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Annexin A3/ANXA3 Protein, Human

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Annexin A3 Protein, Human is a 37 kDa endogenous and secretory multifunctional protein which belongs to the annexin family. Annexin A13 is highly expressed in various carcinomas cells. Human Annexin A3 can promote aggressive cancer and stem cell-like properties and can be used as a useful predictive biomarker in HCC.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Annexin A3/ANXA3 Protein, Human
  • Annexin A3/ANXA3 Protein, Human Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Biological Activity

Description

Annexin A3 Protein, Human is a 37 kDa endogenous and secretory multifunctional protein which belongs to the annexin family. Annexin A13 is highly expressed in various carcinomas cells. Human Annexin A3 can promote aggressive cancer and stem cell-like properties and can be used as a useful predictive biomarker in HCC[1].

Background

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins.
Annexin A3, also known as Annexin III and Lipocortin III (LPC3), is a calcium-dependent phospholipid-binding protein being preferentially located on the cytosolic face of the plasma membrane. Human Annexin A3 shares 89% and 85% aa sequence identity with mouse and rat Annexin A3, respectively[1][2].

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P12429 (M1-D323)

  • Gene ID
    306  [NCBI]
  • Molecular Construction
    • N-term
    • ANXA3 (M1-D323)
      Accession # P12429
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    ANXA3; Annexin-3; Prev. ANX3; Annexin III (Lipocortin III, 1,2-Cyclic-Inositol-Phosphate Phosphodiesterase, Placental Anticoagulant Protein III, Calcimedin 35-Alpha); Inositol 1,2-Cyclic Phosphate 2-Phosphohydrolase; Annexin III (Lipocortin III); Placenta

  • AA Sequence

    MASIWVGHRGTVRDYPDFSPSVDAEAIQKAIRGIGTDEKMLISILTERSNAQRQLIVKEYQAAYGKELKDDLKGDLSGHFEHLMVALVTPPAVFDAKQLKKSMKGAGTNEDALIEILTTRTSRQMKDISQAYYTVYKKSLGDDISSETSGDFRKALLTLADGRRDESLKVDEHLAKQDAQILYKAGENRWGTDEDKFTEILCLRSFPQLKLTFDEYRNISQKDIVDSIKGELSGHFEDLLLAIVNCVRNTPAFLAERLHRALKGIGTDEFTLNRIMVSRSEIDLLDIRTEFKKHYGYSLYSAIKSDTSGDYEITLLKICGGDD

  • Predicted Molecular Mass

    36.4 kDa

  • Molecular Weight

    Approximately 33 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Man Tong, et al. Efficacy of annexin A3 blockade in sensitizing hepatocellular carcinoma to sorafenib and regorafenib. J Hepatol. 2018 Oct;69(4):826-839. [Content Brief]

[2]. Juan Wang, et al. Annexin A3 may play an important role in ochratoxin-induced malignant transformation of human gastric epithelium cells. Toxicol Lett. 2019 Oct 1;313:150-158. [Content Brief]

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
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ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • Annexin A3/ANXA3 Protein, Human Product Image
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  • Experimental Validation Results for Annexin A3/ANXA3 Protein, Human
  • Annexin A3/ANXA3 Protein, Human Product Image