AQP2 Protein, Mouse (Cell-Free, His)
AQP2 is a homotetrameric protein that plays a key role in renal water homeostasis. It acts as a water-specific channel, facilitating the movement of water across the plasma membrane of the renal collecting duct. AQP2 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived AQP2 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
- Species: Mouse
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Biological Activity
Description
AQP2 is a homotetrameric protein that plays a key role in renal water homeostasis. It acts as a water-specific channel, facilitating the movement of water across the plasma membrane of the renal collecting duct. AQP2 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived AQP2 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
Background
AQP2 is a homotetrameric protein that plays a key role in renal water homeostasis. It acts as a water-specific channel, facilitating the movement of water across the plasma membranes of renal collecting ducts. By conferring high permeability to water, AQP2 enables water to flow in response to osmotic gradients, thus contributing to the regulation of water balance in the kidneys. Its crucial function in maintaining proper water levels underscores its significance in renal physiology and highlights its importance in overall body fluid regulation.
Technical Parameters
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Species Mouse
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Source E. coli Cell-free
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Tag N-10*His
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Accession
P56402 (M1-A271)
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Gene ID11827
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Molecular Construction
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N-term
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10*His
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AQP2 (M1-A271)
Accession # P56402 -
C-term
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Protein Length
Full Length
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Synonyms
AQP2; WCH-CD; Aquaporin 2; Aquaporin 2 (Collecting Duct); Water Channel Protein For Renal Collecting Duct; AQP-CD; Collecting Duct Water Channel Protein; AQP-2; ADH Water Channel; Water-Channel Aquaporin 2; Aquaporin-CD; Aquaporin-2 Mutant; Aquaporin-2; N
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AA Sequence
MWELRSIAFSRAVLAEFLATLLFVFFGLGSALQWASSPPSVLQIAVAFGLGIGTLVQALGHVSGAHINPAVTVACLVGCHVSFLRAAFYVAAQLLGAVAGAAILHEITPVEIRGDLAVNALHNNATAGQAVTVELFLTMQLVLCIFASTDERRSDNLGSPALSIGFSVTLGHLLGIYFTGCSMNPARSLAPAVVTGKFDDHWVFWIGPLVGAVIGSLLYNYLLFPSTKSLQERLAVLKGLEPDTDWEEREVRRRQSVELHSPQSLPRGSKA
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Predicted Molecular Mass
30.5 kDa
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)