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AQP4 Protein, Mouse (Cell-Free, His)

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The AQP4 protein forms water-specific channels that are critical for brain water homeostasis and lymphoid solute transport. It regulates water exchange at the blood-brain interface, promoting the influx of cerebrospinal fluid into the brain and the drainage of interstitial fluid. AQP4 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived AQP4 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli Cell-free
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

The AQP4 protein forms water-specific channels that are critical for brain water homeostasis and lymphoid solute transport. It regulates water exchange at the blood-brain interface, promoting the influx of cerebrospinal fluid into the brain and the drainage of interstitial fluid. AQP4 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived AQP4 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.

Background

AQP4 Protein forms a water-specific channel, crucial for brain water homeostasis and glymphatic solute transport. It plays a vital role in the normal rate of water exchange across the blood-brain interface and is necessary for cerebrospinal fluid influx into the brain cortex and parenchyma, along with drainage of interstitial fluid. AQP4 is essential for the clearance of solutes, including soluble beta-amyloid peptides, from the brain interstitial fluid. Additionally, it plays a redundant role in urinary water homeostasis and concentrating ability. Existing as a homotetramer, AQP4 tetramers can create oligomeric arrays in membranes, with varying sizes between tissues. Interactions among AQP4 oligomeric arrays in adjacent cells contribute to cell-cell adhesion, and it is part of a complex containing MLC1, TRPV4, HEPACAM, and ATP1B1.

Technical Parameters

  • Species Mouse
  • Source E. coli Cell-free
  • Tag N-10*His
  • Accession

    P55088 (M1-V323)

  • Gene ID
    11829
  • Molecular Construction
    • N-term
    • 10*His
    • AQP4 (M1-V323)
      Accession # P55088
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    AQP4; HAQP4; Aquaporin 4; WCH4; MIWC; Aquaporin Type4 Transcript Variant C; Mercurial-Insensitive Water Channel; Alternative Protein AQP4; Aquaporin-4; Aquaporin Type4; AQP-4; MLC4

  • AA Sequence

    MSDGAAARRWGKCGHSCSRESIMVAFKGVWTQAFWKAVSAEFLATLIFVLLGVGSTINWGGSENPLPVDMVLISLCFGLSIATMVQCFGHISGGHINPAVTVAMVCTRKISIAKSVFYIIAQCLGAIIGAGILYLVTPPSVVGGLGVTTVHGNLTAGHGLLVELIITFQLVFTIFASCDSKRTDVTGSIALAIGFSVAIGHLFAINYTGASMNPARSFGPAVIMGNWANHWIYWVGPIMGAVLAGALYEYVFCPDVELKRRLKEAFSKAAQQTKGSYMEVEDNRSQVETEDLILKPGVVHVIDIDRGEEKKGKDSSGEVLSSV

  • Predicted Molecular Mass

    40.5 kDa

  • Molecular Weight

    Approximately 38 kDa (Monomer) & 76 kDa (Dimer), based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.15 M NaCl, 0.05% Brij-78, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Mass (in vial) Mass (in vial)
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Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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