AQP4 Protein, Mouse (Cell-Free, His)

The AQP4 protein forms water-specific channels that are critical for brain water homeostasis and lymphoid solute transport. It regulates water exchange at the blood-brain interface, promoting the influx of cerebrospinal fluid into the brain and the drainage of interstitial fluid. AQP4 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived AQP4 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of AQP4 Protein, Mouse (Cell-Free, His) is 323 a.a., with molecular weight of 40.5 kDa.

AQP4 Protein forms a water-specific channel, crucial for brain water homeostasis and glymphatic solute transport. It plays a vital role in the normal rate of water exchange across the blood-brain interface and is necessary for cerebrospinal fluid influx into the brain cortex and parenchyma, along with drainage of interstitial fluid. AQP4 is essential for the clearance of solutes, including soluble beta-amyloid peptides, from the brain interstitial fluid. Additionally, it plays a redundant role in urinary water homeostasis and concentrating ability. Existing as a homotetramer, AQP4 tetramers can create oligomeric arrays in membranes, with varying sizes between tissues. Interactions among AQP4 oligomeric arrays in adjacent cells contribute to cell-cell adhesion, and it is part of a complex containing MLC1, TRPV4, HEPACAM, and ATP1B1.

Mouse

E. coli Cell-free

N-10*His

P55088 (M1-V323)

11829

MSDGAAARRWGKCGHSCSRESIMVAFKGVWTQAFWKAVSAEFLATLIFVLLGVGSTINWGGSENPLPVDMVLISLCFGLSIATMVQCFGHISGGHINPAVTVAMVCTRKISIAKSVFYIIAQCLGAIIGAGILYLVTPPSVVGGLGVTTVHGNLTAGHGLLVELIITFQLVFTIFASCDSKRTDVTGSIALAIGFSVAIGHLFAINYTGASMNPARSFGPAVIMGNWANHWIYWVGPIMGAVLAGALYEYVFCPDVELKRRLKEAFSKAAQQTKGSYMEVEDNRSQVETEDLILKPGVVHVIDIDRGEEKKGKDSSGEVLSSV

Observed band size: Monomer: 38 kDa Dimer: 76 kDa It is speculated that the protein forms a dimeric structure.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.15 M NaCl, 0.05% Brij-78, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.