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ABAT Protein, Human (His, SUMO, Myc)

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ABAT Protein, Human (His, SUMO, Myc) is a γ-Aminobutyric acid transaminase which is responsible for GABA metabolism. ABAT, Human is implicated in mitochondrial nucleoside metabolism, respiratory capacity, membrane potential, and mitochondrial DNA maintenance.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • ABAT Protein, Human (His, SUMO, Myc) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Biological Activity

Description

ABAT Protein, Human (His, SUMO, Myc) is a γ-Aminobutyric acid transaminase which is responsible for GABA metabolism. ABAT, Human is implicated in mitochondrial nucleoside metabolism, respiratory capacity, membrane potential, and mitochondrial DNA maintenance.

Background

GABA transaminase (ABAT) is the protein responsible for GABA metabolism. ABAT mediates the GABA metabolic pathway, a bypass mechanism in the tricarboxylic acid cycle (TCAC), producing succinate and NADH. γ-Aminobutyric acid (GABA), routinely described as an inhibitory neurotransmitter, is found in high concentrations in the neural parenchyma and has various roles in the CNS. Elevated ABAT expression results in increased GABA catabolism, decreased tumor cell proliferation. In mammals, reduced ABAT expression is implicated in primary clear cell renal carcinoma, breast cancer, and myelodysplastic syndrome tumorigenesis. ABAT has been shown to be implicated in mitochondrial nucleoside metabolism, respiratory capacity, membrane potential, and mitochondrial DNA maintenance[1].

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag C-Myc;N-10*His;N-SUMO
  • Accession

    P80404 (S29-K500)

  • Gene ID
    18  [NCBI]
  • Molecular Construction
    • N-term
    • 10*His-SUMO
    • ABAT (S29-K500)
      Accession # P80404
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    ABAT; GABA-T; 4-Aminobutyrate Aminotransferase; Gamma-Aminobutyrate Aminotransferase; GABAT; 4-Aminobutyrate Transaminase; 4-Aminobutyrate Aminotransferase, Mitochondrial; GABA-AT; (S)-3-Amino-2-Methylpropionate Transaminase; L-AIBAT; Gamma-Amino-N-Butyra

  • AA Sequence

    SQAAAKVDVEFDYDGPLMKTEVPGPRSQELMKQLNIIQNAEAVHFFCNYEESRGNYLVDVDGNRMLDLYSQISSVPIGYSHPALLKLIQQPQNASMFVNRPALGILPPENFVEKLRQSLLSVAPKGMSQLITMACGSCSNENALKTIFMWYRSKERGQRGFSQEELETCMINQAPGCPDYSILSFMGAFHGRTMGCLATTHSKAIHKIDIPSFDWPIAPFPRLKYPLEEFVKENQQEEARCLEEVEDLIVKYRKKKKTVAGIIVEPIQSEGGDNHASDDFFRKLRDIARKHGCAFLVDEVQTGGGCTGKFWAHEHWGLDDPADVMTFSKKMMTGGFFHKEEFRPNAPYRIFNTWLGDPSKNLLLAEVINIIKREDLLNNAAHAGKALLTGLLDLQARYPQFISRVRGRGTFCSFDTPDDSIRNKLILIARNKGVVLGGCGDKSIRFRPTLVFRDHHAHLFLNIFSDILADFK

  • Molecular Weight

    Approximately 69-76 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Martirosian V, et, al. Medulloblastoma uses GABA transaminase to survive in the cerebrospinal fluid microenvironment and promote leptomeningeal dissemination. Cell Rep. 2021 Jun 29;35(13):109302. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • ABAT Protein, Human (His, SUMO, Myc) Product Image