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Aldose-reductase Protein, Human

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The Aldose-reductase protein reduces carbonyl-containing compounds to alcohols, including retinals. It detoxifies unsaturated carbonyls like crotonaldehyde and 4-hydroxynonenal, but lacks reductase activity towards glucose. Aldose-reductase Protein, Human is the recombinant human-derived Aldose-reductase protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The Aldose-reductase protein reduces carbonyl-containing compounds to alcohols, including retinals. It detoxifies unsaturated carbonyls like crotonaldehyde and 4-hydroxynonenal, but lacks reductase activity towards glucose. Aldose-reductase Protein, Human is the recombinant human-derived Aldose-reductase protein, expressed by E. coli , with tag free.

Background

Aldose reductase, a multifunctional enzyme, catalyzes the NADPH-dependent reduction of a diverse range of carbonyl-containing compounds, converting them into their corresponding alcohols. Notably, it exhibits robust enzymatic activity towards various retinal isomers, including all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. The enzyme plays a pivotal role in the detoxification of unsaturated carbonyls derived from the diet and lipids, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal, and their glutathione-conjugates carbonyls (GS-carbonyls). Remarkably, despite its broad substrate specificity, Aldose reductase does not display reductase activity towards glucose. This diverse catalytic repertoire highlights its significance in cellular detoxification processes and underscores its potential therapeutic implications in addressing oxidative stress-related conditions.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    O60218 (M1-Y316)

  • Gene ID
    57016
  • Molecular Construction
    • N-term
    • Aldose-reductase (M1-Y316)
      Accession # O60218
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    AKR1B10; HSI; Prev. AKR1B11; Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase); ARL-1; Aldose Reductase-Like Peptide; Aldose Reductase-Related Protein; Aldose Reductase-Like 1; Small Intestine Reductase; SI Reductase; AKR1B12; HARP; ALDRLn; ARP;

  • AA Sequence

    MATFVELSTKAKMPIVGLGTWKSPLGKVKEAVKVAIDAGYRHIDCAYVYQNEHEVGEAIQEKIQEKAVKREDLFIVSKLWPTFFERPLVRKAFEKTLKDLKLSYLDVYLIHWPQGFKSGDDLFPKDDKGNAIGGKATFLDAWEAMEELVDEGLVKALGVSNFSHFQIEKLLNKPGLKYKPVTNQVECHPYLTQEKLIQYCHSKGITVTAYSPLGSPDRPWAKPEDPSLLEDPKIKEIAAKHKKTAAQVLIRFHIQRNVIVIPKSVTPARIVENIQVFDFKLSDEEMATILSFNRNWRACNVLQSSHLEDYPFNAEY

  • Molecular Weight

    Approximately 36 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
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ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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