDSPKYNYQSDSSGSYVFECTVATVLALRREKMFVDEVVTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGNLKVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVLGEADQKGSLVAPDRLRFDFTAKGAMSTQQIKKAEEIVNGMIEAAKPVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPCGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKSETLKKSLSAMEAKVKAQTAPNKDVQREIADLGEALATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKRVLEKTKQLIDSNPNQPLVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQVSGLMDGKGGGKDMSAQATGKNVGCLQEALQLATSFAQLRLGDVKN

Molekulargewicht

Approximately 108.43 kDa

Reinheit

≥ 95%, as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Versand

Shipping with dry ice.

Dokumentation

AARS1 Protein, Mouse (sf9, His, solution) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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AARS1 Protein, Mouse (sf9, His, solution)

 

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Produktname:
AARS1 Protein, Mouse (sf9, His, solution)
Art. -Nr.:
HY-P75567Y
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