AQP2 Protein, Mouse (Cell-Free, His)

AQP2 is a homotetrameric protein that plays a key role in renal water homeostasis. It acts as a water-specific channel, facilitating the movement of water across the plasma membrane of the renal collecting duct. AQP2 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived AQP2 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli Cell-free
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

AQP2 is a homotetrameric protein that plays a key role in renal water homeostasis. It acts as a water-specific channel, facilitating the movement of water across the plasma membrane of the renal collecting duct. AQP2 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived AQP2 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.

Background

AQP2 is a homotetrameric protein that plays a key role in renal water homeostasis. It acts as a water-specific channel, facilitating the movement of water across the plasma membranes of renal collecting ducts. By conferring high permeability to water, AQP2 enables water to flow in response to osmotic gradients, thus contributing to the regulation of water balance in the kidneys. Its crucial function in maintaining proper water levels underscores its significance in renal physiology and highlights its importance in overall body fluid regulation.

Technical Parameters

  • Species Mouse
  • Source E. coli Cell-free
  • Tag N-10*His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • 10*His
    • AQP2 (M1-A271)
      Accession # P56402
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    AQP2; WCH-CD; Aquaporin 2; Aquaporin 2 (Collecting Duct); Water Channel Protein For Renal Collecting Duct; AQP-CD; Collecting Duct Water Channel Protein; AQP-2; ADH Water Channel; Water-Channel Aquaporin 2; Aquaporin-CD; Aquaporin-2 Mutant; Aquaporin-2; N

  • AA Sequence

    MWELRSIAFSRAVLAEFLATLLFVFFGLGSALQWASSPPSVLQIAVAFGLGIGTLVQALGHVSGAHINPAVTVACLVGCHVSFLRAAFYVAAQLLGAVAGAAILHEITPVEIRGDLAVNALHNNATAGQAVTVELFLTMQLVLCIFASTDERRSDNLGSPALSIGFSVTLGHLLGIYFTGCSMNPARSLAPAVVTGKFDDHWVFWIGPLVGAVIGSLLYNYLLFPSTKSLQERLAVLKGLEPDTDWEEREVRRRQSVELHSPQSLPRGSKA

  • Predicted Molecular Mass

    30.5 kDa

  • Purity

    ≥ 85%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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