Annexin A7/ANXA7 Protein, Human
Based on 1 Customer Validation
Annexin A7 Protein, Human is a 51 kDa multifunctional protein which belongs to the the group A annexin family and contains an extraordinarily long amino terminus. Annexin A7 is a membrane binding protein with diverse properties including voltage sensitive calcium channel activity, ion selectivity and membrane fusion.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
Annexin A7 Protein, Human is a 51 kDa multifunctional protein which belongs to the the group A annexin family and contains an extraordinarily long amino terminus. Annexin A7 is a membrane binding protein with diverse properties including voltage sensitive calcium channel activity, ion selectivity and membrane fusion[1][2].
Background
The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins.Annexin A7 is encoded by ANXA7 gene and is the first isolated annexin, mediating the Ca2+-regulated chromaffin granule exocytosis. Annexin 7 has molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. Annexin A7 plays an important role in membrane fusion during exocytosis, localizing predominantly in the cytoplasm and showing a high prevalence in the brain, heart, and skeletal muscle[1].
Verified Bioactivity
Immobilized Human Annexin A7 Protein at 5 μg/mL (100 μL/well) can bind Anti-Annexin A7 antibody. The ED50 for this effect is 3.01 ng/mL.
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
P20073-2 (M1-Q466)
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Molecular Construction
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N-term
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ANXA7 (M1-Q466)
Accession # P20073-2 -
C-term
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Protein Length
Full Length of Isoform-2
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Synonyms
ANXA7; Annexin-7; Prev. ANX7; SYNEXIN; Annexin VII; Synexin; Annexin A7; SNX
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AA Sequence
MSYPGYPPTGYPPFPGYPPAGQESSFPPSGQYPYPSGFPPMGGGAYPQVPSSGYPGAGGYPAPGGYPAPGGYPGAPQPGGAPSYPGVPPGQGFGVPPGGAGFSGYPQPPSQSYGGGPAQVPLPGGFPGGQMPSQYPGGQPTYPSQPATVTQVTQGTIRPAANFDAIRDAEILRKAMKGFGTDEQAIVDVVANRSNDQRQKIKAAFKTSYGKDLIKDLKSELSGNMEELILALFMPPTYYDAWSLRKAMQGAGTQERVLIEILCTRTNQEIREIVRCYQSEFGRDLEKDIRSDTSGHFERLLVSMCQGNRDENQSINHQMAQEDAQRLYQAGEGRLGTDESCFNMILATRSFPQLRATMEAYSRMANRDLLSSVSREFSGYVESGLKTILQCALNRPAFFAERLYYAMKGAGTDDSTLVRIVVTRSEIDLVQIKQMFAQMYQKTLGTMIAGDTSGDYRRLLLAIVGQ
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Predicted Molecular Mass
50.3 kDa
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Molecular Weight
Approximately 47 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 100 mM NaCl, 8% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (264 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Weihua Ye, et al. Effect of annexin A7 suppression on the apoptosis of gastric cancer cells. Mol Cell Biochem. 2017 May;429(1-2):33-43. [Content Brief]
[2]. Hu-Fang Yuan, et al. Downregulation of annexin A7 decreases proliferation, migration, and invasion of gastric cancer cells by reducing matrix metalloproteinase 1 and 9 expression. Am J Transl Res. 2019 May 15;11(5):2754-2764. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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