Aldose-reductase Protein, Human

The Aldose-reductase protein reduces carbonyl-containing compounds to alcohols, including retinals. It detoxifies unsaturated carbonyls like crotonaldehyde and 4-hydroxynonenal, but lacks reductase activity towards glucose. Aldose-reductase Protein, Human is the recombinant human-derived Aldose-reductase protein, expressed by E. coli , with tag free.

Aldose reductase, a multifunctional enzyme, catalyzes the NADPH-dependent reduction of a diverse range of carbonyl-containing compounds, converting them into their corresponding alcohols. Notably, it exhibits robust enzymatic activity towards various retinal isomers, including all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. The enzyme plays a pivotal role in the detoxification of unsaturated carbonyls derived from the diet and lipids, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal, and their glutathione-conjugates carbonyls (GS-carbonyls). Remarkably, despite its broad substrate specificity, Aldose reductase does not display reductase activity towards glucose. This diverse catalytic repertoire highlights its significance in cellular detoxification processes and underscores its potential therapeutic implications in addressing oxidative stress-related conditions.

Human

E. coli

Tag Free

O60218 (M1-Y316)

57016

Full Length

MATFVELSTKAKMPIVGLGTWKSPLGKVKEAVKVAIDAGYRHIDCAYVYQNEHEVGEAIQEKIQEKAVKREDLFIVSKLWPTFFERPLVRKAFEKTLKDLKLSYLDVYLIHWPQGFKSGDDLFPKDDKGNAIGGKATFLDAWEAMEELVDEGLVKALGVSNFSHFQIEKLLNKPGLKYKPVTNQVECHPYLTQEKLIQYCHSKGITVTAYSPLGSPDRPWAKPEDPSLLEDPKIKEIAAKHKKTAAQVLIRFHIQRNVIVIPKSVTPARIVENIQVFDFKLSDEEMATILSFNRNWRACNVLQSSHLEDYPFNAEY

Approximately 36 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.