ACPL2 Protein, Human (HEK293, His)
The ACPL2 protein plays a critical role in the regulation of glycosaminoglycan (GAG) chains by catalyzing the 2-O-dephosphorylation of xylose in proteoglycans. This step is critical for the synthesis of sulfated GAGs, including heparan sulfate and chondroitin sulfate. ACPL2 Protein, Human (HEK293, His) is the recombinant human-derived ACPL2 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The ACPL2 protein plays a critical role in the regulation of glycosaminoglycan (GAG) chains by catalyzing the 2-O-dephosphorylation of xylose in proteoglycans. This step is critical for the synthesis of sulfated GAGs, including heparan sulfate and chondroitin sulfate. ACPL2 Protein, Human (HEK293, His) is the recombinant human-derived ACPL2 protein, expressed by HEK293 , with C-His labeled tag.
Background
The ACPL2 protein assumes a crucial role in the regulation of mature glycosaminoglycan (GAG) chains by catalyzing the 2-O-dephosphorylation of xylose within the glycosaminoglycan-protein linkage region of proteoglycans. This process holds significance in the synthesis of sulfated GAGs, such as heparan sulfate and chondroitin sulfate, on the common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins. The stepwise addition of monosaccharide residues by specific glycosyltransferases leads to the formation of this linkage region, with xylose 2-O-dephosphorylation serving as a prerequisite for the initiation and efficient elongation of the repeating disaccharide region of GAG chains. ACPL2's enzymatic activity in this context underscores its pivotal role in the fine-tuned control of GAG chain assembly, contributing to the precise regulation of glycosaminoglycan structures.
Technical Parameters
-
Species Human
-
Source HEK293
-
Tag C-His
-
Accession
Q8TE99-1/NP_689495.1 (M1-F480)
-
Gene ID92370 [NCBI]
-
Molecular Construction
-
N-term
-
ACPL2 (M1-F480)
Accession # Q8TE99-1/NP_689495.1 -
His
-
C-term
-
-
Protein Length
Full Length of Isoform-1
-
Synonyms
PXYLP1; Acid Phosphatase-Like 2; Prev. ACPL2; FLJ23751; Acid Phosphatase-Like Protein 2; HEL124; Epididymis Luminal Protein 124; XYLP; 2-Phosphoxylose Phosphatase 1; Xylosyl Phosphatase
-
AA Sequence
MLFRNRFLLLLALAALLAFVSLSLQFFHLIPVSTPKNGMSSKSRKRIMPDPVTEPPVTDPVYEALLYCNIPSVAERSMEGHAPHHFKLVSVHVFIRHGDRYPLYVIPKTKRPEIDCTLVANRKPYHPKLEAFISHMSKGSGASFESPLNSLPLYPNHPLCEMGELTQTGVVQHLQNGQLLRDIYLKKHKLLPNDWSADQLYLETTGKSRTLQSGLALLYGFLPDFDWKKIYFRHQPSALFCSGSCYCPVRNQYLEKEQRRQYLLRLKNSQLEKTYGEMAKIVDVPTKQLRAANPIDSMLCHFCHNVSFPCTRNGCVDMEHFKVIKTHQIEDERERREKKLYFGYSLLGAHPILNQTIGRMQRATEGRKEELFALYSAHDVTLSPVLSALGLSEARFPRFAARLIFELWQDREKPSEHSVRILYNGVDVTFHTSFCQDHHKRSPKPMCPLENLVRFVKRDMFVALGGSGTNYYDACHREGF
-
Predicted Molecular Mass
54 kDa
-
Molecular Weight
Approximately 50 kDa, based on SDS-PAGE under reducing conditions.
-
Glycosylation
Yes
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
