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AQP2 Protein, Mouse (Cell-Free, His)

Cat. No.: HY-P702213
Handling Instructions

AQP2 is a homotetrameric protein that plays a key role in renal water homeostasis. It acts as a water-specific channel, facilitating the movement of water across the plasma membrane of the renal collecting duct. AQP2 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived AQP2 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of AQP2 Protein, Mouse (Cell-Free, His) is 271 a.a., with molecular weight of 30.5 kDa.

For research use only. We do not sell to patients.

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Description

AQP2 is a homotetrameric protein that plays a key role in renal water homeostasis. It acts as a water-specific channel, facilitating the movement of water across the plasma membrane of the renal collecting duct. AQP2 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived AQP2 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of AQP2 Protein, Mouse (Cell-Free, His) is 271 a.a., with molecular weight of 30.5 kDa.

Background

AQP2 is a homotetrameric protein that plays a key role in renal water homeostasis. It acts as a water-specific channel, facilitating the movement of water across the plasma membranes of renal collecting ducts. By conferring high permeability to water, AQP2 enables water to flow in response to osmotic gradients, thus contributing to the regulation of water balance in the kidneys. Its crucial function in maintaining proper water levels underscores its significance in renal physiology and highlights its importance in overall body fluid regulation.

Species

Mouse

Source

E. coli Cell-free

Tag

N-10*His

Accession

P56402 (M1-A271)

Gene ID

11827

Molecular Construction
N-term
10*His
AQP2 (M1-A271)
Accession # P56402
C-term
Synonyms
Aquaporin-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct
AA Sequence

MWELRSIAFSRAVLAEFLATLLFVFFGLGSALQWASSPPSVLQIAVAFGLGIGTLVQALGHVSGAHINPAVTVACLVGCHVSFLRAAFYVAAQLLGAVAGAAILHEITPVEIRGDLAVNALHNNATAGQAVTVELFLTMQLVLCIFASTDERRSDNLGSPALSIGFSVTLGHLLGIYFTGCSMNPARSLAPAVVTGKFDDHWVFWIGPLVGAVIGSLLYNYLLFPSTKSLQERLAVLKGLEPDTDWEEREVRRRQSVELHSPQSLPRGSKA

Molecular Weight

30.5 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

AQP2 Protein, Mouse (Cell-Free, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
AQP2 Protein, Mouse (Cell-Free, His)
Cat. No.:
HY-P702213
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